Rapid construction of metabolite biosensors using domain-insertion profiling

In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-se...

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Auteurs principaux: Dana C. Nadler, Stacy-Anne Morgan, Avi Flamholz, Kaitlyn E. Kortright, David F. Savage
Format: article
Langue:EN
Publié: Nature Portfolio 2016
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Accès en ligne:https://doaj.org/article/8d05fc327b244948b82af826e79987c9
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