Rapid construction of metabolite biosensors using domain-insertion profiling
In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-se...
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Auteurs principaux: | Dana C. Nadler, Stacy-Anne Morgan, Avi Flamholz, Kaitlyn E. Kortright, David F. Savage |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2016
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Accès en ligne: | https://doaj.org/article/8d05fc327b244948b82af826e79987c9 |
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