CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)

Monolayer tissue cultures of chicken embryo fibroblast ( CEF ) cells infected with avian influenza virus isolate were examined by the hematoxylie and eosin (H&E) staining and indirect immunoperoxidase test for studying the cytopathogenic effect of the virus. Cytopathological changes which occur...

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Autor principal:	H. M. I. AL-HYALI
Formato:	article
Lenguaje:	EN
Publicado:	University of Baghdad, College of Veterinary Medicine 2021
Materias:	INFLUENZA VIRUS (H4 N3) chicken CYTOPATHOGENICITY Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/8d097a5c44d34f5a946d787ec2ac997f
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spelling	oai:doaj.org-article:8d097a5c44d34f5a946d787ec2ac997f2021-11-06T11:33:38ZCYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)10.30539/ijvm.v25i1.11591609-56932410-7409https://doaj.org/article/8d097a5c44d34f5a946d787ec2ac997f2021-11-01T00:00:00Zhttps://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1159https://doaj.org/toc/1609-5693https://doaj.org/toc/2410-7409 Monolayer tissue cultures of chicken embryo fibroblast ( CEF ) cells infected with avian influenza virus isolate were examined by the hematoxylie and eosin (H&E) staining and indirect immunoperoxidase test for studying the cytopathogenic effect of the virus. Cytopathological changes which occurred in the uncleus of infected cells included nuclear and nucleolar hypertropy, chromatin margination and intranuclear inclusions. The most striking cytoplasmic change were the presence of perinuclear. eosinophilic inclusions at 22-36 hours post inoculation ( p. i.). Vacuolization, and granulation were also observed. Indirect immunoperoxidase ( IP ) test demonstrated the localization of influenza virus antigens in infected cells. A positive peroxidase reaction observed in the nucleus and cytoplasm were similar to those shown hematoxyline and eosin staining. H. M. I. AL-HYALIUniversity of Baghdad, College of Veterinary MedicinearticleINFLUENZA VIRUS (H4 N3)chickenCYTOPATHOGENICITYVeterinary medicineSF600-1100ENThe Iraqi Journal of Veterinary Medicine, Vol 25, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	INFLUENZA VIRUS (H4 N3) chicken CYTOPATHOGENICITY Veterinary medicine SF600-1100
spellingShingle	INFLUENZA VIRUS (H4 N3) chicken CYTOPATHOGENICITY Veterinary medicine SF600-1100 H. M. I. AL-HYALI CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)
description	Monolayer tissue cultures of chicken embryo fibroblast ( CEF ) cells infected with avian influenza virus isolate were examined by the hematoxylie and eosin (H&E) staining and indirect immunoperoxidase test for studying the cytopathogenic effect of the virus. Cytopathological changes which occurred in the uncleus of infected cells included nuclear and nucleolar hypertropy, chromatin margination and intranuclear inclusions. The most striking cytoplasmic change were the presence of perinuclear. eosinophilic inclusions at 22-36 hours post inoculation ( p. i.). Vacuolization, and granulation were also observed. Indirect immunoperoxidase ( IP ) test demonstrated the localization of influenza virus antigens in infected cells. A positive peroxidase reaction observed in the nucleus and cytoplasm were similar to those shown hematoxyline and eosin staining.
format	article
author	H. M. I. AL-HYALI
author_facet	H. M. I. AL-HYALI
author_sort	H. M. I. AL-HYALI
title	CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)
title_short	CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)
title_full	CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)
title_fullStr	CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)
title_full_unstemmed	CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)
title_sort	cytopathogenicity of influenza virus (h4 n3)
publisher	University of Baghdad, College of Veterinary Medicine
publishDate	2021
url	https://doaj.org/article/8d097a5c44d34f5a946d787ec2ac997f
work_keys_str_mv	AT hmialhyali cytopathogenicityofinfluenzavirush4n3
_version_	1718443680697679872

CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)

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