Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome

Brain diseases including Down syndrome (DS/TS21) are known to be characterized by changes in cellular metabolism. To adequately assess such metabolic changes during pathological processes and to test drugs, methods are needed that allow monitoring of these changes in real time with minimally invasiv...

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Autores principales:	Alena Kashirina, Alena Gavrina, Emil Kryukov, Vadim Elagin, Yuliya Kolesova, Alexander Artyuhov, Ekaterina Momotyuk, Vepa Abdyyev, Natalia Meshcheryakova, Elena Zagaynova, Erdem Dashinimaev, Aleksandra Kashina
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	iPSC spheroids Down syndrome metabolism NAD(P)H pH Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8d124232fe43467daa2accd9a641eaef
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spelling	oai:doaj.org-article:8d124232fe43467daa2accd9a641eaef2021-11-25T16:51:39ZEnergy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome10.3390/biomedicines91117412227-9059https://doaj.org/article/8d124232fe43467daa2accd9a641eaef2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1741https://doaj.org/toc/2227-9059Brain diseases including Down syndrome (DS/TS21) are known to be characterized by changes in cellular metabolism. To adequately assess such metabolic changes during pathological processes and to test drugs, methods are needed that allow monitoring of these changes in real time with minimally invasive effects. Thus, the aim of our work was to study the metabolic status and intracellular pH of spheroids carrying DS using fluorescence microscopy and FLIM. For metabolic analysis we measured the fluorescence intensities, fluorescence lifetimes and the contributions of the free and bound forms of NAD(P)H. For intracellular pH assay we measured the fluorescence intensities of SypHer-2 and BCECF. Data were processed with SPCImage and Fiji-ImageJ. We demonstrated the predominance of glycolysis in TS21 spheroids compared with normal karyotype (NK) spheroids. Assessment of the intracellular pH indicated a more alkaline intracellular pH in the TS21 spheroids compared to NK spheroids. Using fluorescence imaging, we performed a comprehensive comparative analysis of the metabolism and intracellular pH of TS21 spheroids and showed that fluorescence microscopy and FLIM make it possible to study living cells in 3D models in real time with minimally invasive effects.Alena KashirinaAlena GavrinaEmil KryukovVadim ElaginYuliya KolesovaAlexander ArtyuhovEkaterina MomotyukVepa AbdyyevNatalia MeshcheryakovaElena ZagaynovaErdem DashinimaevAleksandra KashinaMDPI AGarticleiPSCspheroidsDown syndromemetabolismNAD(P)HpHBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1741, p 1741 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iPSC spheroids Down syndrome metabolism NAD(P)H pH Biology (General) QH301-705.5
spellingShingle	iPSC spheroids Down syndrome metabolism NAD(P)H pH Biology (General) QH301-705.5 Alena Kashirina Alena Gavrina Emil Kryukov Vadim Elagin Yuliya Kolesova Alexander Artyuhov Ekaterina Momotyuk Vepa Abdyyev Natalia Meshcheryakova Elena Zagaynova Erdem Dashinimaev Aleksandra Kashina Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
description	Brain diseases including Down syndrome (DS/TS21) are known to be characterized by changes in cellular metabolism. To adequately assess such metabolic changes during pathological processes and to test drugs, methods are needed that allow monitoring of these changes in real time with minimally invasive effects. Thus, the aim of our work was to study the metabolic status and intracellular pH of spheroids carrying DS using fluorescence microscopy and FLIM. For metabolic analysis we measured the fluorescence intensities, fluorescence lifetimes and the contributions of the free and bound forms of NAD(P)H. For intracellular pH assay we measured the fluorescence intensities of SypHer-2 and BCECF. Data were processed with SPCImage and Fiji-ImageJ. We demonstrated the predominance of glycolysis in TS21 spheroids compared with normal karyotype (NK) spheroids. Assessment of the intracellular pH indicated a more alkaline intracellular pH in the TS21 spheroids compared to NK spheroids. Using fluorescence imaging, we performed a comprehensive comparative analysis of the metabolism and intracellular pH of TS21 spheroids and showed that fluorescence microscopy and FLIM make it possible to study living cells in 3D models in real time with minimally invasive effects.
format	article
author	Alena Kashirina Alena Gavrina Emil Kryukov Vadim Elagin Yuliya Kolesova Alexander Artyuhov Ekaterina Momotyuk Vepa Abdyyev Natalia Meshcheryakova Elena Zagaynova Erdem Dashinimaev Aleksandra Kashina
author_facet	Alena Kashirina Alena Gavrina Emil Kryukov Vadim Elagin Yuliya Kolesova Alexander Artyuhov Ekaterina Momotyuk Vepa Abdyyev Natalia Meshcheryakova Elena Zagaynova Erdem Dashinimaev Aleksandra Kashina
author_sort	Alena Kashirina
title	Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
title_short	Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
title_full	Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
title_fullStr	Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
title_full_unstemmed	Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
title_sort	energy metabolism and intracellular ph alteration in neural spheroids carrying down syndrome
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8d124232fe43467daa2accd9a641eaef
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_version_	1718412908140953600

Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome

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