Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine

According to French Paradox, red wine was famous for the potential effects on coronary heart disease (CHD), but the specific compounds against CHD were unclear. Therefore, screening and characterization of bioactive compounds from red wine was extremely necessary. In this paper, the multi-activity i...

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Autores principales:	Yuye Gao, Xie-an Yu, Bing Wang, Guo Yin, Jue Wang, Tiejie Wang, Kaishun Bi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	red wine multi-activity integrated strategy active components coronary heart disease Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/8d1272b356b7411b92674cd91fae8aef
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spelling	oai:doaj.org-article:8d1272b356b7411b92674cd91fae8aef2021-11-11T18:40:45ZBased on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine10.3390/molecules262167501420-3049https://doaj.org/article/8d1272b356b7411b92674cd91fae8aef2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6750https://doaj.org/toc/1420-3049According to French Paradox, red wine was famous for the potential effects on coronary heart disease (CHD), but the specific compounds against CHD were unclear. Therefore, screening and characterization of bioactive compounds from red wine was extremely necessary. In this paper, the multi-activity integrated strategy was developed and validated to screen, identify and quantify active compounds from red wine by using ultra high performance liquid chromatography-fraction collector (UHPLC-FC), ultra fast liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UFLC-Q-TOF/MS) and bioactive analysis. UHPLC-FC was employed to separate and collect the components from red wine, which was further identified by UFLC-Q-TOF/MS to acquire their structural information. Furthermore, the active fractions were tested for antioxidant activity, inhibitory activity against thrombin and lipase activities in vitro by the activity screening kit. As the results, there were 37 fractions had antioxidant activity, 22 fractions had thrombin inhibitory activity and 28 fractions had lipase inhibitory activity. Finally, 77 active components from red wine were screened and 12 ingredients out of them were selected for quantification based on the integration of multi-activity. Collectively, the multi-activity integrated strategy was helpful for the rapid and effective discovery of bioactive components, which provided reference for exploring the health care function of food.Yuye GaoXie-an YuBing WangGuo YinJue WangTiejie WangKaishun BiMDPI AGarticlered winemulti-activity integrated strategyactive componentscoronary heart diseaseOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6750, p 6750 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	red wine multi-activity integrated strategy active components coronary heart disease Organic chemistry QD241-441
spellingShingle	red wine multi-activity integrated strategy active components coronary heart disease Organic chemistry QD241-441 Yuye Gao Xie-an Yu Bing Wang Guo Yin Jue Wang Tiejie Wang Kaishun Bi Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine
description	According to French Paradox, red wine was famous for the potential effects on coronary heart disease (CHD), but the specific compounds against CHD were unclear. Therefore, screening and characterization of bioactive compounds from red wine was extremely necessary. In this paper, the multi-activity integrated strategy was developed and validated to screen, identify and quantify active compounds from red wine by using ultra high performance liquid chromatography-fraction collector (UHPLC-FC), ultra fast liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UFLC-Q-TOF/MS) and bioactive analysis. UHPLC-FC was employed to separate and collect the components from red wine, which was further identified by UFLC-Q-TOF/MS to acquire their structural information. Furthermore, the active fractions were tested for antioxidant activity, inhibitory activity against thrombin and lipase activities in vitro by the activity screening kit. As the results, there were 37 fractions had antioxidant activity, 22 fractions had thrombin inhibitory activity and 28 fractions had lipase inhibitory activity. Finally, 77 active components from red wine were screened and 12 ingredients out of them were selected for quantification based on the integration of multi-activity. Collectively, the multi-activity integrated strategy was helpful for the rapid and effective discovery of bioactive components, which provided reference for exploring the health care function of food.
format	article
author	Yuye Gao Xie-an Yu Bing Wang Guo Yin Jue Wang Tiejie Wang Kaishun Bi
author_facet	Yuye Gao Xie-an Yu Bing Wang Guo Yin Jue Wang Tiejie Wang Kaishun Bi
author_sort	Yuye Gao
title	Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine
title_short	Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine
title_full	Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine
title_fullStr	Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine
title_full_unstemmed	Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine
title_sort	based on multi-activity integrated strategy to screening, characterization and quantification of bioactive compounds from red wine
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8d1272b356b7411b92674cd91fae8aef
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Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine

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