Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway

There is a growing need to develop anti-inflammatory drugs to regulate inflammatory responses. An extract of <i>Huberia peruviana</i> Cogn. had the best inhibitory effect on nitric oxide (NO) production in screening process undertaken in our laboratory. However, the anti-inflammatory eff...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Seung A Kim, Chae Young Lee, Ankita Mitra, Haeyeop Kim, Byoung Young Woo, Yong Deog Hong, Jin Kyoung Noh, Dong-Keun Yi, Han Gyung Kim, Jae Youl Cho
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Huberia peruviana</i> Cogn. methanol extract NF-κB pathway anti-inflammation Src acute gastritis Botany QK1-989
Acceso en línea:	https://doaj.org/article/8d213f4bcf7f472e9d2097feb1a4d27f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8d213f4bcf7f472e9d2097feb1a4d27f
record_format	dspace
spelling	oai:doaj.org-article:8d213f4bcf7f472e9d2097feb1a4d27f2021-11-25T18:45:28ZAnti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway10.3390/plants101123352223-7747https://doaj.org/article/8d213f4bcf7f472e9d2097feb1a4d27f2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2335https://doaj.org/toc/2223-7747There is a growing need to develop anti-inflammatory drugs to regulate inflammatory responses. An extract of <i>Huberia peruviana</i> Cogn. had the best inhibitory effect on nitric oxide (NO) production in screening process undertaken in our laboratory. However, the anti-inflammatory effect <i>of Huberia peruviana</i> Cogn. methanol extract (Hp-ME) has not been studied. In this study, the anti-inflammatory effect of Hp-ME was assessed by using an NO assay, RT-PCR, luciferase reporter gene activity assay, western blotting assay, HCl/EtOH-induced acute gastritis model, and LPS-induced acute lung injury model. The phytochemical components of Hp-ME were determined through LC-MS/MS analysis. When RAW264.7 and HEK293T cells were treated with Hp-ME, NO production was decreased dose-dependently without cytotoxicity and the mRNA levels of iNOS, COX-2, and TNF-α were decreased. In a luciferase assay, the activity of transcription factors, NF-κB in TRIF or MyD88-overexpressing HEK293T cells was extremely reduced by Hp-ME. The western blotting analysis indicated that Hp-ME has anti-inflammatory effects by inhibiting the phosphorylation of Src. Hp-ME showed anti-inflammatory effects on in vivo models of HCl/EtOH-induced gastritis and LPS-induced acute lung injury. LC-MS/MS revealed that Hp-ME contains several anti-inflammatory flavonoids. The final findings of this study imply that Hp-ME could be used as an anti-inflammatory drug in several inflammatory diseases.Seung A KimChae Young LeeAnkita MitraHaeyeop KimByoung Young WooYong Deog HongJin Kyoung NohDong-Keun YiHan Gyung KimJae Youl ChoMDPI AGarticle<i>Huberia peruviana</i> Cogn.methanol extractNF-κB pathwayanti-inflammationSrcacute gastritisBotanyQK1-989ENPlants, Vol 10, Iss 2335, p 2335 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Huberia peruviana</i> Cogn. methanol extract NF-κB pathway anti-inflammation Src acute gastritis Botany QK1-989
spellingShingle	<i>Huberia peruviana</i> Cogn. methanol extract NF-κB pathway anti-inflammation Src acute gastritis Botany QK1-989 Seung A Kim Chae Young Lee Ankita Mitra Haeyeop Kim Byoung Young Woo Yong Deog Hong Jin Kyoung Noh Dong-Keun Yi Han Gyung Kim Jae Youl Cho Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway
description	There is a growing need to develop anti-inflammatory drugs to regulate inflammatory responses. An extract of <i>Huberia peruviana</i> Cogn. had the best inhibitory effect on nitric oxide (NO) production in screening process undertaken in our laboratory. However, the anti-inflammatory effect <i>of Huberia peruviana</i> Cogn. methanol extract (Hp-ME) has not been studied. In this study, the anti-inflammatory effect of Hp-ME was assessed by using an NO assay, RT-PCR, luciferase reporter gene activity assay, western blotting assay, HCl/EtOH-induced acute gastritis model, and LPS-induced acute lung injury model. The phytochemical components of Hp-ME were determined through LC-MS/MS analysis. When RAW264.7 and HEK293T cells were treated with Hp-ME, NO production was decreased dose-dependently without cytotoxicity and the mRNA levels of iNOS, COX-2, and TNF-α were decreased. In a luciferase assay, the activity of transcription factors, NF-κB in TRIF or MyD88-overexpressing HEK293T cells was extremely reduced by Hp-ME. The western blotting analysis indicated that Hp-ME has anti-inflammatory effects by inhibiting the phosphorylation of Src. Hp-ME showed anti-inflammatory effects on in vivo models of HCl/EtOH-induced gastritis and LPS-induced acute lung injury. LC-MS/MS revealed that Hp-ME contains several anti-inflammatory flavonoids. The final findings of this study imply that Hp-ME could be used as an anti-inflammatory drug in several inflammatory diseases.
format	article
author	Seung A Kim Chae Young Lee Ankita Mitra Haeyeop Kim Byoung Young Woo Yong Deog Hong Jin Kyoung Noh Dong-Keun Yi Han Gyung Kim Jae Youl Cho
author_facet	Seung A Kim Chae Young Lee Ankita Mitra Haeyeop Kim Byoung Young Woo Yong Deog Hong Jin Kyoung Noh Dong-Keun Yi Han Gyung Kim Jae Youl Cho
author_sort	Seung A Kim
title	Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway
title_short	Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway
title_full	Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway
title_fullStr	Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway
title_full_unstemmed	Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway
title_sort	anti-inflammatory effects of <i>huberia peruviana</i> cogn. methanol extract by inhibiting src activity in the nf-κb pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8d213f4bcf7f472e9d2097feb1a4d27f
work_keys_str_mv	AT seungakim antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT chaeyounglee antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT ankitamitra antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT haeyeopkim antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT byoungyoungwoo antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT yongdeoghong antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT jinkyoungnoh antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT dongkeunyi antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT hangyungkim antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway AT jaeyoulcho antiinflammatoryeffectsofihuberiaperuvianaicognmethanolextractbyinhibitingsrcactivityinthenfkbpathway
_version_	1718410724810686464

Anti-Inflammatory Effects of <i>Huberia peruviana</i> Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway

Ejemplares similares