Biosynthesis of gold nanoparticles using chloroplasts

Yi Xia Zhang1, Jun Zheng2, Guo Gao1, Yi Fei Kong1, Xiao Zhi1, Kan Wang1, Xue Qing Zhang1, Da Xiang Cui11Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute...

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Autores principales:	Zhang YX, Zheng J, Gao G, Kong YF, Zhi X, Wang K, Zhang XQ, Cui DX
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8d2792aacfad452cbb6fd41e06909fe4
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spelling	oai:doaj.org-article:8d2792aacfad452cbb6fd41e06909fe42021-12-02T00:30:23ZBiosynthesis of gold nanoparticles using chloroplasts1176-91141178-2013https://doaj.org/article/8d2792aacfad452cbb6fd41e06909fe42011-11-01T00:00:00Zhttp://www.dovepress.com/biosynthesis-of-gold-nanoparticles-using-chloroplasts-a8699https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yi Xia Zhang1, Jun Zheng2, Guo Gao1, Yi Fei Kong1, Xiao Zhi1, Kan Wang1, Xue Qing Zhang1, Da Xiang Cui11Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, 2Wheat Research Institute, Academy of Agricultural Sciences, Linfen, Shan Xi, People&#39;s Republic of ChinaAbstract: In this paper, a new method of one-pot biosynthesizing of gold nanoparticles (GNPs), using chloroplasts as reductants and stabilizers is reported. The as-prepared GNPs were characterized by ultraviolet visible spectroscopy, transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the GNPs was evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against gastric mucous cell line GES-1 and gastric cancer cell line MGC-803. Rhodamine 6G as a Raman probe was used for investigating surface-enhanced Raman spectroscopy (SERS) enhancement of GNPs. The transmission electron microscopy results indicated that the GNPs were spherical in structure and almost 20 nm in diameter. Ultraviolet visible spectroscopy exhibited an absorption peak at 545 nm. The GNPs exhibited high crystallinity, with the (111) plane as the predominant orientation, clarified by X-ray powder diffraction. In addition, a potential mechanism was proposed to interpret the formation process of GNPs, mainly based on the analysis of FTIR results. The FTIR spectrum confirmed that the GNPs were carried with N&ndash;H groups. Toxicological assays of as-prepared GNPs revealed that the green GNPs were nontoxic. SERS analysis revealed that the GNPs without any treatment could substantially enhance the Raman signals of rhodamine 6G. The Raman enhancement factor was calculated to be nearly 1010 orders of magnitude. In conclusion, the GNPs with good biocompatibility and excellent SERS effect were successfully synthesized using chloroplasts. These biogenetic GNPs have great potential for ultrasensitive detection of biomarkers in vitro and in vivo based on SERS.Keywords: green synthesis, room temperature, gold nanospheres, MTT, SERSZhang YXZheng JGao GKong YFZhi XWang KZhang XQCui DXDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2899-2906 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang YX Zheng J Gao G Kong YF Zhi X Wang K Zhang XQ Cui DX Biosynthesis of gold nanoparticles using chloroplasts
description	Yi Xia Zhang1, Jun Zheng2, Guo Gao1, Yi Fei Kong1, Xiao Zhi1, Kan Wang1, Xue Qing Zhang1, Da Xiang Cui11Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, 2Wheat Research Institute, Academy of Agricultural Sciences, Linfen, Shan Xi, People&#39;s Republic of ChinaAbstract: In this paper, a new method of one-pot biosynthesizing of gold nanoparticles (GNPs), using chloroplasts as reductants and stabilizers is reported. The as-prepared GNPs were characterized by ultraviolet visible spectroscopy, transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the GNPs was evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against gastric mucous cell line GES-1 and gastric cancer cell line MGC-803. Rhodamine 6G as a Raman probe was used for investigating surface-enhanced Raman spectroscopy (SERS) enhancement of GNPs. The transmission electron microscopy results indicated that the GNPs were spherical in structure and almost 20 nm in diameter. Ultraviolet visible spectroscopy exhibited an absorption peak at 545 nm. The GNPs exhibited high crystallinity, with the (111) plane as the predominant orientation, clarified by X-ray powder diffraction. In addition, a potential mechanism was proposed to interpret the formation process of GNPs, mainly based on the analysis of FTIR results. The FTIR spectrum confirmed that the GNPs were carried with N&ndash;H groups. Toxicological assays of as-prepared GNPs revealed that the green GNPs were nontoxic. SERS analysis revealed that the GNPs without any treatment could substantially enhance the Raman signals of rhodamine 6G. The Raman enhancement factor was calculated to be nearly 1010 orders of magnitude. In conclusion, the GNPs with good biocompatibility and excellent SERS effect were successfully synthesized using chloroplasts. These biogenetic GNPs have great potential for ultrasensitive detection of biomarkers in vitro and in vivo based on SERS.Keywords: green synthesis, room temperature, gold nanospheres, MTT, SERS
format	article
author	Zhang YX Zheng J Gao G Kong YF Zhi X Wang K Zhang XQ Cui DX
author_facet	Zhang YX Zheng J Gao G Kong YF Zhi X Wang K Zhang XQ Cui DX
author_sort	Zhang YX
title	Biosynthesis of gold nanoparticles using chloroplasts
title_short	Biosynthesis of gold nanoparticles using chloroplasts
title_full	Biosynthesis of gold nanoparticles using chloroplasts
title_fullStr	Biosynthesis of gold nanoparticles using chloroplasts
title_full_unstemmed	Biosynthesis of gold nanoparticles using chloroplasts
title_sort	biosynthesis of gold nanoparticles using chloroplasts
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/8d2792aacfad452cbb6fd41e06909fe4
work_keys_str_mv	AT zhangyx biosynthesisofgoldnanoparticlesusingchloroplasts AT zhengj biosynthesisofgoldnanoparticlesusingchloroplasts AT gaog biosynthesisofgoldnanoparticlesusingchloroplasts AT kongyf biosynthesisofgoldnanoparticlesusingchloroplasts AT zhix biosynthesisofgoldnanoparticlesusingchloroplasts AT wangk biosynthesisofgoldnanoparticlesusingchloroplasts AT zhangxq biosynthesisofgoldnanoparticlesusingchloroplasts AT cuidx biosynthesisofgoldnanoparticlesusingchloroplasts
_version_	1718403687430225920

Biosynthesis of gold nanoparticles using chloroplasts

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