Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.

Although alveolar epithelial type II cells (AECII) perform substantial roles in the maintenance of alveolar integrity, the extent of their contributions to immune defense is poorly understood. Here, we demonstrate that AECII activates alveolar macrophages (AM) functions, such as phagocytosis using a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shibichakravarthy Kannan, Huang Huang, Drew Seeger, Aaron Audet, Yaoyu Chen, Canhua Huang, Hongwei Gao, Shaoguang Li, Min Wu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d30f9b8ad984df0bf49d135a58c810d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8d30f9b8ad984df0bf49d135a58c810d
record_format	dspace
spelling	oai:doaj.org-article:8d30f9b8ad984df0bf49d135a58c810d2021-11-25T06:16:34ZAlveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.1932-620310.1371/journal.pone.0004891https://doaj.org/article/8d30f9b8ad984df0bf49d135a58c810d2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19305493/?tool=EBIhttps://doaj.org/toc/1932-6203Although alveolar epithelial type II cells (AECII) perform substantial roles in the maintenance of alveolar integrity, the extent of their contributions to immune defense is poorly understood. Here, we demonstrate that AECII activates alveolar macrophages (AM) functions, such as phagocytosis using a conditioned medium from AECII infected by P. aeruginosa. AECII-derived chemokine MCP-1, a monocyte chemoattractant protein, was identified as a main factor in enhancing AM function. We proposed that the enhanced immune potency of AECII may play a critical role in alleviation of bacterial propagation and pneumonia. The ability of phagocytosis and superoxide release by AM was reduced by MCP-1 neutralizing antibodies. Furthermore, MCP-1(-/-) mice showed an increased bacterial burden under PAO1 and PAK infection vs. wt littermates. AM from MCP-1(-/-) mice also demonstrated less superoxide and impaired phagocytosis over the controls. In addition, AECII conditioned medium increased the host defense of airway in MCP-1(-/-) mice through the activation of AM function. Mechanistically, we found that Lyn mediated NFkappaB activation led to increased gene expression and secretion of MCP-1. Consequently Lyn(-/-) mice had reduced MCP-1 secretion and resulted in a decrease in superoxide and phagocytosis by AM. Collectively, our data indicate that AECII may serve as an immune booster for fighting bacterial infections, particularly in severe immunocompromised conditions.Shibichakravarthy KannanHuang HuangDrew SeegerAaron AudetYaoyu ChenCanhua HuangHongwei GaoShaoguang LiMin WuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4891 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shibichakravarthy Kannan Huang Huang Drew Seeger Aaron Audet Yaoyu Chen Canhua Huang Hongwei Gao Shaoguang Li Min Wu Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
description	Although alveolar epithelial type II cells (AECII) perform substantial roles in the maintenance of alveolar integrity, the extent of their contributions to immune defense is poorly understood. Here, we demonstrate that AECII activates alveolar macrophages (AM) functions, such as phagocytosis using a conditioned medium from AECII infected by P. aeruginosa. AECII-derived chemokine MCP-1, a monocyte chemoattractant protein, was identified as a main factor in enhancing AM function. We proposed that the enhanced immune potency of AECII may play a critical role in alleviation of bacterial propagation and pneumonia. The ability of phagocytosis and superoxide release by AM was reduced by MCP-1 neutralizing antibodies. Furthermore, MCP-1(-/-) mice showed an increased bacterial burden under PAO1 and PAK infection vs. wt littermates. AM from MCP-1(-/-) mice also demonstrated less superoxide and impaired phagocytosis over the controls. In addition, AECII conditioned medium increased the host defense of airway in MCP-1(-/-) mice through the activation of AM function. Mechanistically, we found that Lyn mediated NFkappaB activation led to increased gene expression and secretion of MCP-1. Consequently Lyn(-/-) mice had reduced MCP-1 secretion and resulted in a decrease in superoxide and phagocytosis by AM. Collectively, our data indicate that AECII may serve as an immune booster for fighting bacterial infections, particularly in severe immunocompromised conditions.
format	article
author	Shibichakravarthy Kannan Huang Huang Drew Seeger Aaron Audet Yaoyu Chen Canhua Huang Hongwei Gao Shaoguang Li Min Wu
author_facet	Shibichakravarthy Kannan Huang Huang Drew Seeger Aaron Audet Yaoyu Chen Canhua Huang Hongwei Gao Shaoguang Li Min Wu
author_sort	Shibichakravarthy Kannan
title	Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
title_short	Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
title_full	Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
title_fullStr	Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
title_full_unstemmed	Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
title_sort	alveolar epithelial type ii cells activate alveolar macrophages and mitigate p. aeruginosa infection.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/8d30f9b8ad984df0bf49d135a58c810d
work_keys_str_mv	AT shibichakravarthykannan alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT huanghuang alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT drewseeger alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT aaronaudet alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT yaoyuchen alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT canhuahuang alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT hongweigao alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT shaoguangli alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection AT minwu alveolarepithelialtypeiicellsactivatealveolarmacrophagesandmitigatepaeruginosainfection
_version_	1718413995778506752

Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.

Ejemplares similares