Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus

Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm forma...

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Autores principales:	Hellen Braga Martins Oliveira, Nathan das Neves Selis, Beatriz Almeida Sampaio, Manoel Neres Santos Júnior, Suzi Pacheco de Carvalho, Jéssica Bomfim de Almeida, Palloma Porto Almeida, Icaro Bonyek Santos da Silva, Caline Novais Teixeira Oliveira, Thamara Louisy Santos Brito, Letícia de Oliveira da Silva, Mariana Morais Teixeira, Hanna Izadora Laís Novaes Coelho, Camila Dutra Barbosa, Yasmin Monara Ferreira de Sousa Andrade, Rafaela de Souza Bittencourt, Jully Chayra Santos Viana, Guilherme Barreto Campos, Jorge Timenetsky, Ana Paula T. Uetanabaro, Regiane Yatsuda, Lucas Miranda Marques
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d32fb72134345a698de8ec7e70437c0
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spelling	oai:doaj.org-article:8d32fb72134345a698de8ec7e70437c02021-12-02T19:06:40ZCitral modulates virulence factors in methicillin-resistant Staphylococcus aureus10.1038/s41598-021-95971-y2045-2322https://doaj.org/article/8d32fb72134345a698de8ec7e70437c02021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95971-yhttps://doaj.org/toc/2045-2322Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.Hellen Braga Martins OliveiraNathan das Neves SelisBeatriz Almeida SampaioManoel Neres Santos JúniorSuzi Pacheco de CarvalhoJéssica Bomfim de AlmeidaPalloma Porto AlmeidaIcaro Bonyek Santos da SilvaCaline Novais Teixeira OliveiraThamara Louisy Santos BritoLetícia de Oliveira da SilvaMariana Morais TeixeiraHanna Izadora Laís Novaes CoelhoCamila Dutra BarbosaYasmin Monara Ferreira de Sousa AndradeRafaela de Souza BittencourtJully Chayra Santos VianaGuilherme Barreto CamposJorge TimenetskyAna Paula T. UetanabaroRegiane YatsudaLucas Miranda MarquesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hellen Braga Martins Oliveira Nathan das Neves Selis Beatriz Almeida Sampaio Manoel Neres Santos Júnior Suzi Pacheco de Carvalho Jéssica Bomfim de Almeida Palloma Porto Almeida Icaro Bonyek Santos da Silva Caline Novais Teixeira Oliveira Thamara Louisy Santos Brito Letícia de Oliveira da Silva Mariana Morais Teixeira Hanna Izadora Laís Novaes Coelho Camila Dutra Barbosa Yasmin Monara Ferreira de Sousa Andrade Rafaela de Souza Bittencourt Jully Chayra Santos Viana Guilherme Barreto Campos Jorge Timenetsky Ana Paula T. Uetanabaro Regiane Yatsuda Lucas Miranda Marques Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
description	Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.
format	article
author	Hellen Braga Martins Oliveira Nathan das Neves Selis Beatriz Almeida Sampaio Manoel Neres Santos Júnior Suzi Pacheco de Carvalho Jéssica Bomfim de Almeida Palloma Porto Almeida Icaro Bonyek Santos da Silva Caline Novais Teixeira Oliveira Thamara Louisy Santos Brito Letícia de Oliveira da Silva Mariana Morais Teixeira Hanna Izadora Laís Novaes Coelho Camila Dutra Barbosa Yasmin Monara Ferreira de Sousa Andrade Rafaela de Souza Bittencourt Jully Chayra Santos Viana Guilherme Barreto Campos Jorge Timenetsky Ana Paula T. Uetanabaro Regiane Yatsuda Lucas Miranda Marques
author_facet	Hellen Braga Martins Oliveira Nathan das Neves Selis Beatriz Almeida Sampaio Manoel Neres Santos Júnior Suzi Pacheco de Carvalho Jéssica Bomfim de Almeida Palloma Porto Almeida Icaro Bonyek Santos da Silva Caline Novais Teixeira Oliveira Thamara Louisy Santos Brito Letícia de Oliveira da Silva Mariana Morais Teixeira Hanna Izadora Laís Novaes Coelho Camila Dutra Barbosa Yasmin Monara Ferreira de Sousa Andrade Rafaela de Souza Bittencourt Jully Chayra Santos Viana Guilherme Barreto Campos Jorge Timenetsky Ana Paula T. Uetanabaro Regiane Yatsuda Lucas Miranda Marques
author_sort	Hellen Braga Martins Oliveira
title	Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
title_short	Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
title_full	Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
title_fullStr	Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
title_full_unstemmed	Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
title_sort	citral modulates virulence factors in methicillin-resistant staphylococcus aureus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8d32fb72134345a698de8ec7e70437c0
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Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus

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