Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies

Hanmei Bao,1,2 Qing Zhang,2,3 Hui Xu,1,2 Zhao Yan1,2 1Department of Clinical Pharmacology, 2Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, 3Department of Hematology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People’s...

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Autores principales:	Bao H, Zhang Q, Xu H, Yan Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	drug delivery cancer treatment gold nanoparticle particle size Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8d33df5a5b624537b753540caf5ff408
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spelling	oai:doaj.org-article:8d33df5a5b624537b753540caf5ff4082021-12-02T07:15:23ZEffects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies1178-2013https://doaj.org/article/8d33df5a5b624537b753540caf5ff4082016-03-01T00:00:00Zhttps://www.dovepress.com/effects-of-nanoparticle-size-on-antitumor-activity-of-10-hydroxycampto-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hanmei Bao,1,2 Qing Zhang,2,3 Hui Xu,1,2 Zhao Yan1,2 1Department of Clinical Pharmacology, 2Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, 3Department of Hematology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People’s Republic of China Abstract: Gold nanoparticles (AuNPs) have emerged as a promising anticancer drug delivery scaffold. However, some controversial points still require further investigation before clinical use. A complete understanding of how animal cells interact with drug-conjugated AuNPs of well-defined sizes remains poorly understood. In this study, we prepared a series of 10-hydroxycamptothecin (HCPT)-AuNP conjugates of different sizes and compared their cytotoxic effect in vitro and antitumor effect in vivo. Transmission electron micrographs showed that the NPs had a round, regular shape with a mean diameter of ~10, 25, and 50 nm. An in vitro drug release study showed that HCPT was continuously released for 120 hours. HCPT-AuNPs showed greater cytotoxic effects on the MDA-MB-231 cell line compared with an equal dose of free HCPT. Notably, HCPT-AuNPs of an average diameter of 50 nm (HCPT-AuNPs-50) had the greatest effect. Furthermore, administration of HCPT-AuNPs-50 showed the most tumor-suppressing activity against MDA-MB-231 tumor in mice among all treatment groups. The results indicate that AuNPs not only act as a carrier but also play an active role in mediating biological effects. This work gives important insights into the design of nanoscale delivery and therapeutic systems. Keywords: drug delivery, cancer treatment, drug-containing nanocomposites, particle sizeBao HZhang QXu HYan ZDove Medical Pressarticledrug deliverycancer treatmentgold nanoparticleparticle sizeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 929-940 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	drug delivery cancer treatment gold nanoparticle particle size Medicine (General) R5-920
spellingShingle	drug delivery cancer treatment gold nanoparticle particle size Medicine (General) R5-920 Bao H Zhang Q Xu H Yan Z Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies
description	Hanmei Bao,1,2 Qing Zhang,2,3 Hui Xu,1,2 Zhao Yan1,2 1Department of Clinical Pharmacology, 2Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, 3Department of Hematology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People’s Republic of China Abstract: Gold nanoparticles (AuNPs) have emerged as a promising anticancer drug delivery scaffold. However, some controversial points still require further investigation before clinical use. A complete understanding of how animal cells interact with drug-conjugated AuNPs of well-defined sizes remains poorly understood. In this study, we prepared a series of 10-hydroxycamptothecin (HCPT)-AuNP conjugates of different sizes and compared their cytotoxic effect in vitro and antitumor effect in vivo. Transmission electron micrographs showed that the NPs had a round, regular shape with a mean diameter of ~10, 25, and 50 nm. An in vitro drug release study showed that HCPT was continuously released for 120 hours. HCPT-AuNPs showed greater cytotoxic effects on the MDA-MB-231 cell line compared with an equal dose of free HCPT. Notably, HCPT-AuNPs of an average diameter of 50 nm (HCPT-AuNPs-50) had the greatest effect. Furthermore, administration of HCPT-AuNPs-50 showed the most tumor-suppressing activity against MDA-MB-231 tumor in mice among all treatment groups. The results indicate that AuNPs not only act as a carrier but also play an active role in mediating biological effects. This work gives important insights into the design of nanoscale delivery and therapeutic systems. Keywords: drug delivery, cancer treatment, drug-containing nanocomposites, particle size
format	article
author	Bao H Zhang Q Xu H Yan Z
author_facet	Bao H Zhang Q Xu H Yan Z
author_sort	Bao H
title	Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies
title_short	Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies
title_full	Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies
title_fullStr	Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies
title_full_unstemmed	Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies
title_sort	effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/8d33df5a5b624537b753540caf5ff408
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Effects of nanoparticle size on antitumor activity of 10-hydroxycamptothecin-conjugated gold nanoparticles: in vitro and in vivo studies

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