Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip

Summary: Despite colorectal cancer’s (CRC) prevalence, its progression is not well understood. The microfluidic organ-on-chip (OOC) model described herein recreates the epithelial-endothelial tissue-tissue interface, fluid flow, and mechanical forces that exist in vivo, making it an attractive model...

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Autores principales:	Carly Strelez, Kimya Ghaffarian, Shannon M. Mumenthaler
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/8d3bc2e5db42468483276425f99b3681
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spelling	oai:doaj.org-article:8d3bc2e5db42468483276425f99b36812021-12-04T04:35:59ZMultiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip2666-166710.1016/j.xpro.2021.100984https://doaj.org/article/8d3bc2e5db42468483276425f99b36812021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006900https://doaj.org/toc/2666-1667Summary: Despite colorectal cancer’s (CRC) prevalence, its progression is not well understood. The microfluidic organ-on-chip (OOC) model described herein recreates the epithelial-endothelial tissue-tissue interface, fluid flow, and mechanical forces that exist in vivo, making it an attractive model to understand and ultimately disrupt CRC intravasation. This protocol provides step-by-step details for tumor cell seeding to create a CRC-on-chip model, chip effluent collection and analysis, and on-chip imaging to monitor tumor cell invasion within a more physiologically relevant microenvironment.For complete details on the use and execution of this protocol, please refer to Strelez et al. (2021).Carly StrelezKimya GhaffarianShannon M. MumenthalerElsevierarticleScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100984- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science (General) Q1-390
spellingShingle	Science (General) Q1-390 Carly Strelez Kimya Ghaffarian Shannon M. Mumenthaler Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip
description	Summary: Despite colorectal cancer’s (CRC) prevalence, its progression is not well understood. The microfluidic organ-on-chip (OOC) model described herein recreates the epithelial-endothelial tissue-tissue interface, fluid flow, and mechanical forces that exist in vivo, making it an attractive model to understand and ultimately disrupt CRC intravasation. This protocol provides step-by-step details for tumor cell seeding to create a CRC-on-chip model, chip effluent collection and analysis, and on-chip imaging to monitor tumor cell invasion within a more physiologically relevant microenvironment.For complete details on the use and execution of this protocol, please refer to Strelez et al. (2021).
format	article
author	Carly Strelez Kimya Ghaffarian Shannon M. Mumenthaler
author_facet	Carly Strelez Kimya Ghaffarian Shannon M. Mumenthaler
author_sort	Carly Strelez
title	Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip
title_short	Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip
title_full	Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip
title_fullStr	Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip
title_full_unstemmed	Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip
title_sort	multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8d3bc2e5db42468483276425f99b3681
work_keys_str_mv	AT carlystrelez multiplexedimagingandeffluentanalysistomonitorcancercellintravasationusingacolorectalcanceronchip AT kimyaghaffarian multiplexedimagingandeffluentanalysistomonitorcancercellintravasationusingacolorectalcanceronchip AT shannonmmumenthaler multiplexedimagingandeffluentanalysistomonitorcancercellintravasationusingacolorectalcanceronchip
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Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip

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