The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis

BACKGROUND AND OBJECTIVE: Antibody production against to protective antigen (PA) can be helpful in immunotherapy and anthrax treatment. The carboxyl terminal part of PA has the most important playing in immune system induction. The objective of this study is cloning and recombinant expression of car...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Keyhan AH, Tahmasbpour Marzony E, Farhadi N, Kamali M
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2011
Materias:	bacillus anthracis pa protein cloning anthrax Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8d3d00619f124d7a97c56e7de44193c2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8d3d00619f124d7a97c56e7de44193c2
record_format	dspace
spelling	oai:doaj.org-article:8d3d00619f124d7a97c56e7de44193c22021-11-10T08:56:48ZThe Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis1561-41072251-7170https://doaj.org/article/8d3d00619f124d7a97c56e7de44193c22011-09-01T00:00:00Zhttp://jbums.org/article-1-3867-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Antibody production against to protective antigen (PA) can be helpful in immunotherapy and anthrax treatment. The carboxyl terminal part of PA has the most important playing in immune system induction. The objective of this study is cloning and recombinant expression of carboxyl site of protective protein for antibody production.METHODS: In this experimental study after DNA extraction from Bacillus anthracis, the presence of PA gene on bacterial chromosome was confirmed by PCR method. The site of carboxyl terminal from PA protein amplified by PCR method, then PCR productions and plasmid were cut out by BamH I and Hind III restriction enzymes. PCR production and plasmid transformed into E. coli BL21 (DE3). Clones containing gene of interest was determined by PCR reaction, enzyme digestion and sequencing. Moreover, the production of recombinant proteins was confirmed by SDS-PAGE and western methods.FINDINGS: The sequence of carboxyl terminal part of PA was confirmed by sequencing, PCR and enzymatic digestion method which suggestion to intended gene cloning in E. coli BL21 (DE3). SDS-PAGE and western blotting confirmed the production of recombinant protein with 20 KD in molecular weight.CONCLUSION: According to the results of this study, this recombinant protein can be produced in high levels by this method, which opens a new window for vaccine and monoclonal antibody production against the intended disease.Keyhan AH, Tahmasbpour Marzony E, Farhadi N, Kamali MBabol University of Medical Sciencesarticlebacillus anthracispa proteincloninganthraxMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 13, Iss 5, Pp 7-14 (2011)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	bacillus anthracis pa protein cloning anthrax Medicine R Medicine (General) R5-920
spellingShingle	bacillus anthracis pa protein cloning anthrax Medicine R Medicine (General) R5-920 Keyhan AH, Tahmasbpour Marzony E, Farhadi N, Kamali M The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis
description	BACKGROUND AND OBJECTIVE: Antibody production against to protective antigen (PA) can be helpful in immunotherapy and anthrax treatment. The carboxyl terminal part of PA has the most important playing in immune system induction. The objective of this study is cloning and recombinant expression of carboxyl site of protective protein for antibody production.METHODS: In this experimental study after DNA extraction from Bacillus anthracis, the presence of PA gene on bacterial chromosome was confirmed by PCR method. The site of carboxyl terminal from PA protein amplified by PCR method, then PCR productions and plasmid were cut out by BamH I and Hind III restriction enzymes. PCR production and plasmid transformed into E. coli BL21 (DE3). Clones containing gene of interest was determined by PCR reaction, enzyme digestion and sequencing. Moreover, the production of recombinant proteins was confirmed by SDS-PAGE and western methods.FINDINGS: The sequence of carboxyl terminal part of PA was confirmed by sequencing, PCR and enzymatic digestion method which suggestion to intended gene cloning in E. coli BL21 (DE3). SDS-PAGE and western blotting confirmed the production of recombinant protein with 20 KD in molecular weight.CONCLUSION: According to the results of this study, this recombinant protein can be produced in high levels by this method, which opens a new window for vaccine and monoclonal antibody production against the intended disease.
format	article
author	Keyhan AH, Tahmasbpour Marzony E, Farhadi N, Kamali M
author_facet	Keyhan AH, Tahmasbpour Marzony E, Farhadi N, Kamali M
author_sort	Keyhan AH, Tahmasbpour Marzony E, Farhadi N, Kamali M
title	The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis
title_short	The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis
title_full	The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis
title_fullStr	The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis
title_full_unstemmed	The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis
title_sort	cloning and expression of carboxyl terminal part of protective antigen from bacillus anthracis
publisher	Babol University of Medical Sciences
publishDate	2011
url	https://doaj.org/article/8d3d00619f124d7a97c56e7de44193c2
work_keys_str_mv	AT keyhanahtahmasbpourmarzonyefarhadinkamalim thecloningandexpressionofcarboxylterminalpartofprotectiveantigenfrombacillusanthracis AT keyhanahtahmasbpourmarzonyefarhadinkamalim cloningandexpressionofcarboxylterminalpartofprotectiveantigenfrombacillusanthracis
_version_	1718440304513646592

The Cloning and Expression of Carboxyl Terminal Part of Protective Antigen from Bacillus Anthracis

Ejemplares similares