Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2

Abstract This study evaluated the bone regeneration capacity and mechanical properties of block-type hydroxyapatite (HA)/tricalcium phosphate (TCP) scaffolds in response to different concentrations of polydeoxyribonucleotide (PDRN) and recombinant human bone morphogenic protein 2 (rhBMP-2). Thirty-t...

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Autores principales:	Ho-Kyung Lim, Yeh-Jin Kwon, Seok-Jin Hong, Hyo-Geun Choi, Sung-Min Chung, Byoung-Eun Yang, Jong-Ho Lee, Soo-Hwan Byun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d420558c45e4573b89e51fb85c45c9b
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spelling	oai:doaj.org-article:8d420558c45e4573b89e51fb85c45c9b2021-12-02T17:51:21ZBone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-210.1038/s41598-021-91147-w2045-2322https://doaj.org/article/8d420558c45e4573b89e51fb85c45c9b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91147-whttps://doaj.org/toc/2045-2322Abstract This study evaluated the bone regeneration capacity and mechanical properties of block-type hydroxyapatite (HA)/tricalcium phosphate (TCP) scaffolds in response to different concentrations of polydeoxyribonucleotide (PDRN) and recombinant human bone morphogenic protein 2 (rhBMP-2). Thirty-two male white rabbits were used as a model of calvarial bone defect and classified into eight groups according to type and concentration of growth factor administered, viz., control group (only HA/TCP scaffold), scaffold + PDRN (0.1, 1, 5, and 10 mg/mL each) and scaffold + rhBMP-2 (0.01, 0.05, and 0.1 mg/mL each). The specimens were evaluated using histomorphometric and radiological analyses. Histomorphometric analyses indicated that the administration of PDRN did not increase bone formation. However, significant increases in bone formation were observed with the administration of rhBMP-2 at 0.05 and 0.10 mg/mL on week 8 compared to the control (p < 0.05). Radiological analyses revealed a significant increase in bone formation at week 8 with the administration of PDRN at 5 mg/mL and 10 mg/mL, and rhBMP-2 at 0.05 or 0.10 mg/mL compared to the control (p < 0.05). Our findings show that block-type HA/TCP scaffolds possess sufficient mechanical strength and bone regeneration capacity when used with optimal concentrations of growth factors.Ho-Kyung LimYeh-Jin KwonSeok-Jin HongHyo-Geun ChoiSung-Min ChungByoung-Eun YangJong-Ho LeeSoo-Hwan ByunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ho-Kyung Lim Yeh-Jin Kwon Seok-Jin Hong Hyo-Geun Choi Sung-Min Chung Byoung-Eun Yang Jong-Ho Lee Soo-Hwan Byun Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2
description	Abstract This study evaluated the bone regeneration capacity and mechanical properties of block-type hydroxyapatite (HA)/tricalcium phosphate (TCP) scaffolds in response to different concentrations of polydeoxyribonucleotide (PDRN) and recombinant human bone morphogenic protein 2 (rhBMP-2). Thirty-two male white rabbits were used as a model of calvarial bone defect and classified into eight groups according to type and concentration of growth factor administered, viz., control group (only HA/TCP scaffold), scaffold + PDRN (0.1, 1, 5, and 10 mg/mL each) and scaffold + rhBMP-2 (0.01, 0.05, and 0.1 mg/mL each). The specimens were evaluated using histomorphometric and radiological analyses. Histomorphometric analyses indicated that the administration of PDRN did not increase bone formation. However, significant increases in bone formation were observed with the administration of rhBMP-2 at 0.05 and 0.10 mg/mL on week 8 compared to the control (p < 0.05). Radiological analyses revealed a significant increase in bone formation at week 8 with the administration of PDRN at 5 mg/mL and 10 mg/mL, and rhBMP-2 at 0.05 or 0.10 mg/mL compared to the control (p < 0.05). Our findings show that block-type HA/TCP scaffolds possess sufficient mechanical strength and bone regeneration capacity when used with optimal concentrations of growth factors.
format	article
author	Ho-Kyung Lim Yeh-Jin Kwon Seok-Jin Hong Hyo-Geun Choi Sung-Min Chung Byoung-Eun Yang Jong-Ho Lee Soo-Hwan Byun
author_facet	Ho-Kyung Lim Yeh-Jin Kwon Seok-Jin Hong Hyo-Geun Choi Sung-Min Chung Byoung-Eun Yang Jong-Ho Lee Soo-Hwan Byun
author_sort	Ho-Kyung Lim
title	Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2
title_short	Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2
title_full	Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2
title_fullStr	Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2
title_full_unstemmed	Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2
title_sort	bone regeneration in ceramic scaffolds with variable concentrations of pdrn and rhbmp-2
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8d420558c45e4573b89e51fb85c45c9b
work_keys_str_mv	AT hokyunglim boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2 AT yehjinkwon boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2 AT seokjinhong boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2 AT hyogeunchoi boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2 AT sungminchung boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2 AT byoungeunyang boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2 AT jongholee boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2 AT soohwanbyun boneregenerationinceramicscaffoldswithvariableconcentrationsofpdrnandrhbmp2
_version_	1718379297804124160

Bone regeneration in ceramic scaffolds with variable concentrations of PDRN and rhBMP-2

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