The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis

Abstract ChHP1BP3, a chromatin complex-related protein known with dynamic features, was identified as a ChHsp70 promoter-associated factor in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. Direct interaction between purified ChHP1BP3 and the ChHsp70 promoter r...

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Autores principales:	Delin Xu, Qin Yang, Miao Cui, Qizhong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d456a6ef71e4311a528096b58e40664
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spelling	oai:doaj.org-article:8d456a6ef71e4311a528096b58e406642021-12-02T16:06:51ZThe novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis10.1038/s41598-017-01573-y2045-2322https://doaj.org/article/8d456a6ef71e4311a528096b58e406642017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01573-yhttps://doaj.org/toc/2045-2322Abstract ChHP1BP3, a chromatin complex-related protein known with dynamic features, was identified as a ChHsp70 promoter-associated factor in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. Direct interaction between purified ChHP1BP3 and the ChHsp70 promoter region was demonstrated using EMSA. ChHp1bp3 depletion led to clear enhancements in ChHsp70 mRNA expression in C. hongkongensis hemocytes. However, ChHp1bp3 overexpression in heterologous HEK293T cells correlated with fluctuations in ChHsp70 transcription. Quantitative RT-PCR analysis showed that both ChHsp70 and ChHp1bp3 transcription were responsive to external physical/chemical stresses by heat, CdCl2 and NP. This indicated a plausible correlation between ChHsp70 and ChHp1bp3 in the stress-induced genetic regulatory pathway. While, the distinctive ChHp1bp3 expression patterns upon physical and chemical stresses suggest that the mechanisms that mediate ChHp1bp3 induction might be stress-specific. This study discovered a novel role for HP1BP3 as a negative regulator in controlling Hsp70 transcription in C. hongkongensis, and contributed to better understanding the complex regulatory mechanisms governing Hsp70 transcription.Delin XuQin YangMiao CuiQizhong ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Delin Xu Qin Yang Miao Cui Qizhong Zhang The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
description	Abstract ChHP1BP3, a chromatin complex-related protein known with dynamic features, was identified as a ChHsp70 promoter-associated factor in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. Direct interaction between purified ChHP1BP3 and the ChHsp70 promoter region was demonstrated using EMSA. ChHp1bp3 depletion led to clear enhancements in ChHsp70 mRNA expression in C. hongkongensis hemocytes. However, ChHp1bp3 overexpression in heterologous HEK293T cells correlated with fluctuations in ChHsp70 transcription. Quantitative RT-PCR analysis showed that both ChHsp70 and ChHp1bp3 transcription were responsive to external physical/chemical stresses by heat, CdCl2 and NP. This indicated a plausible correlation between ChHsp70 and ChHp1bp3 in the stress-induced genetic regulatory pathway. While, the distinctive ChHp1bp3 expression patterns upon physical and chemical stresses suggest that the mechanisms that mediate ChHp1bp3 induction might be stress-specific. This study discovered a novel role for HP1BP3 as a negative regulator in controlling Hsp70 transcription in C. hongkongensis, and contributed to better understanding the complex regulatory mechanisms governing Hsp70 transcription.
format	article
author	Delin Xu Qin Yang Miao Cui Qizhong Zhang
author_facet	Delin Xu Qin Yang Miao Cui Qizhong Zhang
author_sort	Delin Xu
title	The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
title_short	The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
title_full	The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
title_fullStr	The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
title_full_unstemmed	The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
title_sort	novel transcriptional factor hp1bp3 negatively regulates hsp70 transcription in crassostrea hongkongensis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8d456a6ef71e4311a528096b58e40664
work_keys_str_mv	AT delinxu thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT qinyang thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT miaocui thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT qizhongzhang thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT delinxu noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT qinyang noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT miaocui noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT qizhongzhang noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis
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The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis

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