Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue

Abstract Several pathologic conditions of the heart lead to cardiac structural remodelling. Given the high density and the opaque nature of the myocardium, deep three dimensional (3D) imaging is difficult to achieve and structural analysis of pathological myocardial structure is often limited to two...

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Autores principales:	Filippo Perbellini, Alan K. L. Liu, Samuel A. Watson, Ifigeneia Bardi, Stephen M. Rothery, Cesare M. Terracciano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d48eaeaeee24bf3b3d109a86746c56c
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spelling	oai:doaj.org-article:8d48eaeaeee24bf3b3d109a86746c56c2021-12-02T12:32:58ZFree-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue10.1038/s41598-017-05406-w2045-2322https://doaj.org/article/8d48eaeaeee24bf3b3d109a86746c56c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05406-whttps://doaj.org/toc/2045-2322Abstract Several pathologic conditions of the heart lead to cardiac structural remodelling. Given the high density and the opaque nature of the myocardium, deep three dimensional (3D) imaging is difficult to achieve and structural analysis of pathological myocardial structure is often limited to two dimensional images and of thin myocardial sections. Efficient methods to obtain optical clearing of the tissue for 3D visualisation are therefore needed. Here we describe a rapid, simple and versatile Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) protocol specifically designed for cardiac tissue. With this method 3D information regarding collagen content, collagen localization and distribution could be easily obtained across a whole 300 µm-thick myocardial slice. FASTClear does not induce structural or microstructural distortion and it can be combined with immunostaining to identify the micro- and macrovascular networks. In summary, we have obtained decolorized myocardial tissue suitable for high resolution 3D imaging, with implications for the study of complex cardiac tissue structure and its changes during pathology.Filippo PerbelliniAlan K. L. LiuSamuel A. WatsonIfigeneia BardiStephen M. RotheryCesare M. TerraccianoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Filippo Perbellini Alan K. L. Liu Samuel A. Watson Ifigeneia Bardi Stephen M. Rothery Cesare M. Terracciano Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue
description	Abstract Several pathologic conditions of the heart lead to cardiac structural remodelling. Given the high density and the opaque nature of the myocardium, deep three dimensional (3D) imaging is difficult to achieve and structural analysis of pathological myocardial structure is often limited to two dimensional images and of thin myocardial sections. Efficient methods to obtain optical clearing of the tissue for 3D visualisation are therefore needed. Here we describe a rapid, simple and versatile Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) protocol specifically designed for cardiac tissue. With this method 3D information regarding collagen content, collagen localization and distribution could be easily obtained across a whole 300 µm-thick myocardial slice. FASTClear does not induce structural or microstructural distortion and it can be combined with immunostaining to identify the micro- and macrovascular networks. In summary, we have obtained decolorized myocardial tissue suitable for high resolution 3D imaging, with implications for the study of complex cardiac tissue structure and its changes during pathology.
format	article
author	Filippo Perbellini Alan K. L. Liu Samuel A. Watson Ifigeneia Bardi Stephen M. Rothery Cesare M. Terracciano
author_facet	Filippo Perbellini Alan K. L. Liu Samuel A. Watson Ifigeneia Bardi Stephen M. Rothery Cesare M. Terracciano
author_sort	Filippo Perbellini
title	Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue
title_short	Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue
title_full	Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue
title_fullStr	Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue
title_full_unstemmed	Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue
title_sort	free-of-acrylamide sds-based tissue clearing (fastclear) for three dimensional visualization of myocardial tissue
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8d48eaeaeee24bf3b3d109a86746c56c
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Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue

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