Microinjection of pruritogens in NGF-sensitized human skin

Abstract Single intradermal injections of nerve growth factor (NGF) evoke prolonged but temporally distinct sensitization patterns to somatosensory stimuli. Focal administration of the non-histaminergic pruritogen cowhage but not histamine resulted in elevated itch at day 21 after NGF administration...

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Autores principales:	Hans Jürgen Solinski, Roman Rukwied, Martin Schmelz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d4c5dba13f946068dab5b501ebb67c2
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spelling	oai:doaj.org-article:8d4c5dba13f946068dab5b501ebb67c22021-11-08T10:49:13ZMicroinjection of pruritogens in NGF-sensitized human skin10.1038/s41598-021-00935-x2045-2322https://doaj.org/article/8d4c5dba13f946068dab5b501ebb67c22021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00935-xhttps://doaj.org/toc/2045-2322Abstract Single intradermal injections of nerve growth factor (NGF) evoke prolonged but temporally distinct sensitization patterns to somatosensory stimuli. Focal administration of the non-histaminergic pruritogen cowhage but not histamine resulted in elevated itch at day 21 after NGF administration. Here, we injected bovine adrenal medulla peptide 8–22 (BAM8–22), β-alanine (β-ALA) and endothelin-1 (ET-1) into NGF-treated skin of 11 healthy volunteers and investigated the corresponding itch/pain and flare reactions. β-ALA was the weakest pruritogen, while BAM8–22 and ET-1 were equally potent as histamine. NGF did not sensitize itch or flare reactions induced by any compound, but injection and evoked pain were increased at day 21 and 49. The involvement of histamine H1 receptors in itch was explored in eight subjects after oral cetirizine. ET-1-induced itch and flare were significantly reduced. BAM8–22 and β-ALA itch were not affected, but flare responses after BAM8–22 reduced by 50%. The results indicate that a single NGF injection does not sensitize for experimentally induced itch but increases pain upon pruritogen injection. In healthy humans, pruritic and algetic processing appear differentially regulated by NGF. However, in patients suffering chronic itch, prolonged elevation of NGF-levels under inflammatory conditions may contribute to elevated itch.Hans Jürgen SolinskiRoman RukwiedMartin SchmelzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hans Jürgen Solinski Roman Rukwied Martin Schmelz Microinjection of pruritogens in NGF-sensitized human skin
description	Abstract Single intradermal injections of nerve growth factor (NGF) evoke prolonged but temporally distinct sensitization patterns to somatosensory stimuli. Focal administration of the non-histaminergic pruritogen cowhage but not histamine resulted in elevated itch at day 21 after NGF administration. Here, we injected bovine adrenal medulla peptide 8–22 (BAM8–22), β-alanine (β-ALA) and endothelin-1 (ET-1) into NGF-treated skin of 11 healthy volunteers and investigated the corresponding itch/pain and flare reactions. β-ALA was the weakest pruritogen, while BAM8–22 and ET-1 were equally potent as histamine. NGF did not sensitize itch or flare reactions induced by any compound, but injection and evoked pain were increased at day 21 and 49. The involvement of histamine H1 receptors in itch was explored in eight subjects after oral cetirizine. ET-1-induced itch and flare were significantly reduced. BAM8–22 and β-ALA itch were not affected, but flare responses after BAM8–22 reduced by 50%. The results indicate that a single NGF injection does not sensitize for experimentally induced itch but increases pain upon pruritogen injection. In healthy humans, pruritic and algetic processing appear differentially regulated by NGF. However, in patients suffering chronic itch, prolonged elevation of NGF-levels under inflammatory conditions may contribute to elevated itch.
format	article
author	Hans Jürgen Solinski Roman Rukwied Martin Schmelz
author_facet	Hans Jürgen Solinski Roman Rukwied Martin Schmelz
author_sort	Hans Jürgen Solinski
title	Microinjection of pruritogens in NGF-sensitized human skin
title_short	Microinjection of pruritogens in NGF-sensitized human skin
title_full	Microinjection of pruritogens in NGF-sensitized human skin
title_fullStr	Microinjection of pruritogens in NGF-sensitized human skin
title_full_unstemmed	Microinjection of pruritogens in NGF-sensitized human skin
title_sort	microinjection of pruritogens in ngf-sensitized human skin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8d4c5dba13f946068dab5b501ebb67c2
work_keys_str_mv	AT hansjurgensolinski microinjectionofpruritogensinngfsensitizedhumanskin AT romanrukwied microinjectionofpruritogensinngfsensitizedhumanskin AT martinschmelz microinjectionofpruritogensinngfsensitizedhumanskin
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Microinjection of pruritogens in NGF-sensitized human skin

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