Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.

In adults, the growth of blood vessels, a process known as angiogenesis, is essential for organ growth and repair. In many disorders including cancer, angiogenesis becomes excessive. The cellular origin of new vascular endothelial cells (ECs) during blood vessel growth in angiogenic situations has r...

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Autores principales:	Shentong Fang, Jing Wei, Nalle Pentinmikko, Hannele Leinonen, Petri Salven
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8d51ffca982842e9aba912fa4bc7db38
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spelling	oai:doaj.org-article:8d51ffca982842e9aba912fa4bc7db382021-11-18T05:37:24ZGeneration of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.1544-91731545-788510.1371/journal.pbio.1001407https://doaj.org/article/8d51ffca982842e9aba912fa4bc7db382012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23091420/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885In adults, the growth of blood vessels, a process known as angiogenesis, is essential for organ growth and repair. In many disorders including cancer, angiogenesis becomes excessive. The cellular origin of new vascular endothelial cells (ECs) during blood vessel growth in angiogenic situations has remained unknown. Here, we provide evidence for adult vascular endothelial stem cells (VESCs) that reside in the blood vessel wall endothelium. VESCs constitute a small subpopulation within CD117+ (c-kit+) ECs capable of undergoing clonal expansion while other ECs have a very limited proliferative capacity. Isolated VESCs can produce tens of millions of endothelial daughter cells in vitro. A single transplanted c-kit-expressing VESC by the phenotype lin-CD31+CD105+Sca1+CD117+ can generate in vivo functional blood vessels that connect to host circulation. VESCs also have long-term self-renewal capacity, a defining functional property of adult stem cells. To provide functional verification on the role of c-kit in VESCs, we show that a genetic deficit in endothelial c-kit expression markedly decreases total colony-forming VESCs. In vivo, c-kit expression deficit resulted in impaired EC proliferation and angiogenesis and retardation of tumor growth. Isolated VESCs could be used in cell-based therapies for cardiovascular repair to restore tissue vascularization after ischemic events. VESCs also provide a novel cellular target to block pathological angiogenesis and cancer growth.Shentong FangJing WeiNalle PentinmikkoHannele LeinonenPetri SalvenPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 10, Iss 10, p e1001407 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Shentong Fang Jing Wei Nalle Pentinmikko Hannele Leinonen Petri Salven Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
description	In adults, the growth of blood vessels, a process known as angiogenesis, is essential for organ growth and repair. In many disorders including cancer, angiogenesis becomes excessive. The cellular origin of new vascular endothelial cells (ECs) during blood vessel growth in angiogenic situations has remained unknown. Here, we provide evidence for adult vascular endothelial stem cells (VESCs) that reside in the blood vessel wall endothelium. VESCs constitute a small subpopulation within CD117+ (c-kit+) ECs capable of undergoing clonal expansion while other ECs have a very limited proliferative capacity. Isolated VESCs can produce tens of millions of endothelial daughter cells in vitro. A single transplanted c-kit-expressing VESC by the phenotype lin-CD31+CD105+Sca1+CD117+ can generate in vivo functional blood vessels that connect to host circulation. VESCs also have long-term self-renewal capacity, a defining functional property of adult stem cells. To provide functional verification on the role of c-kit in VESCs, we show that a genetic deficit in endothelial c-kit expression markedly decreases total colony-forming VESCs. In vivo, c-kit expression deficit resulted in impaired EC proliferation and angiogenesis and retardation of tumor growth. Isolated VESCs could be used in cell-based therapies for cardiovascular repair to restore tissue vascularization after ischemic events. VESCs also provide a novel cellular target to block pathological angiogenesis and cancer growth.
format	article
author	Shentong Fang Jing Wei Nalle Pentinmikko Hannele Leinonen Petri Salven
author_facet	Shentong Fang Jing Wei Nalle Pentinmikko Hannele Leinonen Petri Salven
author_sort	Shentong Fang
title	Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
title_short	Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
title_full	Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
title_fullStr	Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
title_full_unstemmed	Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
title_sort	generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8d51ffca982842e9aba912fa4bc7db38
work_keys_str_mv	AT shentongfang generationoffunctionalbloodvesselsfromasingleckitadultvascularendothelialstemcell AT jingwei generationoffunctionalbloodvesselsfromasingleckitadultvascularendothelialstemcell AT nallepentinmikko generationoffunctionalbloodvesselsfromasingleckitadultvascularendothelialstemcell AT hanneleleinonen generationoffunctionalbloodvesselsfromasingleckitadultvascularendothelialstemcell AT petrisalven generationoffunctionalbloodvesselsfromasingleckitadultvascularendothelialstemcell
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Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.

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