Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide

Introduction: With increasing antimicrobial resistance to common antibiotics, the development of alternative therapeutic strategies is necessary. Also, an antibacterial peptide with numerous behavior and different properties such as net charge, hydrophobicity, length, etc. could act against pathogen...

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Autores principales:	Kazem Ahmadi, Mahdi Fasihi Ramandi
Formato:	article
Lenguaje:	EN
Publicado:	Urology Research Center 2021
Materias:	antimicrobial peptide cationic peptide minimal inhibitory concentration minimal bactericidal concentration nitric oxide Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/8d5a5590834d479da4c073cdd3af6ae1
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spelling	oai:doaj.org-article:8d5a5590834d479da4c073cdd3af6ae12021-11-30T07:13:00ZEvaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide10.22034/TRU.2021.290437.10682717-042Xhttps://doaj.org/article/8d5a5590834d479da4c073cdd3af6ae12021-07-01T00:00:00Zhttps://www.transresurology.com/article_132939.htmlhttps://doaj.org/toc/2717-042XIntroduction: With increasing antimicrobial resistance to common antibiotics, the development of alternative therapeutic strategies is necessary. Also, an antibacterial peptide with numerous behavior and different properties such as net charge, hydrophobicity, length, etc. could act against pathogenic microorganisms. In recent years, novel peptides with activity against a wide range of bacteria have been introduced. Methods: In this study, seventeen pathogenic bacteria were chosen to study the antibacterial effect of K4 peptide using MIC and MBC assays. The therapeutic index (TI) of this peptide was experimentally calculated by the ratio of HC50 to MIC as a parameter to represent the specificity of AMP. In silico analysis was performed to predict the physicochemical properties, structures, and behavior of this peptide. In vitro cytotoxic effect of peptide was evaluated on the Hela cell line using MTT assay, and the amount of macrophage nitric oxide production was measured by the Griess method on the J774 cell line. Results: Peptide concentrations of 25-400 µg/ml were seen as the MIC value results for different bacteria. MBC assay showed such a result with a concentration of more than 25-400 µg/ml. The result of the hemolysis assay was 24 percent at a 1 mg/ml concentration. The amount of nitric oxide production of macrophage cell line J774 was 25.9873 µM at a 6.3 µg/ml peptide concentration. Conclusions: K4 peptide had a strong antibacterial effect on some bacteria such as B. melitensis. This peptide may have a role in immunity with nitric oxide production. Additionally, it enhances the bacterial killing mechanism of macrophages, making this peptide a potential agent against pathogens.Kazem AhmadiMahdi Fasihi Ramandi Urology Research Centerarticleantimicrobial peptidecationic peptideminimal inhibitory concentrationminimal bactericidal concentrationnitric oxideDiseases of the genitourinary system. UrologyRC870-923ENTranslational Research in Urology, Vol 3, Iss 2, Pp 59-66 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial peptide cationic peptide minimal inhibitory concentration minimal bactericidal concentration nitric oxide Diseases of the genitourinary system. Urology RC870-923
spellingShingle	antimicrobial peptide cationic peptide minimal inhibitory concentration minimal bactericidal concentration nitric oxide Diseases of the genitourinary system. Urology RC870-923 Kazem Ahmadi Mahdi Fasihi Ramandi Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide
description	Introduction: With increasing antimicrobial resistance to common antibiotics, the development of alternative therapeutic strategies is necessary. Also, an antibacterial peptide with numerous behavior and different properties such as net charge, hydrophobicity, length, etc. could act against pathogenic microorganisms. In recent years, novel peptides with activity against a wide range of bacteria have been introduced. Methods: In this study, seventeen pathogenic bacteria were chosen to study the antibacterial effect of K4 peptide using MIC and MBC assays. The therapeutic index (TI) of this peptide was experimentally calculated by the ratio of HC50 to MIC as a parameter to represent the specificity of AMP. In silico analysis was performed to predict the physicochemical properties, structures, and behavior of this peptide. In vitro cytotoxic effect of peptide was evaluated on the Hela cell line using MTT assay, and the amount of macrophage nitric oxide production was measured by the Griess method on the J774 cell line. Results: Peptide concentrations of 25-400 µg/ml were seen as the MIC value results for different bacteria. MBC assay showed such a result with a concentration of more than 25-400 µg/ml. The result of the hemolysis assay was 24 percent at a 1 mg/ml concentration. The amount of nitric oxide production of macrophage cell line J774 was 25.9873 µM at a 6.3 µg/ml peptide concentration. Conclusions: K4 peptide had a strong antibacterial effect on some bacteria such as B. melitensis. This peptide may have a role in immunity with nitric oxide production. Additionally, it enhances the bacterial killing mechanism of macrophages, making this peptide a potential agent against pathogens.
format	article
author	Kazem Ahmadi Mahdi Fasihi Ramandi
author_facet	Kazem Ahmadi Mahdi Fasihi Ramandi
author_sort	Kazem Ahmadi
title	Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide
title_short	Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide
title_full	Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide
title_fullStr	Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide
title_full_unstemmed	Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide
title_sort	evaluation of antibacterial and cytotoxic effects of k4 synthetic peptide
publisher	Urology Research Center
publishDate	2021
url	https://doaj.org/article/8d5a5590834d479da4c073cdd3af6ae1
work_keys_str_mv	AT kazemahmadi evaluationofantibacterialandcytotoxiceffectsofk4syntheticpeptide AT mahdifasihiramandi evaluationofantibacterialandcytotoxiceffectsofk4syntheticpeptide
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Evaluation of Antibacterial and Cytotoxic Effects of K4 Synthetic Peptide

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