Lack of inflammatory gene expression in bats: a unique role for a transcription repressor

Abstract In recent years viruses similar to those that appear to cause no overt disease in bats have spilled-over to humans and other species causing serious disease. Since pathology in such diseases is often attributed to an over-active inflammatory response, we tested the hypothesis that bat cells...

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Autores principales:	Arinjay Banerjee, Noreen Rapin, Trent Bollinger, Vikram Misra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d5d0ee9298e4d4ba52463be622bee19
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spelling	oai:doaj.org-article:8d5d0ee9298e4d4ba52463be622bee192021-12-02T12:30:53ZLack of inflammatory gene expression in bats: a unique role for a transcription repressor10.1038/s41598-017-01513-w2045-2322https://doaj.org/article/8d5d0ee9298e4d4ba52463be622bee192017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01513-whttps://doaj.org/toc/2045-2322Abstract In recent years viruses similar to those that appear to cause no overt disease in bats have spilled-over to humans and other species causing serious disease. Since pathology in such diseases is often attributed to an over-active inflammatory response, we tested the hypothesis that bat cells respond to stimulation of their receptors for viral ligands with a strong antiviral response, but unlike in human cells, the inflammatory response is not overtly activated. We compared the response of human and bat cells to poly(I:C), a viral double-stranded RNA surrogate. We measured transcripts for several inflammatory, interferon and interferon stimulated genes using quantitative real-time PCR and observed that human and bat cells both, when stimulated with poly(I:C), contained higher levels of transcripts for interferon beta than unstimulated cells. In contrast, only human cells expressed robust amount of RNA for TNFα, a cell signaling protein involved in systemic inflammation. We examined the bat TNFα promoter and found a potential repressor (c-Rel) binding motif. We demonstrated that c-Rel binds to the putative c-Rel motif in the promoter and knocking down c-Rel transcripts significantly increased basal levels of TNFα transcripts. Our results suggest bats may have a unique mechanism to suppress inflammatory pathology.Arinjay BanerjeeNoreen RapinTrent BollingerVikram MisraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arinjay Banerjee Noreen Rapin Trent Bollinger Vikram Misra Lack of inflammatory gene expression in bats: a unique role for a transcription repressor
description	Abstract In recent years viruses similar to those that appear to cause no overt disease in bats have spilled-over to humans and other species causing serious disease. Since pathology in such diseases is often attributed to an over-active inflammatory response, we tested the hypothesis that bat cells respond to stimulation of their receptors for viral ligands with a strong antiviral response, but unlike in human cells, the inflammatory response is not overtly activated. We compared the response of human and bat cells to poly(I:C), a viral double-stranded RNA surrogate. We measured transcripts for several inflammatory, interferon and interferon stimulated genes using quantitative real-time PCR and observed that human and bat cells both, when stimulated with poly(I:C), contained higher levels of transcripts for interferon beta than unstimulated cells. In contrast, only human cells expressed robust amount of RNA for TNFα, a cell signaling protein involved in systemic inflammation. We examined the bat TNFα promoter and found a potential repressor (c-Rel) binding motif. We demonstrated that c-Rel binds to the putative c-Rel motif in the promoter and knocking down c-Rel transcripts significantly increased basal levels of TNFα transcripts. Our results suggest bats may have a unique mechanism to suppress inflammatory pathology.
format	article
author	Arinjay Banerjee Noreen Rapin Trent Bollinger Vikram Misra
author_facet	Arinjay Banerjee Noreen Rapin Trent Bollinger Vikram Misra
author_sort	Arinjay Banerjee
title	Lack of inflammatory gene expression in bats: a unique role for a transcription repressor
title_short	Lack of inflammatory gene expression in bats: a unique role for a transcription repressor
title_full	Lack of inflammatory gene expression in bats: a unique role for a transcription repressor
title_fullStr	Lack of inflammatory gene expression in bats: a unique role for a transcription repressor
title_full_unstemmed	Lack of inflammatory gene expression in bats: a unique role for a transcription repressor
title_sort	lack of inflammatory gene expression in bats: a unique role for a transcription repressor
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8d5d0ee9298e4d4ba52463be622bee19
work_keys_str_mv	AT arinjaybanerjee lackofinflammatorygeneexpressioninbatsauniqueroleforatranscriptionrepressor AT noreenrapin lackofinflammatorygeneexpressioninbatsauniqueroleforatranscriptionrepressor AT trentbollinger lackofinflammatorygeneexpressioninbatsauniqueroleforatranscriptionrepressor AT vikrammisra lackofinflammatorygeneexpressioninbatsauniqueroleforatranscriptionrepressor
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Lack of inflammatory gene expression in bats: a unique role for a transcription repressor

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