Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.

Malaria, a disease caused by Plasmodium parasites, remains a major threat to public health globally. It is the most common disease in patients with sleeping sickness, another parasitic illness, caused by Trypanosoma brucei. We have previously shown that a T. brucei infection impairs a secondary P. b...

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Autores principales:	Adriana Temporão, Margarida Sanches-Vaz, Rafael Luís, Helena Nunes-Cabaço, Terry K Smith, Miguel Prudêncio, Luisa M Figueiredo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/8d690560e2284aef846646287f57d515
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spelling	oai:doaj.org-article:8d690560e2284aef846646287f57d5152021-12-02T20:23:33ZExcreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.1935-27271935-273510.1371/journal.pntd.0009912https://doaj.org/article/8d690560e2284aef846646287f57d5152021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009912https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Malaria, a disease caused by Plasmodium parasites, remains a major threat to public health globally. It is the most common disease in patients with sleeping sickness, another parasitic illness, caused by Trypanosoma brucei. We have previously shown that a T. brucei infection impairs a secondary P. berghei liver infection and decreases malaria severity in mice. However, whether this effect requires an active trypanosome infection remained unknown. Here, we show that Plasmodium liver infection can also be inhibited by the serum of a mouse previously infected by T. brucei and by total protein lysates of this kinetoplastid. Biochemical characterisation showed that the anti-Plasmodium activity of the total T. brucei lysates depends on its protein fraction, but is independent of the abundant variant surface glycoprotein. Finally, we found that the protein(s) responsible for the inhibition of Plasmodium infection is/are present within a fraction of ~350 proteins that are excreted to the bloodstream of the host. We conclude that the defence mechanism developed by trypanosomes against Plasmodium relies on protein excretion. This study opens the door to the identification of novel antiplasmodial intervention strategies.Adriana TemporãoMargarida Sanches-VazRafael LuísHelena Nunes-CabaçoTerry K SmithMiguel PrudêncioLuisa M FigueiredoPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e0009912 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Adriana Temporão Margarida Sanches-Vaz Rafael Luís Helena Nunes-Cabaço Terry K Smith Miguel Prudêncio Luisa M Figueiredo Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.
description	Malaria, a disease caused by Plasmodium parasites, remains a major threat to public health globally. It is the most common disease in patients with sleeping sickness, another parasitic illness, caused by Trypanosoma brucei. We have previously shown that a T. brucei infection impairs a secondary P. berghei liver infection and decreases malaria severity in mice. However, whether this effect requires an active trypanosome infection remained unknown. Here, we show that Plasmodium liver infection can also be inhibited by the serum of a mouse previously infected by T. brucei and by total protein lysates of this kinetoplastid. Biochemical characterisation showed that the anti-Plasmodium activity of the total T. brucei lysates depends on its protein fraction, but is independent of the abundant variant surface glycoprotein. Finally, we found that the protein(s) responsible for the inhibition of Plasmodium infection is/are present within a fraction of ~350 proteins that are excreted to the bloodstream of the host. We conclude that the defence mechanism developed by trypanosomes against Plasmodium relies on protein excretion. This study opens the door to the identification of novel antiplasmodial intervention strategies.
format	article
author	Adriana Temporão Margarida Sanches-Vaz Rafael Luís Helena Nunes-Cabaço Terry K Smith Miguel Prudêncio Luisa M Figueiredo
author_facet	Adriana Temporão Margarida Sanches-Vaz Rafael Luís Helena Nunes-Cabaço Terry K Smith Miguel Prudêncio Luisa M Figueiredo
author_sort	Adriana Temporão
title	Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.
title_short	Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.
title_full	Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.
title_fullStr	Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.
title_full_unstemmed	Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.
title_sort	excreted trypanosoma brucei proteins inhibit plasmodium hepatic infection.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8d690560e2284aef846646287f57d515
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Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.

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