Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials

Lionel F Gamarra1,2, Antonio J daCosta-Filho3, Javier B Mamani1, Rita de Cassia Ruiz4, Lorena F Pavon1, Tatiana T Sibov1, Ernanni D Vieira3, Andr&eacute; C Silva1, Walter M Pontuschka5, Edson Amaro Jr1,21Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, S&atilde;o Pau...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lionel F Gamarra, Antonio J daCosta-Filho, Javier B Mamani, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8d6d72aa44514f12ab136a9f18a21bfb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8d6d72aa44514f12ab136a9f18a21bfb
record_format	dspace
spelling	oai:doaj.org-article:8d6d72aa44514f12ab136a9f18a21bfb2021-12-02T00:37:47ZFerromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials1176-91141178-2013https://doaj.org/article/8d6d72aa44514f12ab136a9f18a21bfb2010-03-01T00:00:00Zhttp://www.dovepress.com/ferromagnetic-resonance-for-the-quantification-of-superparamagnetic-ir-a4129https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Lionel F Gamarra1,2, Antonio J daCosta-Filho3, Javier B Mamani1, Rita de Cassia Ruiz4, Lorena F Pavon1, Tatiana T Sibov1, Ernanni D Vieira3, Andr&eacute; C Silva1, Walter M Pontuschka5, Edson Amaro Jr1,21Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, S&atilde;o Paulo, Brazil; 2Instituto de Radiologia, Faculdade de Medicina, Universidade de S&atilde;o Paulo, S&atilde;o Paulo, Brazil; 3Instituto de F&iacute;sica de S&atilde;o Carlos, Universidade de S&atilde;o Paulo, S&atilde;o Carlos, Brazil; 4Instituto Butantan, S&atilde;o Paulo, Brazil; 5Instituto de F&iacute;sica, Universidade de S&atilde;o Paulo, S&atilde;o Paulo, BrazilAbstract: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).Keywords: quantification, FMR, ferrofluid, biodistribution, nanoparticles Lionel F GamarraAntonio J daCosta-FilhoJavier B Mamaniet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 203-211 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lionel F Gamarra Antonio J daCosta-Filho Javier B Mamani et al Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
description	Lionel F Gamarra1,2, Antonio J daCosta-Filho3, Javier B Mamani1, Rita de Cassia Ruiz4, Lorena F Pavon1, Tatiana T Sibov1, Ernanni D Vieira3, Andr&eacute; C Silva1, Walter M Pontuschka5, Edson Amaro Jr1,21Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, S&atilde;o Paulo, Brazil; 2Instituto de Radiologia, Faculdade de Medicina, Universidade de S&atilde;o Paulo, S&atilde;o Paulo, Brazil; 3Instituto de F&iacute;sica de S&atilde;o Carlos, Universidade de S&atilde;o Paulo, S&atilde;o Carlos, Brazil; 4Instituto Butantan, S&atilde;o Paulo, Brazil; 5Instituto de F&iacute;sica, Universidade de S&atilde;o Paulo, S&atilde;o Paulo, BrazilAbstract: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).Keywords: quantification, FMR, ferrofluid, biodistribution, nanoparticles
format	article
author	Lionel F Gamarra Antonio J daCosta-Filho Javier B Mamani et al
author_facet	Lionel F Gamarra Antonio J daCosta-Filho Javier B Mamani et al
author_sort	Lionel F Gamarra
title	Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
title_short	Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
title_full	Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
title_fullStr	Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
title_full_unstemmed	Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
title_sort	ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/8d6d72aa44514f12ab136a9f18a21bfb
work_keys_str_mv	AT lionelfgamarra ferromagneticresonanceforthequantificationofsuperparamagneticironoxidenanoparticlesinbiologicalmaterials AT antoniojdacostafilho ferromagneticresonanceforthequantificationofsuperparamagneticironoxidenanoparticlesinbiologicalmaterials AT javierbmamani ferromagneticresonanceforthequantificationofsuperparamagneticironoxidenanoparticlesinbiologicalmaterials AT etal ferromagneticresonanceforthequantificationofsuperparamagneticironoxidenanoparticlesinbiologicalmaterials
_version_	1718403568835231744

Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials

Ejemplares similares