Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs

Santosh Dhakal,1,2 Xingguo Cheng,3 John Salcido,3 Sankar Renu,1,2 Kathy Bondra,1,2 Yashavantha Shaan Lakshmanappa,1,2 Christina Misch,1,2 Shristi Ghimire,1,2 Ninoshkaly Feliciano-Ruiz,1,2 Bradley Hogshead,1,2 Steven Krakowka,4 Kenneth Carson,3 Joseph McDonough,3 Chang Won Lee,1,2 Gourapura J Renukar...

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Autores principales:	Dhakal S, Cheng X, Salcido J, Renu S, Bondra K, Lakshmanappa YS, Misch C, Ghimire S, Feliciano-Ruiz N, Hogshead B, Krakowka S, Carson K, McDonough J, Lee CW, Renukaradhya GJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Influenza A virus peptides Liposome nanoparticles Monosodium urate crystal adjuvant Intranasal vaccination Swine influenza virus Pigs Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8d6e82ecc95d43ea8eb8ce5298832869
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spelling	oai:doaj.org-article:8d6e82ecc95d43ea8eb8ce52988328692021-12-02T02:32:27ZLiposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs1178-2013https://doaj.org/article/8d6e82ecc95d43ea8eb8ce52988328692018-10-01T00:00:00Zhttps://www.dovepress.com/liposomal-nanoparticle-based-conserved-peptide-influenza-vaccine-and-m-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Santosh Dhakal,1,2 Xingguo Cheng,3 John Salcido,3 Sankar Renu,1,2 Kathy Bondra,1,2 Yashavantha Shaan Lakshmanappa,1,2 Christina Misch,1,2 Shristi Ghimire,1,2 Ninoshkaly Feliciano-Ruiz,1,2 Bradley Hogshead,1,2 Steven Krakowka,4 Kenneth Carson,3 Joseph McDonough,3 Chang Won Lee,1,2 Gourapura J Renukaradhya1,2 1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA; 2Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA; 3Pharmaceuticals and Bioengineering Department, Chemistry and Chemical Engineering Division, Southwest Research Institute, San Antonio, TX 78238-0510, USA; 4The Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA Background: Influenza (flu) is a constant threat to humans and animals, and vaccination is one of the most effective ways to mitigate the disease. Due to incomplete protection induced by current flu vaccines, development of novel flu vaccine candidates is warranted to achieve greater efficacy against constantly evolving flu viruses. Methods: In the present study, we used liposome nanoparticle (<200 nm diameter)-based subunit flu vaccine containing ten encapsulated highly conserved B and T cell epitope peptides to induce protective immune response against a zoonotic swine influenza A virus (SwIAV) H1N1 challenge infection in a pig model. Furthermore, we used monosodium urate (MSU) crystals as an adjuvant and co-administered the vaccine formulation as an intranasal mist to flu-free nursery pigs, twice at 3-week intervals. Results: Liposome peptides flu vaccine delivered with MSU adjuvant improved the hemagglutination inhibition antibody titer and mucosal IgA response against the SwIAV challenge and also against two other highly genetically variant IAVs. Liposomal vaccines also enhanced the frequency of peptides and virus-specific T-helper/memory cells and IFN-γ response. The improved specific cellular and mucosal humoral immune responses in adjuvanted liposomal peptides flu vaccine partially protected pigs from flu-induced fever and pneumonic lesions, and reduced the nasal virus shedding and viral load in the lungs. Conclusion: Overall, our study shows great promise for using liposome and MSU adjuvant-based subunit flu vaccine through the intranasal route, and provides scope for future, pre-clinical investigations in a pig model for developing potent human intranasal subunit flu vaccines. Keywords: influenza A virus peptides, liposome nanoparticles, monosodium urate crystal adjuvant, intranasal vaccination, swine influenza virus, pigsDhakal SCheng XSalcido JRenu SBondra KLakshmanappa YSMisch CGhimire SFeliciano-Ruiz NHogshead BKrakowka SCarson KMcDonough JLee CWRenukaradhya GJDove Medical PressarticleInfluenza A virus peptidesLiposome nanoparticlesMonosodium urate crystal adjuvantIntranasal vaccinationSwine influenza virusPigsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6699-6715 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Influenza A virus peptides Liposome nanoparticles Monosodium urate crystal adjuvant Intranasal vaccination Swine influenza virus Pigs Medicine (General) R5-920
spellingShingle	Influenza A virus peptides Liposome nanoparticles Monosodium urate crystal adjuvant Intranasal vaccination Swine influenza virus Pigs Medicine (General) R5-920 Dhakal S Cheng X Salcido J Renu S Bondra K Lakshmanappa YS Misch C Ghimire S Feliciano-Ruiz N Hogshead B Krakowka S Carson K McDonough J Lee CW Renukaradhya GJ Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs
description	Santosh Dhakal,1,2 Xingguo Cheng,3 John Salcido,3 Sankar Renu,1,2 Kathy Bondra,1,2 Yashavantha Shaan Lakshmanappa,1,2 Christina Misch,1,2 Shristi Ghimire,1,2 Ninoshkaly Feliciano-Ruiz,1,2 Bradley Hogshead,1,2 Steven Krakowka,4 Kenneth Carson,3 Joseph McDonough,3 Chang Won Lee,1,2 Gourapura J Renukaradhya1,2 1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA; 2Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA; 3Pharmaceuticals and Bioengineering Department, Chemistry and Chemical Engineering Division, Southwest Research Institute, San Antonio, TX 78238-0510, USA; 4The Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA Background: Influenza (flu) is a constant threat to humans and animals, and vaccination is one of the most effective ways to mitigate the disease. Due to incomplete protection induced by current flu vaccines, development of novel flu vaccine candidates is warranted to achieve greater efficacy against constantly evolving flu viruses. Methods: In the present study, we used liposome nanoparticle (<200 nm diameter)-based subunit flu vaccine containing ten encapsulated highly conserved B and T cell epitope peptides to induce protective immune response against a zoonotic swine influenza A virus (SwIAV) H1N1 challenge infection in a pig model. Furthermore, we used monosodium urate (MSU) crystals as an adjuvant and co-administered the vaccine formulation as an intranasal mist to flu-free nursery pigs, twice at 3-week intervals. Results: Liposome peptides flu vaccine delivered with MSU adjuvant improved the hemagglutination inhibition antibody titer and mucosal IgA response against the SwIAV challenge and also against two other highly genetically variant IAVs. Liposomal vaccines also enhanced the frequency of peptides and virus-specific T-helper/memory cells and IFN-γ response. The improved specific cellular and mucosal humoral immune responses in adjuvanted liposomal peptides flu vaccine partially protected pigs from flu-induced fever and pneumonic lesions, and reduced the nasal virus shedding and viral load in the lungs. Conclusion: Overall, our study shows great promise for using liposome and MSU adjuvant-based subunit flu vaccine through the intranasal route, and provides scope for future, pre-clinical investigations in a pig model for developing potent human intranasal subunit flu vaccines. Keywords: influenza A virus peptides, liposome nanoparticles, monosodium urate crystal adjuvant, intranasal vaccination, swine influenza virus, pigs
format	article
author	Dhakal S Cheng X Salcido J Renu S Bondra K Lakshmanappa YS Misch C Ghimire S Feliciano-Ruiz N Hogshead B Krakowka S Carson K McDonough J Lee CW Renukaradhya GJ
author_facet	Dhakal S Cheng X Salcido J Renu S Bondra K Lakshmanappa YS Misch C Ghimire S Feliciano-Ruiz N Hogshead B Krakowka S Carson K McDonough J Lee CW Renukaradhya GJ
author_sort	Dhakal S
title	Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs
title_short	Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs
title_full	Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs
title_fullStr	Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs
title_full_unstemmed	Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs
title_sort	liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/8d6e82ecc95d43ea8eb8ce5298832869
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Liposomal nanoparticle-based conserved peptide influenza vaccine and monosodium urate crystal adjuvant elicit protective immune response in pigs

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