Morin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks
ABSTRACT: Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and parasitic aspergillus, mainly existing in cereals, peanuts, corn, and other crops, which seriously endanger poultry, human health, and environment. Morin, a flavonoid compound extracted from moraceae plants, p...
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Elsevier
2021
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oai:doaj.org-article:8d784670b9bd4eac8fc2e2bb89dc18dd2021-11-24T04:22:53ZMorin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks0032-579110.1016/j.psj.2021.101513https://doaj.org/article/8d784670b9bd4eac8fc2e2bb89dc18dd2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005356https://doaj.org/toc/0032-5791ABSTRACT: Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and parasitic aspergillus, mainly existing in cereals, peanuts, corn, and other crops, which seriously endanger poultry, human health, and environment. Morin, a flavonoid compound extracted from moraceae plants, possess antioxidant, antibacterial, and anti-inflammatory effects. However, whether morin has a protective effect on AFB1-induced liver and kidney damage in chicks has not been specifically reported. In this study, we mainly confirmed the protective effect of morin on AFB1-induced liver and kidney damage in chicks and clarified its mechanism. It was found that morin can significantly reduce the liver biochemical indicators of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and kidney indicators of creatinine (CRE) and urea nitrogen (BUN) levels. Meanwhile, histopathological examination showed that morin effectively relieved AFB1-caused liver damage, including hepatocyte disruption, swelling, and inflammatory cell infiltration, and effectively relieved kidney damage, including renal cell necrosis, exfoliation, and vacuolization. Further investigation of its mechanism demonstrated that morin significantly inhibited AFB1-induced heterophil extracellular traps (HETs) release, and decreased the level of malondialdehyde (MDA) but increased the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in vivo. Moreover, quantitative real-time PCR (qRT-PCR) analysis showed that morin also significantly decreased AFB1-induced mRNA expressions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), caspase-1, caspase-3, and caspase-11. In conclusion, all results confirmed that morin could protect AFB1-caused liver and kidney damage by inhibiting HETs release, regulating oxidative stress, and inhibiting inflammatory response, suggesting that morin can be utilized as a potential drug for prevention and treatment of aflatoxicosis in poultry breeding industry.Xinxin GaoJingnan XuLiqiang JiangWei LiuHongrong HongYuxiao QianShurou LiWenlong HuangHaiguang ZhaoZhengtao YangQuan LiuZhengkai WeiElsevierarticlemorinaflatoxin B1heterophil extracellular trapsinflammatory responseoxidative stressAnimal cultureSF1-1100ENPoultry Science, Vol 100, Iss 12, Pp 101513- (2021) |
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| topic |
morin aflatoxin B1 heterophil extracellular traps inflammatory response oxidative stress Animal culture SF1-1100 |
| spellingShingle |
morin aflatoxin B1 heterophil extracellular traps inflammatory response oxidative stress Animal culture SF1-1100 Xinxin Gao Jingnan Xu Liqiang Jiang Wei Liu Hongrong Hong Yuxiao Qian Shurou Li Wenlong Huang Haiguang Zhao Zhengtao Yang Quan Liu Zhengkai Wei Morin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks |
| description |
ABSTRACT: Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and parasitic aspergillus, mainly existing in cereals, peanuts, corn, and other crops, which seriously endanger poultry, human health, and environment. Morin, a flavonoid compound extracted from moraceae plants, possess antioxidant, antibacterial, and anti-inflammatory effects. However, whether morin has a protective effect on AFB1-induced liver and kidney damage in chicks has not been specifically reported. In this study, we mainly confirmed the protective effect of morin on AFB1-induced liver and kidney damage in chicks and clarified its mechanism. It was found that morin can significantly reduce the liver biochemical indicators of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and kidney indicators of creatinine (CRE) and urea nitrogen (BUN) levels. Meanwhile, histopathological examination showed that morin effectively relieved AFB1-caused liver damage, including hepatocyte disruption, swelling, and inflammatory cell infiltration, and effectively relieved kidney damage, including renal cell necrosis, exfoliation, and vacuolization. Further investigation of its mechanism demonstrated that morin significantly inhibited AFB1-induced heterophil extracellular traps (HETs) release, and decreased the level of malondialdehyde (MDA) but increased the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in vivo. Moreover, quantitative real-time PCR (qRT-PCR) analysis showed that morin also significantly decreased AFB1-induced mRNA expressions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), caspase-1, caspase-3, and caspase-11. In conclusion, all results confirmed that morin could protect AFB1-caused liver and kidney damage by inhibiting HETs release, regulating oxidative stress, and inhibiting inflammatory response, suggesting that morin can be utilized as a potential drug for prevention and treatment of aflatoxicosis in poultry breeding industry. |
| format |
article |
| author |
Xinxin Gao Jingnan Xu Liqiang Jiang Wei Liu Hongrong Hong Yuxiao Qian Shurou Li Wenlong Huang Haiguang Zhao Zhengtao Yang Quan Liu Zhengkai Wei |
| author_facet |
Xinxin Gao Jingnan Xu Liqiang Jiang Wei Liu Hongrong Hong Yuxiao Qian Shurou Li Wenlong Huang Haiguang Zhao Zhengtao Yang Quan Liu Zhengkai Wei |
| author_sort |
Xinxin Gao |
| title |
Morin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks |
| title_short |
Morin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks |
| title_full |
Morin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks |
| title_fullStr |
Morin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks |
| title_full_unstemmed |
Morin alleviates aflatoxin B1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks |
| title_sort |
morin alleviates aflatoxin b1-induced liver and kidney injury by inhibiting heterophil extracellular traps release, oxidative stress and inflammatory responses in chicks |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/8d784670b9bd4eac8fc2e2bb89dc18dd |
| work_keys_str_mv |
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| _version_ |
1718416047138144256 |