Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

The popular genetically-encoded Ca2+ indicator, GCaMP, has several side-effects. Here the authors show that GCaMP containing CaM interferes with gating and signaling of L-type calcium channels, which disrupts Ca2+ dynamics and gene expression, and develop GCaMP-X to overcome these limitations.

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Autores principales:	Yaxiong Yang, Nan Liu, Yuanyuan He, Yuxia Liu, Lin Ge, Linzhi Zou, Sen Song, Wei Xiong, Xiaodong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8d7a563ae4be4927b40a8d6a24ea980d
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spelling	oai:doaj.org-article:8d7a563ae4be4927b40a8d6a24ea980d2021-12-02T17:32:48ZImproved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP10.1038/s41467-018-03719-62041-1723https://doaj.org/article/8d7a563ae4be4927b40a8d6a24ea980d2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03719-6https://doaj.org/toc/2041-1723The popular genetically-encoded Ca2+ indicator, GCaMP, has several side-effects. Here the authors show that GCaMP containing CaM interferes with gating and signaling of L-type calcium channels, which disrupts Ca2+ dynamics and gene expression, and develop GCaMP-X to overcome these limitations.Yaxiong YangNan LiuYuanyuan HeYuxia LiuLin GeLinzhi ZouSen SongWei XiongXiaodong LiuNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-18 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yaxiong Yang Nan Liu Yuanyuan He Yuxia Liu Lin Ge Linzhi Zou Sen Song Wei Xiong Xiaodong Liu Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
description	The popular genetically-encoded Ca2+ indicator, GCaMP, has several side-effects. Here the authors show that GCaMP containing CaM interferes with gating and signaling of L-type calcium channels, which disrupts Ca2+ dynamics and gene expression, and develop GCaMP-X to overcome these limitations.
format	article
author	Yaxiong Yang Nan Liu Yuanyuan He Yuxia Liu Lin Ge Linzhi Zou Sen Song Wei Xiong Xiaodong Liu
author_facet	Yaxiong Yang Nan Liu Yuanyuan He Yuxia Liu Lin Ge Linzhi Zou Sen Song Wei Xiong Xiaodong Liu
author_sort	Yaxiong Yang
title	Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
title_short	Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
title_full	Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
title_fullStr	Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
title_full_unstemmed	Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
title_sort	improved calcium sensor gcamp-x overcomes the calcium channel perturbations induced by the calmodulin in gcamp
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8d7a563ae4be4927b40a8d6a24ea980d
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Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

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