Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots

Metalloid contamination, such as arsenic poisoning, poses a significant environmental problem, reducing plant productivity and putting human health at risk. Phytohormones are known to regulate arsenic stress; however, the function of strigolactones (SLs) in arsenic stress tolerance in rice is rarely...

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Autores principales:	Mohammad Golam Mostofa, Chien Van Ha, Md. Mezanur Rahman, Kien Huu Nguyen, Sanjida Sultana Keya, Yasuko Watanabe, Misao Itouga, Abeer Hashem, Elsayed Fathi Abd_Allah, Masayuki Fujita, Lam-Son Phan Tran
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	arsenate stress <i>dwarf</i> mutants enzyme activation glutathione oxidative damage rice Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8d7b2b8f0a3641fa8279f4a8abc6e65e
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spelling	oai:doaj.org-article:8d7b2b8f0a3641fa8279f4a8abc6e65e2021-11-25T16:28:58ZStrigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots10.3390/antiox101118152076-3921https://doaj.org/article/8d7b2b8f0a3641fa8279f4a8abc6e65e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1815https://doaj.org/toc/2076-3921Metalloid contamination, such as arsenic poisoning, poses a significant environmental problem, reducing plant productivity and putting human health at risk. Phytohormones are known to regulate arsenic stress; however, the function of strigolactones (SLs) in arsenic stress tolerance in rice is rarely investigated. Here, we investigated shoot responses of wild-type (WT) and SL-deficient <i>d10</i> and <i>d17 </i>rice mutants under arsenate stress to elucidate SLs’ roles in rice adaptation to arsenic. Under arsenate stress, the <i>d10</i> and <i>d17</i> mutants displayed severe growth abnormalities, including phenotypic aberrations, chlorosis and biomass loss, relative to WT. Arsenate stress activated the SL-biosynthetic pathway by enhancing the expression of SL-biosynthetic genes <i>D10</i> and <i>D17</i> in WT shoots. No differences in arsenic levels between WT and SL-biosynthetic mutants were found from Inductively Coupled Plasma-Mass Spectrometry analysis, demonstrating that the greater growth defects of mutant plants did not result from accumulated arsenic in shoots. The <i>d10</i> and <i>d17</i> plants had higher levels of reactive oxygen species, water loss, electrolyte leakage and membrane damage but lower activities of superoxide dismutase, ascorbate peroxidase, glutathione peroxidase and glutathione <i>S</i>-transferase than did the WT, implying that arsenate caused substantial oxidative stress in the SL mutants. Furthermore, WT plants had higher glutathione (GSH) contents and transcript levels of <i>OsGSH1</i>,<i> OsGSH2</i>,<i> OsPCS1</i> and <i>OsABCC1 </i>in their shoots, indicating an upregulation of GSH-assisted arsenic sequestration into vacuoles. We conclude that arsenate stress activated SL biosynthesis, which led to enhanced arsenate tolerance through the stimulation of cellular antioxidant defense systems and vacuolar sequestration of arsenic, suggesting a novel role for SLs in rice adaptation to arsenic stress. Our findings have significant implications in the development of arsenic-resistant rice varieties for safe and sustainable rice production in arsenic-polluted soils.Mohammad Golam MostofaChien Van HaMd. Mezanur RahmanKien Huu NguyenSanjida Sultana KeyaYasuko WatanabeMisao ItougaAbeer HashemElsayed Fathi Abd_AllahMasayuki FujitaLam-Son Phan TranMDPI AGarticlearsenate stress<i>dwarf</i> mutantsenzyme activationglutathioneoxidative damagericeTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1815, p 1815 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	arsenate stress <i>dwarf</i> mutants enzyme activation glutathione oxidative damage rice Therapeutics. Pharmacology RM1-950
spellingShingle	arsenate stress <i>dwarf</i> mutants enzyme activation glutathione oxidative damage rice Therapeutics. Pharmacology RM1-950 Mohammad Golam Mostofa Chien Van Ha Md. Mezanur Rahman Kien Huu Nguyen Sanjida Sultana Keya Yasuko Watanabe Misao Itouga Abeer Hashem Elsayed Fathi Abd_Allah Masayuki Fujita Lam-Son Phan Tran Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
description	Metalloid contamination, such as arsenic poisoning, poses a significant environmental problem, reducing plant productivity and putting human health at risk. Phytohormones are known to regulate arsenic stress; however, the function of strigolactones (SLs) in arsenic stress tolerance in rice is rarely investigated. Here, we investigated shoot responses of wild-type (WT) and SL-deficient <i>d10</i> and <i>d17 </i>rice mutants under arsenate stress to elucidate SLs’ roles in rice adaptation to arsenic. Under arsenate stress, the <i>d10</i> and <i>d17</i> mutants displayed severe growth abnormalities, including phenotypic aberrations, chlorosis and biomass loss, relative to WT. Arsenate stress activated the SL-biosynthetic pathway by enhancing the expression of SL-biosynthetic genes <i>D10</i> and <i>D17</i> in WT shoots. No differences in arsenic levels between WT and SL-biosynthetic mutants were found from Inductively Coupled Plasma-Mass Spectrometry analysis, demonstrating that the greater growth defects of mutant plants did not result from accumulated arsenic in shoots. The <i>d10</i> and <i>d17</i> plants had higher levels of reactive oxygen species, water loss, electrolyte leakage and membrane damage but lower activities of superoxide dismutase, ascorbate peroxidase, glutathione peroxidase and glutathione <i>S</i>-transferase than did the WT, implying that arsenate caused substantial oxidative stress in the SL mutants. Furthermore, WT plants had higher glutathione (GSH) contents and transcript levels of <i>OsGSH1</i>,<i> OsGSH2</i>,<i> OsPCS1</i> and <i>OsABCC1 </i>in their shoots, indicating an upregulation of GSH-assisted arsenic sequestration into vacuoles. We conclude that arsenate stress activated SL biosynthesis, which led to enhanced arsenate tolerance through the stimulation of cellular antioxidant defense systems and vacuolar sequestration of arsenic, suggesting a novel role for SLs in rice adaptation to arsenic stress. Our findings have significant implications in the development of arsenic-resistant rice varieties for safe and sustainable rice production in arsenic-polluted soils.
format	article
author	Mohammad Golam Mostofa Chien Van Ha Md. Mezanur Rahman Kien Huu Nguyen Sanjida Sultana Keya Yasuko Watanabe Misao Itouga Abeer Hashem Elsayed Fathi Abd_Allah Masayuki Fujita Lam-Son Phan Tran
author_facet	Mohammad Golam Mostofa Chien Van Ha Md. Mezanur Rahman Kien Huu Nguyen Sanjida Sultana Keya Yasuko Watanabe Misao Itouga Abeer Hashem Elsayed Fathi Abd_Allah Masayuki Fujita Lam-Son Phan Tran
author_sort	Mohammad Golam Mostofa
title	Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_short	Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_full	Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_fullStr	Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_full_unstemmed	Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_sort	strigolactones modulate cellular antioxidant defense mechanisms to mitigate arsenate toxicity in rice shoots
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8d7b2b8f0a3641fa8279f4a8abc6e65e
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Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots

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