Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars

(1) Background: Although resistance to pathogens and pests has been researched in many potato cultivars and breeding lines with DNA markers, there is scarce evidence as to the efficiency of the marker-assisted selection (MAS) for these traits when applied at the early stages of breeding. A goal of t...

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Autores principales:	Mariya P. Beketova, Nadezhda A. Chalaya, Nadezhda M. Zoteyeva, Alena A. Gurina, Mariya A. Kuznetsova, Miles Armstrong, Ingo Hein, Polina E. Drobyazina, Emil E. Khavkin, Elena V. Rogozina
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Rpi</i> genes parental lines hybrid progeny dRenSeq SCAR markers Agriculture S
Acceso en línea:	https://doaj.org/article/8d87a950df2d4f98848189c6209eb2a0
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spelling	oai:doaj.org-article:8d87a950df2d4f98848189c6209eb2a02021-11-25T16:06:03ZCombination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars10.3390/agronomy111121922073-4395https://doaj.org/article/8d87a950df2d4f98848189c6209eb2a02021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2192https://doaj.org/toc/2073-4395(1) Background: Although resistance to pathogens and pests has been researched in many potato cultivars and breeding lines with DNA markers, there is scarce evidence as to the efficiency of the marker-assisted selection (MAS) for these traits when applied at the early stages of breeding. A goal of this study was to estimate the potential of affordable DNA markers to track resistance genes that are effective against the pathogen <i>Phytophthora infestans</i> (<i>Rpi</i> genes)<i>,</i> as a practical breeding tool on a progeny of 68 clones derived from a cross between the cultivar Sudarynya and the hybrid 13/11-09. (2) Methods: this population was studied for four years to elucidate the distribution of late blight (LB) resistance and other agronomical desirable or simple to phenotype traits such as tuber and flower pigmentation, yield capacity and structure. LB resistance was phenotypically evaluated following natural and artificial infection and the presence/absence of nine <i>Rpi</i> genes was assessed with 11 sequence-characterized amplified region (SCAR) markers. To validate this analysis, the profile of <i>Rpi</i> genes in the 13/11-09 parent was established using diagnostic resistance gene enrichment sequencing (dRenSeq) as a gold standard. (3) Results: at the early stages of a breeding program, when screening the segregation of F1 offspring, MAS can halve the workload and selected SCAR markers for <i>Rpi</i> genes provide useful tools.Mariya P. BeketovaNadezhda A. ChalayaNadezhda M. ZoteyevaAlena A. GurinaMariya A. KuznetsovaMiles ArmstrongIngo HeinPolina E. DrobyazinaEmil E. KhavkinElena V. RogozinaMDPI AGarticle<i>Rpi</i> genesparental lineshybrid progenydRenSeqSCAR markersAgricultureSENAgronomy, Vol 11, Iss 2192, p 2192 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Rpi</i> genes parental lines hybrid progeny dRenSeq SCAR markers Agriculture S
spellingShingle	<i>Rpi</i> genes parental lines hybrid progeny dRenSeq SCAR markers Agriculture S Mariya P. Beketova Nadezhda A. Chalaya Nadezhda M. Zoteyeva Alena A. Gurina Mariya A. Kuznetsova Miles Armstrong Ingo Hein Polina E. Drobyazina Emil E. Khavkin Elena V. Rogozina Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars
description	(1) Background: Although resistance to pathogens and pests has been researched in many potato cultivars and breeding lines with DNA markers, there is scarce evidence as to the efficiency of the marker-assisted selection (MAS) for these traits when applied at the early stages of breeding. A goal of this study was to estimate the potential of affordable DNA markers to track resistance genes that are effective against the pathogen <i>Phytophthora infestans</i> (<i>Rpi</i> genes)<i>,</i> as a practical breeding tool on a progeny of 68 clones derived from a cross between the cultivar Sudarynya and the hybrid 13/11-09. (2) Methods: this population was studied for four years to elucidate the distribution of late blight (LB) resistance and other agronomical desirable or simple to phenotype traits such as tuber and flower pigmentation, yield capacity and structure. LB resistance was phenotypically evaluated following natural and artificial infection and the presence/absence of nine <i>Rpi</i> genes was assessed with 11 sequence-characterized amplified region (SCAR) markers. To validate this analysis, the profile of <i>Rpi</i> genes in the 13/11-09 parent was established using diagnostic resistance gene enrichment sequencing (dRenSeq) as a gold standard. (3) Results: at the early stages of a breeding program, when screening the segregation of F1 offspring, MAS can halve the workload and selected SCAR markers for <i>Rpi</i> genes provide useful tools.
format	article
author	Mariya P. Beketova Nadezhda A. Chalaya Nadezhda M. Zoteyeva Alena A. Gurina Mariya A. Kuznetsova Miles Armstrong Ingo Hein Polina E. Drobyazina Emil E. Khavkin Elena V. Rogozina
author_facet	Mariya P. Beketova Nadezhda A. Chalaya Nadezhda M. Zoteyeva Alena A. Gurina Mariya A. Kuznetsova Miles Armstrong Ingo Hein Polina E. Drobyazina Emil E. Khavkin Elena V. Rogozina
author_sort	Mariya P. Beketova
title	Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars
title_short	Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars
title_full	Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars
title_fullStr	Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars
title_full_unstemmed	Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars
title_sort	combination breeding and marker-assisted selection to develop late blight resistant potato cultivars
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8d87a950df2d4f98848189c6209eb2a0
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Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars

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