Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections

Abstract Frost stress is one of the major abiotic stresses causing seedling death and yield reduction in winter pea. To improve the frost tolerance of pea, field evaluation of frost tolerance was conducted on 672 diverse pea accessions at three locations in Northern China in three growing seasons fr...

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Autores principales:	Rong Liu, Li Fang, Tao Yang, Xiaoyan Zhang, Jinguo Hu, Hongyan Zhang, Wenliang Han, Zeke Hua, Junjie Hao, Xuxiao Zong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d8a7c8b06724a5b88e38fb635d891c9
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spelling	oai:doaj.org-article:8d8a7c8b06724a5b88e38fb635d891c92021-12-02T16:06:44ZMarker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections10.1038/s41598-017-06222-y2045-2322https://doaj.org/article/8d8a7c8b06724a5b88e38fb635d891c92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06222-yhttps://doaj.org/toc/2045-2322Abstract Frost stress is one of the major abiotic stresses causing seedling death and yield reduction in winter pea. To improve the frost tolerance of pea, field evaluation of frost tolerance was conducted on 672 diverse pea accessions at three locations in Northern China in three growing seasons from 2013 to 2016 and marker-trait association analysis of frost tolerance were performed with 267 informative SSR markers in this study. Sixteen accessions were identified as the most winter-hardy for their ability to survive in all nine field experiments with a mean survival rate of 0.57, ranging from 0.41 to 0.75. Population structure analysis revealed a structured population of two sub-populations plus some admixtures in the 672 accessions. Association analysis detected seven markers that repeatedly had associations with frost tolerance in at least two different environments with two different statistical models. One of the markers is the functional marker EST1109 on LG VI which was predicted to co-localize with a gene involved in the metabolism of glycoproteins in response to chilling stress and may provide a novel mechanism of frost tolerance in pea. These winter-hardy germplasms and frost tolerance associated markers will play a vital role in marker-assisted breeding for winter-hardy pea cultivar.Rong LiuLi FangTao YangXiaoyan ZhangJinguo HuHongyan ZhangWenliang HanZeke HuaJunjie HaoXuxiao ZongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rong Liu Li Fang Tao Yang Xiaoyan Zhang Jinguo Hu Hongyan Zhang Wenliang Han Zeke Hua Junjie Hao Xuxiao Zong Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections
description	Abstract Frost stress is one of the major abiotic stresses causing seedling death and yield reduction in winter pea. To improve the frost tolerance of pea, field evaluation of frost tolerance was conducted on 672 diverse pea accessions at three locations in Northern China in three growing seasons from 2013 to 2016 and marker-trait association analysis of frost tolerance were performed with 267 informative SSR markers in this study. Sixteen accessions were identified as the most winter-hardy for their ability to survive in all nine field experiments with a mean survival rate of 0.57, ranging from 0.41 to 0.75. Population structure analysis revealed a structured population of two sub-populations plus some admixtures in the 672 accessions. Association analysis detected seven markers that repeatedly had associations with frost tolerance in at least two different environments with two different statistical models. One of the markers is the functional marker EST1109 on LG VI which was predicted to co-localize with a gene involved in the metabolism of glycoproteins in response to chilling stress and may provide a novel mechanism of frost tolerance in pea. These winter-hardy germplasms and frost tolerance associated markers will play a vital role in marker-assisted breeding for winter-hardy pea cultivar.
format	article
author	Rong Liu Li Fang Tao Yang Xiaoyan Zhang Jinguo Hu Hongyan Zhang Wenliang Han Zeke Hua Junjie Hao Xuxiao Zong
author_facet	Rong Liu Li Fang Tao Yang Xiaoyan Zhang Jinguo Hu Hongyan Zhang Wenliang Han Zeke Hua Junjie Hao Xuxiao Zong
author_sort	Rong Liu
title	Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections
title_short	Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections
title_full	Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections
title_fullStr	Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections
title_full_unstemmed	Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections
title_sort	marker-trait association analysis of frost tolerance of 672 worldwide pea (pisum sativum l.) collections
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8d8a7c8b06724a5b88e38fb635d891c9
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Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections

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