Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.

<h4>Objectives</h4>Epidemic methicillin-resistant S. aureus (MRSA) clones cause infections in both hospital and community settings. As a biofilm phenotype further facilitates evasion of the host immune system and antibiotics, we compared the biofilm-forming capacities of various MRSA clo...

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Autores principales:	Evelyn Vanhommerig, Pieter Moons, Daniel Pirici, Christine Lammens, Jean-Pierre Hernalsteens, Henri De Greve, Samir Kumar-Singh, Herman Goossens, Surbhi Malhotra-Kumar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d93c1b6679c408cb1adf556d0084a80
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spelling	oai:doaj.org-article:8d93c1b6679c408cb1adf556d0084a802021-11-25T06:05:31ZComparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.1932-620310.1371/journal.pone.0104561https://doaj.org/article/8d93c1b6679c408cb1adf556d0084a802014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25105505/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objectives</h4>Epidemic methicillin-resistant S. aureus (MRSA) clones cause infections in both hospital and community settings. As a biofilm phenotype further facilitates evasion of the host immune system and antibiotics, we compared the biofilm-forming capacities of various MRSA clones.<h4>Methods</h4>Seventy-six MRSA classified into 13 clones (USA300, EMRSA-15, Hungarian/Brazilian etc.), and isolated from infections or from carriers were studied for biofilm formation under static and dynamic conditions. Static biofilms in microtitre plates were quantified colorimetrically. Dynamic biofilms (Bioflux 200, Fluxion, USA) were studied by confocal laser-scanning and time-lapse microscopy, and the total volume occupied by live/dead bacteria quantified by Volocity 5.4.1 (Improvision, UK).<h4>Results</h4>MRSA harbouring SCCmec IV produced significantly more biomass under static conditions than SCCmec I-III (P = 0.003), and those harbouring SCCmec II significantly less than those harbouring SCCmec I or III (P<0.001). In the dynamic model, SCCmec I-III harbouring MRSA were significantly better biofilm formers than SCCmec IV (P = 0.036). Only 16 strains successfully formed biofilms under both conditions, of which 13 harboured SCCmec IV and included all tested USA300 strains (n = 3). However, USA300 demonstrated remarkably lower percentages of cell-occupied space (6.6%) compared to the other clones (EMRSA-15 = 19.0%) under dynamic conditions. Time-lapse microscopy of dynamic biofilms demonstrated that USA300 formed long viscoelastic tethers that stretched far from the point of attachment, while EMRSA-15 consisted of micro-colonies attached densely to the surface.<h4>Conclusions</h4>MRSA harbouring SCCmec types IV and I-III demonstrate distinct biofilm forming capacities, possibly owing to their adaptation to the community and hospital settings, respectively. USA300 demonstrated abundant biofilm formation under both conditions, which probably confers a competitive advantage, contributing to its remarkable success as a pathogen.Evelyn VanhommerigPieter MoonsDaniel PiriciChristine LammensJean-Pierre HernalsteensHenri De GreveSamir Kumar-SinghHerman GoossensSurbhi Malhotra-KumarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104561 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evelyn Vanhommerig Pieter Moons Daniel Pirici Christine Lammens Jean-Pierre Hernalsteens Henri De Greve Samir Kumar-Singh Herman Goossens Surbhi Malhotra-Kumar Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.
description	<h4>Objectives</h4>Epidemic methicillin-resistant S. aureus (MRSA) clones cause infections in both hospital and community settings. As a biofilm phenotype further facilitates evasion of the host immune system and antibiotics, we compared the biofilm-forming capacities of various MRSA clones.<h4>Methods</h4>Seventy-six MRSA classified into 13 clones (USA300, EMRSA-15, Hungarian/Brazilian etc.), and isolated from infections or from carriers were studied for biofilm formation under static and dynamic conditions. Static biofilms in microtitre plates were quantified colorimetrically. Dynamic biofilms (Bioflux 200, Fluxion, USA) were studied by confocal laser-scanning and time-lapse microscopy, and the total volume occupied by live/dead bacteria quantified by Volocity 5.4.1 (Improvision, UK).<h4>Results</h4>MRSA harbouring SCCmec IV produced significantly more biomass under static conditions than SCCmec I-III (P = 0.003), and those harbouring SCCmec II significantly less than those harbouring SCCmec I or III (P<0.001). In the dynamic model, SCCmec I-III harbouring MRSA were significantly better biofilm formers than SCCmec IV (P = 0.036). Only 16 strains successfully formed biofilms under both conditions, of which 13 harboured SCCmec IV and included all tested USA300 strains (n = 3). However, USA300 demonstrated remarkably lower percentages of cell-occupied space (6.6%) compared to the other clones (EMRSA-15 = 19.0%) under dynamic conditions. Time-lapse microscopy of dynamic biofilms demonstrated that USA300 formed long viscoelastic tethers that stretched far from the point of attachment, while EMRSA-15 consisted of micro-colonies attached densely to the surface.<h4>Conclusions</h4>MRSA harbouring SCCmec types IV and I-III demonstrate distinct biofilm forming capacities, possibly owing to their adaptation to the community and hospital settings, respectively. USA300 demonstrated abundant biofilm formation under both conditions, which probably confers a competitive advantage, contributing to its remarkable success as a pathogen.
format	article
author	Evelyn Vanhommerig Pieter Moons Daniel Pirici Christine Lammens Jean-Pierre Hernalsteens Henri De Greve Samir Kumar-Singh Herman Goossens Surbhi Malhotra-Kumar
author_facet	Evelyn Vanhommerig Pieter Moons Daniel Pirici Christine Lammens Jean-Pierre Hernalsteens Henri De Greve Samir Kumar-Singh Herman Goossens Surbhi Malhotra-Kumar
author_sort	Evelyn Vanhommerig
title	Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.
title_short	Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.
title_full	Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.
title_fullStr	Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.
title_full_unstemmed	Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.
title_sort	comparison of biofilm formation between major clonal lineages of methicillin resistant staphylococcus aureus.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/8d93c1b6679c408cb1adf556d0084a80
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Comparison of biofilm formation between major clonal lineages of methicillin resistant Staphylococcus aureus.

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