Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer

Abstract The detection of circulating cell-free DNA (cfDNA) by liquid biopsy is reported to provide prognostic information in colorectal cancer (CRC). Although the frequency of BRAF V600E mutation in CRC is less than 10%, it is associated with poor responses to conventional chemotherapy. We conducte...

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Autores principales: Keita Tanaka, Yoichiro Yoshida, Teppei Yamada, Takaomi Hayashi, Hideki Shimaoka, Fumihiro Yoshimura, Ryuji Kajitani, Taro Munechika, Yoshiko Matsumoto, Hideki Nagano, Akira Komono, Ryohei Sakamoto, Ryo Nakashima, Naoya Aisu, Gumpei Yoshimatsu, Suguru Hasegawa
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spelling oai:doaj.org-article:8d9438e0d8b04f329885f76dfce1bab62021-12-02T18:02:50ZOncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer10.1038/s41598-021-92795-82045-2322https://doaj.org/article/8d9438e0d8b04f329885f76dfce1bab62021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92795-8https://doaj.org/toc/2045-2322Abstract The detection of circulating cell-free DNA (cfDNA) by liquid biopsy is reported to provide prognostic information in colorectal cancer (CRC). Although the frequency of BRAF V600E mutation in CRC is less than 10%, it is associated with poor responses to conventional chemotherapy. We conducted a prospective study to investigate the relationship between the perioperative mutant allele frequency (MAF) of BRAF V600E and tumor recurrence, and to evaluate the possibility of early detection of recurrence. Among 362 patients who underwent radical resection, cfDNA was extracted from the perioperative blood of 11 CRC patients with BRAF V600E mutation and analyzed using the digital polymerase chain reaction (dPCR) system. The median follow-up time was 22 months, and there were four cases of recurrence. Although there was no correlation between recurrence and the perioperative MAF of BRAF V600E, tumor diameter was correlated with the MAF (p = 0.024), and the MAF increased with time in two patients from whom additional samples were obtained prior to recurrence. In this study, we identified a correlation between the pathological tumor diameter and the MAF, but it was difficult to predict recurrence by measuring cfDNA with BRAF V600E mutation in the perioperative period of radical resection of CRC.Keita TanakaYoichiro YoshidaTeppei YamadaTakaomi HayashiHideki ShimaokaFumihiro YoshimuraRyuji KajitaniTaro MunechikaYoshiko MatsumotoHideki NaganoAkira KomonoRyohei SakamotoRyo NakashimaNaoya AisuGumpei YoshimatsuSuguru HasegawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Keita Tanaka
Yoichiro Yoshida
Teppei Yamada
Takaomi Hayashi
Hideki Shimaoka
Fumihiro Yoshimura
Ryuji Kajitani
Taro Munechika
Yoshiko Matsumoto
Hideki Nagano
Akira Komono
Ryohei Sakamoto
Ryo Nakashima
Naoya Aisu
Gumpei Yoshimatsu
Suguru Hasegawa
Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
description Abstract The detection of circulating cell-free DNA (cfDNA) by liquid biopsy is reported to provide prognostic information in colorectal cancer (CRC). Although the frequency of BRAF V600E mutation in CRC is less than 10%, it is associated with poor responses to conventional chemotherapy. We conducted a prospective study to investigate the relationship between the perioperative mutant allele frequency (MAF) of BRAF V600E and tumor recurrence, and to evaluate the possibility of early detection of recurrence. Among 362 patients who underwent radical resection, cfDNA was extracted from the perioperative blood of 11 CRC patients with BRAF V600E mutation and analyzed using the digital polymerase chain reaction (dPCR) system. The median follow-up time was 22 months, and there were four cases of recurrence. Although there was no correlation between recurrence and the perioperative MAF of BRAF V600E, tumor diameter was correlated with the MAF (p = 0.024), and the MAF increased with time in two patients from whom additional samples were obtained prior to recurrence. In this study, we identified a correlation between the pathological tumor diameter and the MAF, but it was difficult to predict recurrence by measuring cfDNA with BRAF V600E mutation in the perioperative period of radical resection of CRC.
format article
author Keita Tanaka
Yoichiro Yoshida
Teppei Yamada
Takaomi Hayashi
Hideki Shimaoka
Fumihiro Yoshimura
Ryuji Kajitani
Taro Munechika
Yoshiko Matsumoto
Hideki Nagano
Akira Komono
Ryohei Sakamoto
Ryo Nakashima
Naoya Aisu
Gumpei Yoshimatsu
Suguru Hasegawa
author_facet Keita Tanaka
Yoichiro Yoshida
Teppei Yamada
Takaomi Hayashi
Hideki Shimaoka
Fumihiro Yoshimura
Ryuji Kajitani
Taro Munechika
Yoshiko Matsumoto
Hideki Nagano
Akira Komono
Ryohei Sakamoto
Ryo Nakashima
Naoya Aisu
Gumpei Yoshimatsu
Suguru Hasegawa
author_sort Keita Tanaka
title Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
title_short Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
title_full Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
title_fullStr Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
title_full_unstemmed Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
title_sort oncological evaluation in the perioperative period using cfdna with braf v600e mutation in patients with colorectal cancer
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/8d9438e0d8b04f329885f76dfce1bab6
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