Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion

Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident...

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Autores principales:	Reinier J. de Vries, Stephanie E. J. Cronin, Padraic Romfh, Casie A. Pendexter, Rohil Jain, Benjamin T. Wilks, Siavash Raigani, Thomas M. van Gulik, Peili Chen, Heidi Yeh, Korkut Uygun, Shannon N. Tessier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8d96b9d2958643e1949f6fefe072eaea
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spelling	oai:doaj.org-article:8d96b9d2958643e1949f6fefe072eaea2021-11-04T06:49:42ZNon-invasive quantification of the mitochondrial redox state in livers during machine perfusion1932-6203https://doaj.org/article/8d96b9d2958643e1949f6fefe072eaea2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550443/?tool=EBIhttps://doaj.org/toc/1932-6203Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.Reinier J. de VriesStephanie E. J. CroninPadraic RomfhCasie A. PendexterRohil JainBenjamin T. WilksSiavash RaiganiThomas M. van GulikPeili ChenHeidi YehKorkut UygunShannon N. TessierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reinier J. de Vries Stephanie E. J. Cronin Padraic Romfh Casie A. Pendexter Rohil Jain Benjamin T. Wilks Siavash Raigani Thomas M. van Gulik Peili Chen Heidi Yeh Korkut Uygun Shannon N. Tessier Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
description	Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.
format	article
author	Reinier J. de Vries Stephanie E. J. Cronin Padraic Romfh Casie A. Pendexter Rohil Jain Benjamin T. Wilks Siavash Raigani Thomas M. van Gulik Peili Chen Heidi Yeh Korkut Uygun Shannon N. Tessier
author_facet	Reinier J. de Vries Stephanie E. J. Cronin Padraic Romfh Casie A. Pendexter Rohil Jain Benjamin T. Wilks Siavash Raigani Thomas M. van Gulik Peili Chen Heidi Yeh Korkut Uygun Shannon N. Tessier
author_sort	Reinier J. de Vries
title	Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
title_short	Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
title_full	Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
title_fullStr	Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
title_full_unstemmed	Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
title_sort	non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8d96b9d2958643e1949f6fefe072eaea
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Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion

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