Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia

Sameh Amara1, Jorge Domenech2, Faouzi Jenhani31Cellular Immunology and Cytometry, National Blood Transfusion Center, Tunis, Tunisia; 2Hematopoiesis Laboratory, Faculty of Medicine, University of Tours, Tours, France; 3Faculty of Pharmacy, Unit Research in Immunology, Tunis, TunisiaBackground: An int...

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Autores principales:	Sameh Amara, Jorge Domenech, Faouzi Jenhani
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/8d96cd3f236a417d8f7bce2004ed0be8
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spelling	oai:doaj.org-article:8d96cd3f236a417d8f7bce2004ed0be82021-12-02T01:14:10ZStromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia1179-1373https://doaj.org/article/8d96cd3f236a417d8f7bce2004ed0be82010-10-01T00:00:00Zhttp://www.dovepress.com/stromal-cell-derived-factor-1-polymorphism-in-patients-infected-with-h-a5444https://doaj.org/toc/1179-1373Sameh Amara1, Jorge Domenech2, Faouzi Jenhani31Cellular Immunology and Cytometry, National Blood Transfusion Center, Tunis, Tunisia; 2Hematopoiesis Laboratory, Faculty of Medicine, University of Tours, Tours, France; 3Faculty of Pharmacy, Unit Research in Immunology, Tunis, TunisiaBackground: An interesting finding in the epidemiology of human immunodeficiency virus (HIV) infection is that certain mutations in genes coding for chemokines, and their receptors and ligands, may confer resistance or susceptibility to HIV-1 infection and acquired immunodeficiency syndrome (AIDS) progression. The mutation most frequently studied is stromal cell-derived factor (SDF)1-3&#39;A, a single nucleotide polymorphism in the 3&#39; untranslated region at the 801 position of the SDF1 gene, which seems to be associated with susceptibility or resistance to diseases, including AIDS. We examined the frequency of the above polymorphisms in the Tunisian population, and evaluated their contribution to a protective genetic background against HIV infection and progression.Methods and materials: One hundred forty blood samples from HIV-infected patients from the Cellular Immunology Research Laboratory at the National Blood Transfusion Center were compared with those of 164 random blood donors from the same center. Genotyping was initially performed by polymerase chain reaction (PCR) analysis. SDF1 PCR product genomic regions were further subjected to restriction fragment length polymorphism analysis for genotype determination. Screening for the SDF1 polymorphism in the HIV-infected population yielded 56 heterozygous (40%), 52 mutation homozygous (37.1%), and 32 wild-type homozygous (22.8%) subjects. In contrast, in our healthy population, we found 70/164 heterozygous (42.6%), nine mutation homozygous (5.4%), and 85 wild-type homozygous (51.8%) subjects. The allele frequencies in the HIV-infected and healthy populations were f(SD1 3&#39;A) = 57.1%, f(SDF1) = 42.8%, f(SDF1 3&#39;A) = 26.8%, and f(SDF1) = 73.1%, respectively. The allelic and genotypic frequencies of the SDF1 3&#39;A in our population show significantly higher distribution profiles compared with those observed in other Caucasian, European, and African American populations. Our results were examined by X2 test and appear to confirm an association between polymorphism and AIDS progression. A higher odds ratio (&gt;1) was found for the SDF1-3&#39;A allele than for the wild-type allele (&lt;1).Conclusion: This result seems to confirm that the SDF1-3&#39;A allele is associated with acceleration and progression from HIV infection to AIDS in the Tunisian population.Keywords: human immunodeficiency virus, SDF1 polymorphism, Tunisia Sameh AmaraJorge DomenechFaouzi JenhaniDove Medical PressarticleImmunologic diseases. AllergyRC581-607ENHIV/AIDS: Research and Palliative Care, Vol 2010, Iss default, Pp 203-209 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607
spellingShingle	Immunologic diseases. Allergy RC581-607 Sameh Amara Jorge Domenech Faouzi Jenhani Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia
description	Sameh Amara1, Jorge Domenech2, Faouzi Jenhani31Cellular Immunology and Cytometry, National Blood Transfusion Center, Tunis, Tunisia; 2Hematopoiesis Laboratory, Faculty of Medicine, University of Tours, Tours, France; 3Faculty of Pharmacy, Unit Research in Immunology, Tunis, TunisiaBackground: An interesting finding in the epidemiology of human immunodeficiency virus (HIV) infection is that certain mutations in genes coding for chemokines, and their receptors and ligands, may confer resistance or susceptibility to HIV-1 infection and acquired immunodeficiency syndrome (AIDS) progression. The mutation most frequently studied is stromal cell-derived factor (SDF)1-3&#39;A, a single nucleotide polymorphism in the 3&#39; untranslated region at the 801 position of the SDF1 gene, which seems to be associated with susceptibility or resistance to diseases, including AIDS. We examined the frequency of the above polymorphisms in the Tunisian population, and evaluated their contribution to a protective genetic background against HIV infection and progression.Methods and materials: One hundred forty blood samples from HIV-infected patients from the Cellular Immunology Research Laboratory at the National Blood Transfusion Center were compared with those of 164 random blood donors from the same center. Genotyping was initially performed by polymerase chain reaction (PCR) analysis. SDF1 PCR product genomic regions were further subjected to restriction fragment length polymorphism analysis for genotype determination. Screening for the SDF1 polymorphism in the HIV-infected population yielded 56 heterozygous (40%), 52 mutation homozygous (37.1%), and 32 wild-type homozygous (22.8%) subjects. In contrast, in our healthy population, we found 70/164 heterozygous (42.6%), nine mutation homozygous (5.4%), and 85 wild-type homozygous (51.8%) subjects. The allele frequencies in the HIV-infected and healthy populations were f(SD1 3&#39;A) = 57.1%, f(SDF1) = 42.8%, f(SDF1 3&#39;A) = 26.8%, and f(SDF1) = 73.1%, respectively. The allelic and genotypic frequencies of the SDF1 3&#39;A in our population show significantly higher distribution profiles compared with those observed in other Caucasian, European, and African American populations. Our results were examined by X2 test and appear to confirm an association between polymorphism and AIDS progression. A higher odds ratio (&gt;1) was found for the SDF1-3&#39;A allele than for the wild-type allele (&lt;1).Conclusion: This result seems to confirm that the SDF1-3&#39;A allele is associated with acceleration and progression from HIV infection to AIDS in the Tunisian population.Keywords: human immunodeficiency virus, SDF1 polymorphism, Tunisia
format	article
author	Sameh Amara Jorge Domenech Faouzi Jenhani
author_facet	Sameh Amara Jorge Domenech Faouzi Jenhani
author_sort	Sameh Amara
title	Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia
title_short	Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia
title_full	Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia
title_fullStr	Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia
title_full_unstemmed	Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia
title_sort	stromal cell-derived factor 1 polymorphism in patients infected with hiv and implications for aids progression in tunisia
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/8d96cd3f236a417d8f7bce2004ed0be8
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Stromal cell-derived factor 1 polymorphism in patients infected with HIV and implications for AIDS progression in Tunisia

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