IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.

Group A Rotaviruses are the most common cause of severe, dehydrating diarrhea in children worldwide. The aim of the present work was to evaluate protection against rotavirus (RV) diarrhea conferred by the prophylactic administration of specific IgY antibodies (Ab) to gnotobiotic piglets experimental...

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Autores principales:	Celina G Vega, Marina Bok, Anastasia N Vlasova, Kuldeep S Chattha, Fernando M Fernández, Andrés Wigdorovitz, Viviana G Parreño, Linda J Saif
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8da72762f9264c369e81401701f3095a
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spelling	oai:doaj.org-article:8da72762f9264c369e81401701f3095a2021-11-18T07:09:39ZIgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.1932-620310.1371/journal.pone.0042788https://doaj.org/article/8da72762f9264c369e81401701f3095a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880110/?tool=EBIhttps://doaj.org/toc/1932-6203Group A Rotaviruses are the most common cause of severe, dehydrating diarrhea in children worldwide. The aim of the present work was to evaluate protection against rotavirus (RV) diarrhea conferred by the prophylactic administration of specific IgY antibodies (Ab) to gnotobiotic piglets experimentally inoculated with virulent Wa G1P[8] human rotavirus (HRV). Chicken egg yolk IgY Ab generated from Wa HRV hyperimmunized hens specifically recognized (ELISA) and neutralized Wa HRV in vitro. Supplementation of the RV Ab free cow milk diet with Wa HRV-specific egg yolk IgY Ab at a final ELISA Ab titer of 4096 (virus neutralization -VN- titer = 256) for 9 days conferred full protection against Wa HRV associated diarrhea and significantly reduced virus shedding. This protection was dose-dependent. The oral administration of semi-purified passive IgY Abs from chickens did not affect the isotype profile of the pig Ab secreting cell (ASC) responses to Wa HRV infection, but it was associated with significantly fewer numbers of HRV-specific IgA ASC in the duodenum. We further analyzed the pigś immune responses to the passive IgY treatment. The oral administration of IgY Abs induced IgG Ab responses to chicken IgY in serum and local IgA and IgG Ab responses to IgY in the intestinal contents of neonatal piglets in a dose dependent manner. To our knowledge, this is the first study to show that IgY Abs administered orally as a milk supplement passively protect neonatal pigs against an enteric viral pathogen (HRV). Piglets are an animal model with a gastrointestinal physiology and an immune system that closely mimic human infants. This strategy can be scaled-up to inexpensively produce large amounts of polyclonal IgY Abs from egg yolks to be applied as a preventive and therapeutic passive Ab treatment to control RV diarrhea.Celina G VegaMarina BokAnastasia N VlasovaKuldeep S ChatthaFernando M FernándezAndrés WigdorovitzViviana G ParreñoLinda J SaifPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42788 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Celina G Vega Marina Bok Anastasia N Vlasova Kuldeep S Chattha Fernando M Fernández Andrés Wigdorovitz Viviana G Parreño Linda J Saif IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.
description	Group A Rotaviruses are the most common cause of severe, dehydrating diarrhea in children worldwide. The aim of the present work was to evaluate protection against rotavirus (RV) diarrhea conferred by the prophylactic administration of specific IgY antibodies (Ab) to gnotobiotic piglets experimentally inoculated with virulent Wa G1P[8] human rotavirus (HRV). Chicken egg yolk IgY Ab generated from Wa HRV hyperimmunized hens specifically recognized (ELISA) and neutralized Wa HRV in vitro. Supplementation of the RV Ab free cow milk diet with Wa HRV-specific egg yolk IgY Ab at a final ELISA Ab titer of 4096 (virus neutralization -VN- titer = 256) for 9 days conferred full protection against Wa HRV associated diarrhea and significantly reduced virus shedding. This protection was dose-dependent. The oral administration of semi-purified passive IgY Abs from chickens did not affect the isotype profile of the pig Ab secreting cell (ASC) responses to Wa HRV infection, but it was associated with significantly fewer numbers of HRV-specific IgA ASC in the duodenum. We further analyzed the pigś immune responses to the passive IgY treatment. The oral administration of IgY Abs induced IgG Ab responses to chicken IgY in serum and local IgA and IgG Ab responses to IgY in the intestinal contents of neonatal piglets in a dose dependent manner. To our knowledge, this is the first study to show that IgY Abs administered orally as a milk supplement passively protect neonatal pigs against an enteric viral pathogen (HRV). Piglets are an animal model with a gastrointestinal physiology and an immune system that closely mimic human infants. This strategy can be scaled-up to inexpensively produce large amounts of polyclonal IgY Abs from egg yolks to be applied as a preventive and therapeutic passive Ab treatment to control RV diarrhea.
format	article
author	Celina G Vega Marina Bok Anastasia N Vlasova Kuldeep S Chattha Fernando M Fernández Andrés Wigdorovitz Viviana G Parreño Linda J Saif
author_facet	Celina G Vega Marina Bok Anastasia N Vlasova Kuldeep S Chattha Fernando M Fernández Andrés Wigdorovitz Viviana G Parreño Linda J Saif
author_sort	Celina G Vega
title	IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.
title_short	IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.
title_full	IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.
title_fullStr	IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.
title_full_unstemmed	IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.
title_sort	igy antibodies protect against human rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8da72762f9264c369e81401701f3095a
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IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model.

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