Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil

Carbapenem resistance in Acinetobacter baumannii has reached extremely high levels worldwide and class D OXA-type carbapenemases are the main associated mechanism. The aim of this study was to evaluate the phenotypic and molecular profile of clinical carbapenem-resistant A. baumannii (CRAb) isolates...

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Autores principales:	Gabriel de Paula Gollino, Bruna Machado Escobar, Ilson Dias da Silveira, Rosa Helena Robales Siqueira, Joseane Cristina Ferreira, Ana Lúcia da Costa Darini, Vanessa Bley Ribeiro
Formato:	article
Lenguaje:	PT
Publicado:	Universidade de Santa Cruz do Sul 2021
Materias:	carbapenem-resistant acinetobacter-baumannii carbapenemases oxa-23 pfge Medicine R Internal medicine RC31-1245 Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/8da9093277fb4f5f899bacc4b2607560
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spelling	oai:doaj.org-article:8da9093277fb4f5f899bacc4b26075602021-11-11T13:45:28ZMolecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil2238-336010.17058/reci.v1i1.15017https://doaj.org/article/8da9093277fb4f5f899bacc4b26075602021-10-01T00:00:00Zhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/15017https://doaj.org/toc/2238-3360Carbapenem resistance in Acinetobacter baumannii has reached extremely high levels worldwide and class D OXA-type carbapenemases are the main associated mechanism. The aim of this study was to evaluate the phenotypic and molecular profile of clinical carbapenem-resistant A. baumannii (CRAb) isolates from a southern Brazilian border region. A. baumannii species was identified by the presence of the blaOXA-51 gene and the susceptibility profile was determined by broth microdilution. The main carbapenemases were investigated by PCR and the typing of the CRAb isolates was performed by PFGE. During the study period, a total of 36 CRAb were recovered, 71.4% from respiratory tract samples from ICU patients. High level resistance to aminoglycosides and fluroquinolones were found in contrast to polymyxin B, for which all of CRAb were susceptible. The blaOXA-23 gene was present in 34 isolates and was the only one detected other than blaOXA-51. Molecular typing revealed the presence of four clonal strains, two of them endemic throughout the study period. To the best of our knowledge, our study brings the first data about resistance profile in Acinetobacter in the western border of south of Brazil and make aware of endemic clones of CRAb-producing-OXA-23 in this region of state, contributing for the construction of the national epidemiologic scenario of CRAb.Gabriel de Paula GollinoBruna Machado EscobarIlson Dias da SilveiraRosa Helena Robales SiqueiraJoseane Cristina FerreiraAna Lúcia da Costa DariniVanessa Bley RibeiroUniversidade de Santa Cruz do Sularticlecarbapenem-resistant acinetobacter-baumanniicarbapenemasesoxa-23pfgeMedicineRInternal medicineRC31-1245Infectious and parasitic diseasesRC109-216PTRevista de Epidemiologia e Controle de Infecção, Vol 11, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	PT
topic	carbapenem-resistant acinetobacter-baumannii carbapenemases oxa-23 pfge Medicine R Internal medicine RC31-1245 Infectious and parasitic diseases RC109-216
spellingShingle	carbapenem-resistant acinetobacter-baumannii carbapenemases oxa-23 pfge Medicine R Internal medicine RC31-1245 Infectious and parasitic diseases RC109-216 Gabriel de Paula Gollino Bruna Machado Escobar Ilson Dias da Silveira Rosa Helena Robales Siqueira Joseane Cristina Ferreira Ana Lúcia da Costa Darini Vanessa Bley Ribeiro Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil
description	Carbapenem resistance in Acinetobacter baumannii has reached extremely high levels worldwide and class D OXA-type carbapenemases are the main associated mechanism. The aim of this study was to evaluate the phenotypic and molecular profile of clinical carbapenem-resistant A. baumannii (CRAb) isolates from a southern Brazilian border region. A. baumannii species was identified by the presence of the blaOXA-51 gene and the susceptibility profile was determined by broth microdilution. The main carbapenemases were investigated by PCR and the typing of the CRAb isolates was performed by PFGE. During the study period, a total of 36 CRAb were recovered, 71.4% from respiratory tract samples from ICU patients. High level resistance to aminoglycosides and fluroquinolones were found in contrast to polymyxin B, for which all of CRAb were susceptible. The blaOXA-23 gene was present in 34 isolates and was the only one detected other than blaOXA-51. Molecular typing revealed the presence of four clonal strains, two of them endemic throughout the study period. To the best of our knowledge, our study brings the first data about resistance profile in Acinetobacter in the western border of south of Brazil and make aware of endemic clones of CRAb-producing-OXA-23 in this region of state, contributing for the construction of the national epidemiologic scenario of CRAb.
format	article
author	Gabriel de Paula Gollino Bruna Machado Escobar Ilson Dias da Silveira Rosa Helena Robales Siqueira Joseane Cristina Ferreira Ana Lúcia da Costa Darini Vanessa Bley Ribeiro
author_facet	Gabriel de Paula Gollino Bruna Machado Escobar Ilson Dias da Silveira Rosa Helena Robales Siqueira Joseane Cristina Ferreira Ana Lúcia da Costa Darini Vanessa Bley Ribeiro
author_sort	Gabriel de Paula Gollino
title	Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil
title_short	Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil
title_full	Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil
title_fullStr	Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil
title_full_unstemmed	Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil
title_sort	molecular epidemiology of carbapenem-resistant acinetobacter baumannii from southern brazil
publisher	Universidade de Santa Cruz do Sul
publishDate	2021
url	https://doaj.org/article/8da9093277fb4f5f899bacc4b2607560
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Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from Southern Brazil

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