Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection

Abstract Pre-transplant screening focuses on the detection of anti-HLA alloantibodies. Previous studies have shown that IFN-γ and IL-21 producing T cells are associated with the development of acute rejection (AR). The aim of this study, was to assess whether pre-transplant donor-reactive T cells an...

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Autores principales:	Aleixandra Mendoza Rojas, Teun van Gelder, Ronella de Kuiper, Derek Reijerkerk, Marian C. Clahsen-van Groningen, Dennis A. Hesselink, Carla C. Baan, Nicole M. van Besouw
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8db403cdf73c4b05a4654cd6a3cdb0e9
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spelling	oai:doaj.org-article:8db403cdf73c4b05a4654cd6a3cdb0e92021-12-02T17:23:49ZPre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection10.1038/s41598-021-91967-w2045-2322https://doaj.org/article/8db403cdf73c4b05a4654cd6a3cdb0e92021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91967-whttps://doaj.org/toc/2045-2322Abstract Pre-transplant screening focuses on the detection of anti-HLA alloantibodies. Previous studies have shown that IFN-γ and IL-21 producing T cells are associated with the development of acute rejection (AR). The aim of this study, was to assess whether pre-transplant donor-reactive T cells and/or B cells are associated with increased rejection risk. Samples from 114 kidney transplant recipients (transplanted between 2010 and 2013) were obtained pre-transplantation. The number of donor-reactive IFN-γ and IL-21 producing cells was analyzed by ELISPOT assay. The presence of donor specific antibodies (DSA) was also determined before transplantation. Numbers of donor-reactive IFN-γ producing cells were similar in patients with or without AR whereas those of IL-21 producing cells were higher in patients with AR (p = 0.03). Significantly more patients with AR [6/30(20%)] had detectable DSA compared to patients without AR [5/84(5.9%), p = 0.03]. Multivariate logistic regression showed that donor age (OR 1.06), pre-transplant DSA (OR 5.61) and positive IL-21 ELISPOT assay (OR 2.77) were independent predictors of an increased risk for the development of AR. Aside from an advanced donor-age and pre-transplant DSA, also pre-transplant donor-reactive IL-21 producing cells are associated with the development of AR after transplantation.Aleixandra Mendoza RojasTeun van GelderRonella de KuiperDerek ReijerkerkMarian C. Clahsen-van GroningenDennis A. HesselinkCarla C. BaanNicole M. van BesouwNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleixandra Mendoza Rojas Teun van Gelder Ronella de Kuiper Derek Reijerkerk Marian C. Clahsen-van Groningen Dennis A. Hesselink Carla C. Baan Nicole M. van Besouw Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection
description	Abstract Pre-transplant screening focuses on the detection of anti-HLA alloantibodies. Previous studies have shown that IFN-γ and IL-21 producing T cells are associated with the development of acute rejection (AR). The aim of this study, was to assess whether pre-transplant donor-reactive T cells and/or B cells are associated with increased rejection risk. Samples from 114 kidney transplant recipients (transplanted between 2010 and 2013) were obtained pre-transplantation. The number of donor-reactive IFN-γ and IL-21 producing cells was analyzed by ELISPOT assay. The presence of donor specific antibodies (DSA) was also determined before transplantation. Numbers of donor-reactive IFN-γ producing cells were similar in patients with or without AR whereas those of IL-21 producing cells were higher in patients with AR (p = 0.03). Significantly more patients with AR [6/30(20%)] had detectable DSA compared to patients without AR [5/84(5.9%), p = 0.03]. Multivariate logistic regression showed that donor age (OR 1.06), pre-transplant DSA (OR 5.61) and positive IL-21 ELISPOT assay (OR 2.77) were independent predictors of an increased risk for the development of AR. Aside from an advanced donor-age and pre-transplant DSA, also pre-transplant donor-reactive IL-21 producing cells are associated with the development of AR after transplantation.
format	article
author	Aleixandra Mendoza Rojas Teun van Gelder Ronella de Kuiper Derek Reijerkerk Marian C. Clahsen-van Groningen Dennis A. Hesselink Carla C. Baan Nicole M. van Besouw
author_facet	Aleixandra Mendoza Rojas Teun van Gelder Ronella de Kuiper Derek Reijerkerk Marian C. Clahsen-van Groningen Dennis A. Hesselink Carla C. Baan Nicole M. van Besouw
author_sort	Aleixandra Mendoza Rojas
title	Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection
title_short	Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection
title_full	Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection
title_fullStr	Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection
title_full_unstemmed	Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection
title_sort	pre-transplant donor-reactive il-21 producing t cells as a tool to identify an increased risk for acute rejection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8db403cdf73c4b05a4654cd6a3cdb0e9
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Pre-transplant donor-reactive IL-21 producing T cells as a tool to identify an increased risk for acute rejection

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