Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters

Abstract Transgenic sexing strains (TSS) that carry conditional female lethal genes are advantageous for genetic control programs based on the sterile insect technique (SIT). It is desirable if females die early in development as larval diet is a major cost for mass production facilities. This can b...

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Autores principales:	Ying Yan, Rebecca J. Linger, Maxwell J. Scott
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8db48796cc434424b7070f1245e151fe
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spelling	oai:doaj.org-article:8db48796cc434424b7070f1245e151fe2021-12-02T12:32:38ZBuilding early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters10.1038/s41598-017-02763-42045-2322https://doaj.org/article/8db48796cc434424b7070f1245e151fe2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02763-4https://doaj.org/toc/2045-2322Abstract Transgenic sexing strains (TSS) that carry conditional female lethal genes are advantageous for genetic control programs based on the sterile insect technique (SIT). It is desirable if females die early in development as larval diet is a major cost for mass production facilities. This can be achieved by using a gene promoter that is only active in embryos to drive expression of the tetracycline transactivator (tTA), the transcription factor commonly used in two-component TSS. While an embryo-specific promoter is ideal it may not be essential for assembling an effective TSS as tTA can be repressed by addition of tetracycline to the diet at larval and/or adult stages. Here we have investigated this idea by isolating and employing the promoters from the Lucilia spitting image and actin 5C genes to drive tTA expression in embryos and later stages. L. cuprina TSS with the tTA drivers and tTA-regulated tetO-Lshid effectors produced only females when raised on a limited tetracycline diet. The Lshid transgene contains a sex-specific intron and as a consequence only females produce LsHID protein. TSS females died at early larval stages, which makes the lines advantageous for an SIT program.Ying YanRebecca J. LingerMaxwell J. ScottNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying Yan Rebecca J. Linger Maxwell J. Scott Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters
description	Abstract Transgenic sexing strains (TSS) that carry conditional female lethal genes are advantageous for genetic control programs based on the sterile insect technique (SIT). It is desirable if females die early in development as larval diet is a major cost for mass production facilities. This can be achieved by using a gene promoter that is only active in embryos to drive expression of the tetracycline transactivator (tTA), the transcription factor commonly used in two-component TSS. While an embryo-specific promoter is ideal it may not be essential for assembling an effective TSS as tTA can be repressed by addition of tetracycline to the diet at larval and/or adult stages. Here we have investigated this idea by isolating and employing the promoters from the Lucilia spitting image and actin 5C genes to drive tTA expression in embryos and later stages. L. cuprina TSS with the tTA drivers and tTA-regulated tetO-Lshid effectors produced only females when raised on a limited tetracycline diet. The Lshid transgene contains a sex-specific intron and as a consequence only females produce LsHID protein. TSS females died at early larval stages, which makes the lines advantageous for an SIT program.
format	article
author	Ying Yan Rebecca J. Linger Maxwell J. Scott
author_facet	Ying Yan Rebecca J. Linger Maxwell J. Scott
author_sort	Ying Yan
title	Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters
title_short	Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters
title_full	Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters
title_fullStr	Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters
title_full_unstemmed	Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters
title_sort	building early-larval sexing systems for genetic control of the australian sheep blow fly lucilia cuprina using two constitutive promoters
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8db48796cc434424b7070f1245e151fe
work_keys_str_mv	AT yingyan buildingearlylarvalsexingsystemsforgeneticcontroloftheaustraliansheepblowflyluciliacuprinausingtwoconstitutivepromoters AT rebeccajlinger buildingearlylarvalsexingsystemsforgeneticcontroloftheaustraliansheepblowflyluciliacuprinausingtwoconstitutivepromoters AT maxwelljscott buildingearlylarvalsexingsystemsforgeneticcontroloftheaustraliansheepblowflyluciliacuprinausingtwoconstitutivepromoters
_version_	1718393991208108032

Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters

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