mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics

ABSTRACT Lucy Glover’s research focuses on the role of DNA repair and recombination in antigenic variation in the parasite Trypanosoma brucei, the causative agent of both human and animal African trypanosomiasis. In this mSphere of Influence article, she reflects on how “A CRISPR-based approach for...

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Autor principal:	Lucy Glover
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Trypanosoma brucei antigenic variation VSG DNA repair and recombination CRISPR-Cas DNA repair Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8db6244baa2c43bf9dfd4872bbceadda
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spelling	oai:doaj.org-article:8db6244baa2c43bf9dfd4872bbceadda2021-11-15T15:27:54ZmSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics10.1128/mSphere.00001-202379-5042https://doaj.org/article/8db6244baa2c43bf9dfd4872bbceadda2020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00001-20https://doaj.org/toc/2379-5042ABSTRACT Lucy Glover’s research focuses on the role of DNA repair and recombination in antigenic variation in the parasite Trypanosoma brucei, the causative agent of both human and animal African trypanosomiasis. In this mSphere of Influence article, she reflects on how “A CRISPR-based approach for proteomic analysis of a single genomic locus” by Z. J. Waldrip, S. D. Byrum, A. J. Storey, J. Gao, et al. (Epigenetics 9:1207–1211, 2014, https://doi.org/10.4161/epi.29919) made an impact on her research by taking the precision of CRISPR-Cas9 and repurposing it to look at single-locus proteomics. By using this technology in trypanosomes, Dr. Glover and her colleagues could study the dynamic accumulation of repair proteins after specific damage and gain insight into how the location of a double-strand break (DSB) dictates repair pathway choice and how this may influence immune evasion in these parasites.Lucy GloverAmerican Society for MicrobiologyarticleTrypanosoma bruceiantigenic variationVSGDNA repair and recombinationCRISPR-CasDNA repairMicrobiologyQR1-502ENmSphere, Vol 5, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trypanosoma brucei antigenic variation VSG DNA repair and recombination CRISPR-Cas DNA repair Microbiology QR1-502
spellingShingle	Trypanosoma brucei antigenic variation VSG DNA repair and recombination CRISPR-Cas DNA repair Microbiology QR1-502 Lucy Glover mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics
description	ABSTRACT Lucy Glover’s research focuses on the role of DNA repair and recombination in antigenic variation in the parasite Trypanosoma brucei, the causative agent of both human and animal African trypanosomiasis. In this mSphere of Influence article, she reflects on how “A CRISPR-based approach for proteomic analysis of a single genomic locus” by Z. J. Waldrip, S. D. Byrum, A. J. Storey, J. Gao, et al. (Epigenetics 9:1207–1211, 2014, https://doi.org/10.4161/epi.29919) made an impact on her research by taking the precision of CRISPR-Cas9 and repurposing it to look at single-locus proteomics. By using this technology in trypanosomes, Dr. Glover and her colleagues could study the dynamic accumulation of repair proteins after specific damage and gain insight into how the location of a double-strand break (DSB) dictates repair pathway choice and how this may influence immune evasion in these parasites.
format	article
author	Lucy Glover
author_facet	Lucy Glover
author_sort	Lucy Glover
title	mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics
title_short	mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics
title_full	mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics
title_fullStr	mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics
title_full_unstemmed	mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics
title_sort	msphere of influence: expanding the crispr sphere with single-locus proteomics
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/8db6244baa2c43bf9dfd4872bbceadda
work_keys_str_mv	AT lucyglover msphereofinfluenceexpandingthecrisprspherewithsinglelocusproteomics
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mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics

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