Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration.
Signal-induced transcript isoform variation (TIV) includes alternative promoter usage as well as alternative splicing and alternative polyadenylation of mRNA. To assess the phenotypic relevance of signal-induced TIV, we employed exon arrays and breast epithelial cells, which migrate in response to t...
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2013
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oai:doaj.org-article:8dbbe8b824c347ce9935b0151b528bff2021-11-18T08:43:12ZEpidermal growth-factor-induced transcript isoform variation drives mammary cell migration.1932-620310.1371/journal.pone.0080566https://doaj.org/article/8dbbe8b824c347ce9935b0151b528bff2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324612/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Signal-induced transcript isoform variation (TIV) includes alternative promoter usage as well as alternative splicing and alternative polyadenylation of mRNA. To assess the phenotypic relevance of signal-induced TIV, we employed exon arrays and breast epithelial cells, which migrate in response to the epidermal growth factor (EGF). We show that EGF rapidly--within one hour--induces widespread TIV in a significant fraction of the transcriptome. Importantly, TIV characterizes many genes that display no differential expression upon stimulus. In addition, similar EGF-dependent changes are shared by a panel of mammary cell lines. A functional screen, which utilized isoform-specific siRNA oligonucleotides, indicated that several isoforms play essential, non-redundant roles in EGF-induced mammary cell migration. Taken together, our findings highlight the importance of TIV in the rapid evolvement of a phenotypic response to extracellular signals.Wolfgang J KöstlerAmit ZeiselCindy KörnerJonathan M TsaiJasmine Jacob-HirschNir Ben-ChetritKirti SharmaHadas Cohen-DvashiAssif YitzhakyEric LaderUlrich TschulenaGideon RechaviEytan DomanyStefan WiemannYosef YardenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e80566 (2013) |
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Medicine R Science Q Wolfgang J Köstler Amit Zeisel Cindy Körner Jonathan M Tsai Jasmine Jacob-Hirsch Nir Ben-Chetrit Kirti Sharma Hadas Cohen-Dvashi Assif Yitzhaky Eric Lader Ulrich Tschulena Gideon Rechavi Eytan Domany Stefan Wiemann Yosef Yarden Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration. |
| description |
Signal-induced transcript isoform variation (TIV) includes alternative promoter usage as well as alternative splicing and alternative polyadenylation of mRNA. To assess the phenotypic relevance of signal-induced TIV, we employed exon arrays and breast epithelial cells, which migrate in response to the epidermal growth factor (EGF). We show that EGF rapidly--within one hour--induces widespread TIV in a significant fraction of the transcriptome. Importantly, TIV characterizes many genes that display no differential expression upon stimulus. In addition, similar EGF-dependent changes are shared by a panel of mammary cell lines. A functional screen, which utilized isoform-specific siRNA oligonucleotides, indicated that several isoforms play essential, non-redundant roles in EGF-induced mammary cell migration. Taken together, our findings highlight the importance of TIV in the rapid evolvement of a phenotypic response to extracellular signals. |
| format |
article |
| author |
Wolfgang J Köstler Amit Zeisel Cindy Körner Jonathan M Tsai Jasmine Jacob-Hirsch Nir Ben-Chetrit Kirti Sharma Hadas Cohen-Dvashi Assif Yitzhaky Eric Lader Ulrich Tschulena Gideon Rechavi Eytan Domany Stefan Wiemann Yosef Yarden |
| author_facet |
Wolfgang J Köstler Amit Zeisel Cindy Körner Jonathan M Tsai Jasmine Jacob-Hirsch Nir Ben-Chetrit Kirti Sharma Hadas Cohen-Dvashi Assif Yitzhaky Eric Lader Ulrich Tschulena Gideon Rechavi Eytan Domany Stefan Wiemann Yosef Yarden |
| author_sort |
Wolfgang J Köstler |
| title |
Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration. |
| title_short |
Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration. |
| title_full |
Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration. |
| title_fullStr |
Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration. |
| title_full_unstemmed |
Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration. |
| title_sort |
epidermal growth-factor-induced transcript isoform variation drives mammary cell migration. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/8dbbe8b824c347ce9935b0151b528bff |
| work_keys_str_mv |
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| _version_ |
1718421394661834752 |