Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels

Abstract Atherosclerosis is a long-term disease process of the vascular system that is characterized by the formation of atherosclerotic plaques, which are inflammatory regions on medium and large-sized arteries. There are many factors contributing to plaque formation, such as changes in shear stres...

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Autores principales:	Semra Zuhal Birol, Rana Fucucuoglu, Sertac Cadirci, Ayca Sayi-Yazgan, Levent Trabzon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8dbf8ce15b874f12b2d724baed516b9c
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spelling	oai:doaj.org-article:8dbf8ce15b874f12b2d724baed516b9c2021-12-02T15:33:12ZStudying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels10.1038/s41598-021-93935-w2045-2322https://doaj.org/article/8dbf8ce15b874f12b2d724baed516b9c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93935-whttps://doaj.org/toc/2045-2322Abstract Atherosclerosis is a long-term disease process of the vascular system that is characterized by the formation of atherosclerotic plaques, which are inflammatory regions on medium and large-sized arteries. There are many factors contributing to plaque formation, such as changes in shear stress levels, rupture of endothelial cells, accumulation of lipids, and recruitment of leukocytes. Shear stress is one of the main factors that regulates the homeostasis of the circulatory system; therefore, sudden and chronic changes in shear stress may cause severe pathological conditions. In this study, microfluidic channels with cavitations were designed to mimic the shape of the atherosclerotic blood vessel, where the shear stress and pressure difference depend on design of the microchannels. Changes in the inflammatory-related molecules ICAM-1 and IL-8 were investigated in THP-1 cells in response to applied shear stresses in an continuous cycling system through microfluidic channels with periodic cavitations. ICAM-1 mRNA expression and IL-8 release were analyzed by qRT-PCR and ELISA, respectively. Additionally, the adhesion behavior of sheared THP-1 cells to endothelial cells was examined by fluorescence microscopy. The results showed that 15 Pa shear stress significantly increases expression of ICAM-1 gene and IL-8 release in THP-1 cells, whereas it decreases the adhesion between THP-1 cells and endothelial cells.Semra Zuhal BirolRana FucucuogluSertac CadirciAyca Sayi-YazganLevent TrabzonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Semra Zuhal Birol Rana Fucucuoglu Sertac Cadirci Ayca Sayi-Yazgan Levent Trabzon Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels
description	Abstract Atherosclerosis is a long-term disease process of the vascular system that is characterized by the formation of atherosclerotic plaques, which are inflammatory regions on medium and large-sized arteries. There are many factors contributing to plaque formation, such as changes in shear stress levels, rupture of endothelial cells, accumulation of lipids, and recruitment of leukocytes. Shear stress is one of the main factors that regulates the homeostasis of the circulatory system; therefore, sudden and chronic changes in shear stress may cause severe pathological conditions. In this study, microfluidic channels with cavitations were designed to mimic the shape of the atherosclerotic blood vessel, where the shear stress and pressure difference depend on design of the microchannels. Changes in the inflammatory-related molecules ICAM-1 and IL-8 were investigated in THP-1 cells in response to applied shear stresses in an continuous cycling system through microfluidic channels with periodic cavitations. ICAM-1 mRNA expression and IL-8 release were analyzed by qRT-PCR and ELISA, respectively. Additionally, the adhesion behavior of sheared THP-1 cells to endothelial cells was examined by fluorescence microscopy. The results showed that 15 Pa shear stress significantly increases expression of ICAM-1 gene and IL-8 release in THP-1 cells, whereas it decreases the adhesion between THP-1 cells and endothelial cells.
format	article
author	Semra Zuhal Birol Rana Fucucuoglu Sertac Cadirci Ayca Sayi-Yazgan Levent Trabzon
author_facet	Semra Zuhal Birol Rana Fucucuoglu Sertac Cadirci Ayca Sayi-Yazgan Levent Trabzon
author_sort	Semra Zuhal Birol
title	Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels
title_short	Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels
title_full	Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels
title_fullStr	Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels
title_full_unstemmed	Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels
title_sort	studying dynamic stress effects on the behaviour of thp-1 cells by microfluidic channels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8dbf8ce15b874f12b2d724baed516b9c
work_keys_str_mv	AT semrazuhalbirol studyingdynamicstresseffectsonthebehaviourofthp1cellsbymicrofluidicchannels AT ranafucucuoglu studyingdynamicstresseffectsonthebehaviourofthp1cellsbymicrofluidicchannels AT sertaccadirci studyingdynamicstresseffectsonthebehaviourofthp1cellsbymicrofluidicchannels AT aycasayiyazgan studyingdynamicstresseffectsonthebehaviourofthp1cellsbymicrofluidicchannels AT leventtrabzon studyingdynamicstresseffectsonthebehaviourofthp1cellsbymicrofluidicchannels
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Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels

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