Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction

Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction

Fei Han,1,* Peng Zhang,2,* Yaying Sun,2 Chao Lin,1 Peng Zhao,1 Jiwu Chen2 1Shanghai East Hospital, The Institute for Biomedical Engineering and Nanoscience, Tongji University School of Medicine, Tongji University, 2Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People&...

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Autores principales: Han F, Zhang P, Sun YY, Lin C, Zhao P, Chen JW
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Nanohydroxyapatite
polycaprolactone
Nano-fiber
Tissue engineering
Anterior cruciate ligament reconstruction
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/8dc322ec306e4da2b3c0dcb308d53c8f
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spelling oai:doaj.org-article:8dc322ec306e4da2b3c0dcb308d53c8f2021-12-02T06:31:50ZHydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction1178-2013https://doaj.org/article/8dc322ec306e4da2b3c0dcb308d53c8f2015-12-01T00:00:00Zhttps://www.dovepress.com/hydroxyapatite-doped-polycaprolactone-nanofiber-membrane-improves-tend-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fei Han,1,* Peng Zhang,2,* Yaying Sun,2 Chao Lin,1 Peng Zhao,1 Jiwu Chen2 1Shanghai East Hospital, The Institute for Biomedical Engineering and Nanoscience, Tongji University School of Medicine, Tongji University, 2Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Hamstring tendon autograft is a routine graft for anterior cruciate ligament (ACL) reconstruction. However, ways of improving the healing between the tendon and bone is often overlooked in clinical practice. This issue can be addressed by using a biomimetic scaffold. Herein, a biomimetic nanofiber membrane of polycaprolactone/nanohydroxyapatite/collagen (PCL/nHAp/Col) is fabricated that mimics the composition of native bone tissue for promoting tendon–bone healing. This membrane has good cytocompatibility, allowing for osteoblast cell adhesion and growth and bone formation. As a result, MC3T3 cells reveal a higher mineralization level in PCL/nHAp/Col membrane compared with PCL membrane alone. Further in vivo studies in ACL reconstruction in a rabbit model shows that PCL/nHAp/Col-wrapped tendon may afford superior tissue integration to nonwrapped tendon in the interface between the tendon and host bone as well as improved mechanical strength. This study shows that PCL/nHAp/Col nanofiber membrane wrapping of autologous tendon is effective for improving tendon healing with host bone in ACL reconstruction. Keywords: nanohydroxyapatite, polycaprolactone, nanofiber, tissue engineering, anterior cruciate ligament reconstructionHan FZhang PSun YYLin CZhao PChen JWDove Medical PressarticleNanohydroxyapatitepolycaprolactoneNano-fiberTissue engineeringAnterior cruciate ligament reconstructionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7333-7343 (2015)
institution DOAJ
collection DOAJ
language EN
topic Nanohydroxyapatite
polycaprolactone
Nano-fiber
Tissue engineering
Anterior cruciate ligament reconstruction
Medicine (General)
R5-920
spellingShingle Nanohydroxyapatite
polycaprolactone
Nano-fiber
Tissue engineering
Anterior cruciate ligament reconstruction
Medicine (General)
R5-920
Han F
Zhang P
Sun YY
Lin C
Zhao P
Chen JW
Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction
description Fei Han,1,* Peng Zhang,2,* Yaying Sun,2 Chao Lin,1 Peng Zhao,1 Jiwu Chen2 1Shanghai East Hospital, The Institute for Biomedical Engineering and Nanoscience, Tongji University School of Medicine, Tongji University, 2Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Hamstring tendon autograft is a routine graft for anterior cruciate ligament (ACL) reconstruction. However, ways of improving the healing between the tendon and bone is often overlooked in clinical practice. This issue can be addressed by using a biomimetic scaffold. Herein, a biomimetic nanofiber membrane of polycaprolactone/nanohydroxyapatite/collagen (PCL/nHAp/Col) is fabricated that mimics the composition of native bone tissue for promoting tendon–bone healing. This membrane has good cytocompatibility, allowing for osteoblast cell adhesion and growth and bone formation. As a result, MC3T3 cells reveal a higher mineralization level in PCL/nHAp/Col membrane compared with PCL membrane alone. Further in vivo studies in ACL reconstruction in a rabbit model shows that PCL/nHAp/Col-wrapped tendon may afford superior tissue integration to nonwrapped tendon in the interface between the tendon and host bone as well as improved mechanical strength. This study shows that PCL/nHAp/Col nanofiber membrane wrapping of autologous tendon is effective for improving tendon healing with host bone in ACL reconstruction. Keywords: nanohydroxyapatite, polycaprolactone, nanofiber, tissue engineering, anterior cruciate ligament reconstruction
format article
author Han F
Zhang P
Sun YY
Lin C
Zhao P
Chen JW
author_facet Han F
Zhang P
Sun YY
Lin C
Zhao P
Chen JW
author_sort Han F
title Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction
title_short Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction
title_full Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction
title_fullStr Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction
title_full_unstemmed Hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction
title_sort hydroxyapatite-doped polycaprolactone nanofiber membrane improves tendon–bone interface healing for anterior cruciate ligament reconstruction
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/8dc322ec306e4da2b3c0dcb308d53c8f
work_keys_str_mv AT hanf hydroxyapatitedopedpolycaprolactonenanofibermembraneimprovestendonndashboneinterfacehealingforanteriorcruciateligamentreconstruction
AT zhangp hydroxyapatitedopedpolycaprolactonenanofibermembraneimprovestendonndashboneinterfacehealingforanteriorcruciateligamentreconstruction
AT sunyy hydroxyapatitedopedpolycaprolactonenanofibermembraneimprovestendonndashboneinterfacehealingforanteriorcruciateligamentreconstruction
AT linc hydroxyapatitedopedpolycaprolactonenanofibermembraneimprovestendonndashboneinterfacehealingforanteriorcruciateligamentreconstruction
AT zhaop hydroxyapatitedopedpolycaprolactonenanofibermembraneimprovestendonndashboneinterfacehealingforanteriorcruciateligamentreconstruction
AT chenjw hydroxyapatitedopedpolycaprolactonenanofibermembraneimprovestendonndashboneinterfacehealingforanteriorcruciateligamentreconstruction
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