Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).

Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).

Direct electrical recording of the neuronal transmembrane potential has been crucial to our understanding of the biophysical mechanisms subserving neuronal computation. Existing intracellular recording techniques, however, limit the accuracy and duration of such measurements by changing intracellula...

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Autores principales: Matthew R Angle, Andreas T Schaefer
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Medicine
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Science
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Acceso en línea:https://doaj.org/article/8dc471d8bf8647f9813fd8728e8c37e7
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spelling oai:doaj.org-article:8dc471d8bf8647f9813fd8728e8c37e72021-11-18T07:08:41ZNeuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).1932-620310.1371/journal.pone.0043194https://doaj.org/article/8dc471d8bf8647f9813fd8728e8c37e72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905231/?tool=EBIhttps://doaj.org/toc/1932-6203Direct electrical recording of the neuronal transmembrane potential has been crucial to our understanding of the biophysical mechanisms subserving neuronal computation. Existing intracellular recording techniques, however, limit the accuracy and duration of such measurements by changing intracellular biochemistry and/or by damaging the plasma membrane. Here we demonstrate that nanoengineered electrodes can be used to record neuronal transmembrane potentials in brain tissue without causing these physiological perturbations. Using focused ion beam milling, we have fabricated Solid-Conductor Intracellular NanoElectrodes (SCINEs), from conventional tungsten microelectrodes. SCINEs have tips that are <300 nm in diameter for several micrometers, but can be easily handled and can be inserted into brain tissue. Performing simultaneous whole-cell patch recordings, we show that SCINEs can record action potentials (APs) as well as slower, subthreshold neuronal potentials without altering cellular properties. These results show a key role for nanotechnology in the development of new electrical recording techniques in neuroscience.Matthew R AngleAndreas T SchaeferPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43194 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Matthew R Angle
Andreas T Schaefer
Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).
description Direct electrical recording of the neuronal transmembrane potential has been crucial to our understanding of the biophysical mechanisms subserving neuronal computation. Existing intracellular recording techniques, however, limit the accuracy and duration of such measurements by changing intracellular biochemistry and/or by damaging the plasma membrane. Here we demonstrate that nanoengineered electrodes can be used to record neuronal transmembrane potentials in brain tissue without causing these physiological perturbations. Using focused ion beam milling, we have fabricated Solid-Conductor Intracellular NanoElectrodes (SCINEs), from conventional tungsten microelectrodes. SCINEs have tips that are <300 nm in diameter for several micrometers, but can be easily handled and can be inserted into brain tissue. Performing simultaneous whole-cell patch recordings, we show that SCINEs can record action potentials (APs) as well as slower, subthreshold neuronal potentials without altering cellular properties. These results show a key role for nanotechnology in the development of new electrical recording techniques in neuroscience.
format article
author Matthew R Angle
Andreas T Schaefer
author_facet Matthew R Angle
Andreas T Schaefer
author_sort Matthew R Angle
title Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).
title_short Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).
title_full Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).
title_fullStr Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).
title_full_unstemmed Neuronal recordings with solid-conductor intracellular nanoelectrodes (SCINEs).
title_sort neuronal recordings with solid-conductor intracellular nanoelectrodes (scines).
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/8dc471d8bf8647f9813fd8728e8c37e7
work_keys_str_mv AT matthewrangle neuronalrecordingswithsolidconductorintracellularnanoelectrodesscines
AT andreastschaefer neuronalrecordingswithsolidconductorintracellularnanoelectrodesscines
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