High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats

Introduction: Most infertile male are associated with poor spermatogenesis due to oxidative stress, and can be prevented with vitamin C. However, excessive amount of high dose of vitamin C can hindered and lower the sperm quality. Objective: To prove that high dose vitamin C is capable to decrease...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ratna Dwi Juniatiningrum, Taufiqurrachman Nasihun, Israhnanto Isradji
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2018
Materias:	high dose vitamin c leydig cell number sertoli cell number sperm quality. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8dc7a1c9b8a14b6f887d3908c9308ec6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8dc7a1c9b8a14b6f887d3908c9308ec6
record_format	dspace
spelling	oai:doaj.org-article:8dc7a1c9b8a14b6f887d3908c9308ec62021-11-25T13:16:02ZHigh Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats2085-15452339-093X10.26532/sainsmed.v9i1.2468https://doaj.org/article/8dc7a1c9b8a14b6f887d3908c9308ec62018-11-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/2468https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XIntroduction: Most infertile male are associated with poor spermatogenesis due to oxidative stress, and can be prevented with vitamin C. However, excessive amount of high dose of vitamin C can hindered and lower the sperm quality. Objective: To prove that high dose vitamin C is capable to decrease the number of leydig cells, sertoli cells, and sperm quality on male wistar rats. Methods: This research was using experimental method with Post Test Only Controlled Group Design. Of 24 male Wistar rats, divided randomly to 4 groups. Normal groups (Nor -G), only given 2 ml/day distilled water; vitamin C group (VC18-G, VC36-G, and VC72-G) given 18 mg/day, 36 mg/day and 72 mg/day vitamin C respectively, dissolved in 2 ml of distilled water. Sperm, the number of Leydig cells and Sertoli cells were taken from the epididymis and left right testicle on day 21. Sperm analysis using WHO standard, while the number of Leydig cells and Sertoli cells with HE staining. Results: Mann Whitney analysis indicated that the number of sperm in VC36-G and VC72-G are lower compared to that of Nor-G and VC18-G groups, p <0.05. Post-Hoc LSD analysis showed that the lowest number of Leydig and Sertoli Cell, and the weakest sperm motility and morphology in VC36-G and VC72-G groups, compared to that of Nor-G and VC18-G groups, p <0.05 . Conclusion: Vitamin C 36 and 72 mg/day were capable of reducing Leydig and Sertoli cells number, and worsen sperm quality, characterized by decreased in sperm concentration, motility and morphology in Wistar male- rats.Ratna Dwi JuniatiningrumTaufiqurrachman NasihunIsrahnanto IsradjiUniversitas Sultan Agung Semarangarticlehigh dose vitamin cleydig cell numbersertoli cell numbersperm quality.Medicine (General)R5-920ENSains Medika, Vol 9, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	high dose vitamin c leydig cell number sertoli cell number sperm quality. Medicine (General) R5-920
spellingShingle	high dose vitamin c leydig cell number sertoli cell number sperm quality. Medicine (General) R5-920 Ratna Dwi Juniatiningrum Taufiqurrachman Nasihun Israhnanto Isradji High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats
description	Introduction: Most infertile male are associated with poor spermatogenesis due to oxidative stress, and can be prevented with vitamin C. However, excessive amount of high dose of vitamin C can hindered and lower the sperm quality. Objective: To prove that high dose vitamin C is capable to decrease the number of leydig cells, sertoli cells, and sperm quality on male wistar rats. Methods: This research was using experimental method with Post Test Only Controlled Group Design. Of 24 male Wistar rats, divided randomly to 4 groups. Normal groups (Nor -G), only given 2 ml/day distilled water; vitamin C group (VC18-G, VC36-G, and VC72-G) given 18 mg/day, 36 mg/day and 72 mg/day vitamin C respectively, dissolved in 2 ml of distilled water. Sperm, the number of Leydig cells and Sertoli cells were taken from the epididymis and left right testicle on day 21. Sperm analysis using WHO standard, while the number of Leydig cells and Sertoli cells with HE staining. Results: Mann Whitney analysis indicated that the number of sperm in VC36-G and VC72-G are lower compared to that of Nor-G and VC18-G groups, p <0.05. Post-Hoc LSD analysis showed that the lowest number of Leydig and Sertoli Cell, and the weakest sperm motility and morphology in VC36-G and VC72-G groups, compared to that of Nor-G and VC18-G groups, p <0.05 . Conclusion: Vitamin C 36 and 72 mg/day were capable of reducing Leydig and Sertoli cells number, and worsen sperm quality, characterized by decreased in sperm concentration, motility and morphology in Wistar male- rats.
format	article
author	Ratna Dwi Juniatiningrum Taufiqurrachman Nasihun Israhnanto Isradji
author_facet	Ratna Dwi Juniatiningrum Taufiqurrachman Nasihun Israhnanto Isradji
author_sort	Ratna Dwi Juniatiningrum
title	High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats
title_short	High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats
title_full	High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats
title_fullStr	High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats
title_full_unstemmed	High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats
title_sort	high dose vitamin c administration effect in leydig cells, sertoli cells number, and sperm quality on male wistar rats
publisher	Universitas Sultan Agung Semarang
publishDate	2018
url	https://doaj.org/article/8dc7a1c9b8a14b6f887d3908c9308ec6
work_keys_str_mv	AT ratnadwijuniatiningrum highdosevitamincadministrationeffectinleydigcellssertolicellsnumberandspermqualityonmalewistarrats AT taufiqurrachmannasihun highdosevitamincadministrationeffectinleydigcellssertolicellsnumberandspermqualityonmalewistarrats AT israhnantoisradji highdosevitamincadministrationeffectinleydigcellssertolicellsnumberandspermqualityonmalewistarrats
_version_	1718413509132288000

High Dose Vitamin C Administration Effect In Leydig Cells, Sertoli Cells Number, And Sperm Quality On Male Wistar Rats

Ejemplares similares