Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains

Abstract The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic...

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Autores principales:	Eric Hong Jian Wong, Chow Goon Ng, Khean Lee Goh, Jamuna Vadivelu, Bow Ho, Mun Fai Loke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8dc8334fe4c94339a672209200d392de
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spelling	oai:doaj.org-article:8dc8334fe4c94339a672209200d392de2021-12-02T15:05:17ZMetabolomic analysis of low and high biofilm-forming Helicobacter pylori strains10.1038/s41598-018-19697-02045-2322https://doaj.org/article/8dc8334fe4c94339a672209200d392de2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19697-0https://doaj.org/toc/2045-2322Abstract The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic activities during biofilm formation. Our study seeks to examine the differences in metabolome of low and high biofilm-formers using the metabolomic approach. Eight H. pylori clinical strains with different biofilm-forming-capability were chosen for metabolomic analysis. Bacterial metabolites were extracted using Bligh and Dyer method and analyzed by Liquid Chromatography/Quadrupole Time-of-Flight mass spectrometry. The data was processed and analyzed using the MassHunter Qualitative Analysis and the Mass Profiler Professional programs. Based on global metabolomic profiles, low and high biofilm-formers presented as two distinctly different groups. Interestingly, low-biofilm-formers produced more metabolites than high-biofilm-formers. Further analysis was performed to identify metabolites that differed significantly (p-value < 0.005) between low and high biofilm-formers. These metabolites include major categories of lipids and metabolites involve in prostaglandin and folate metabolism. Our findings suggest that biofilm formation in H. pylori is complex and probably driven by the bacterium’ endogenous metabolism. Understanding the underlying metabolic differences between low and high biofilm-formers may enhance our current understanding of pathogenesis, extragastric survival and transmission of H. pylori infections.Eric Hong Jian WongChow Goon NgKhean Lee GohJamuna VadiveluBow HoMun Fai LokeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eric Hong Jian Wong Chow Goon Ng Khean Lee Goh Jamuna Vadivelu Bow Ho Mun Fai Loke Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
description	Abstract The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic activities during biofilm formation. Our study seeks to examine the differences in metabolome of low and high biofilm-formers using the metabolomic approach. Eight H. pylori clinical strains with different biofilm-forming-capability were chosen for metabolomic analysis. Bacterial metabolites were extracted using Bligh and Dyer method and analyzed by Liquid Chromatography/Quadrupole Time-of-Flight mass spectrometry. The data was processed and analyzed using the MassHunter Qualitative Analysis and the Mass Profiler Professional programs. Based on global metabolomic profiles, low and high biofilm-formers presented as two distinctly different groups. Interestingly, low-biofilm-formers produced more metabolites than high-biofilm-formers. Further analysis was performed to identify metabolites that differed significantly (p-value < 0.005) between low and high biofilm-formers. These metabolites include major categories of lipids and metabolites involve in prostaglandin and folate metabolism. Our findings suggest that biofilm formation in H. pylori is complex and probably driven by the bacterium’ endogenous metabolism. Understanding the underlying metabolic differences between low and high biofilm-formers may enhance our current understanding of pathogenesis, extragastric survival and transmission of H. pylori infections.
format	article
author	Eric Hong Jian Wong Chow Goon Ng Khean Lee Goh Jamuna Vadivelu Bow Ho Mun Fai Loke
author_facet	Eric Hong Jian Wong Chow Goon Ng Khean Lee Goh Jamuna Vadivelu Bow Ho Mun Fai Loke
author_sort	Eric Hong Jian Wong
title	Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_short	Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_full	Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_fullStr	Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_full_unstemmed	Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_sort	metabolomic analysis of low and high biofilm-forming helicobacter pylori strains
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8dc8334fe4c94339a672209200d392de
work_keys_str_mv	AT erichongjianwong metabolomicanalysisoflowandhighbiofilmforminghelicobacterpyloristrains AT chowgoonng metabolomicanalysisoflowandhighbiofilmforminghelicobacterpyloristrains AT kheanleegoh metabolomicanalysisoflowandhighbiofilmforminghelicobacterpyloristrains AT jamunavadivelu metabolomicanalysisoflowandhighbiofilmforminghelicobacterpyloristrains AT bowho metabolomicanalysisoflowandhighbiofilmforminghelicobacterpyloristrains AT munfailoke metabolomicanalysisoflowandhighbiofilmforminghelicobacterpyloristrains
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Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains

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