Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.

The prototype baculovirus, Autographa californica multiple nucleopolyhedrovirus, an insect pathogen, holds great potential as a gene therapy vector. To develop transductional targeting and gene delivery by baculovirus, we focused on characterizing the nature and regulation of its uptake in human can...

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Autores principales:	Johanna P Laakkonen, Anna R Mäkelä, Elina Kakkonen, Paula Turkki, Sari Kukkonen, Johan Peränen, Seppo Ylä-Herttuala, Kari J Airenne, Christian Oker-Blom, Maija Vihinen-Ranta, Varpu Marjomäki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8dd46d2115214cd08eb30b874cc70bc8
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spelling	oai:doaj.org-article:8dd46d2115214cd08eb30b874cc70bc82021-11-25T06:16:16ZClathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.1932-620310.1371/journal.pone.0005093https://doaj.org/article/8dd46d2115214cd08eb30b874cc70bc82009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19352496/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The prototype baculovirus, Autographa californica multiple nucleopolyhedrovirus, an insect pathogen, holds great potential as a gene therapy vector. To develop transductional targeting and gene delivery by baculovirus, we focused on characterizing the nature and regulation of its uptake in human cancer cells. Baculovirus entered the cells along fluid-phase markers from the raft areas into smooth-surfaced vesicles devoid of clathrin. Notably, regulators associated with macropinocytosis, namely EIPA, Pak1, Rab34, and Rac1, had no significant effect on viral transduction, and the virus did not induce fluid-phase uptake. The internalization and nuclear uptake was, however, affected by mutants of RhoA, and of Arf6, a regulator of clathrin-independent entry. Furthermore, the entry of baculovirus induced ruffle formation and triggered the uptake of fluorescent E. coli bioparticles. To conclude, baculovirus enters human cells via a clathrin-independent pathway, which is able to trigger bacterial uptake. This study increases our understanding of virus entry strategies and gives new insight into baculovirus-mediated gene delivery in human cells.Johanna P LaakkonenAnna R MäkeläElina KakkonenPaula TurkkiSari KukkonenJohan PeränenSeppo Ylä-HerttualaKari J AirenneChristian Oker-BlomMaija Vihinen-RantaVarpu MarjomäkiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5093 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johanna P Laakkonen Anna R Mäkelä Elina Kakkonen Paula Turkki Sari Kukkonen Johan Peränen Seppo Ylä-Herttuala Kari J Airenne Christian Oker-Blom Maija Vihinen-Ranta Varpu Marjomäki Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
description	The prototype baculovirus, Autographa californica multiple nucleopolyhedrovirus, an insect pathogen, holds great potential as a gene therapy vector. To develop transductional targeting and gene delivery by baculovirus, we focused on characterizing the nature and regulation of its uptake in human cancer cells. Baculovirus entered the cells along fluid-phase markers from the raft areas into smooth-surfaced vesicles devoid of clathrin. Notably, regulators associated with macropinocytosis, namely EIPA, Pak1, Rab34, and Rac1, had no significant effect on viral transduction, and the virus did not induce fluid-phase uptake. The internalization and nuclear uptake was, however, affected by mutants of RhoA, and of Arf6, a regulator of clathrin-independent entry. Furthermore, the entry of baculovirus induced ruffle formation and triggered the uptake of fluorescent E. coli bioparticles. To conclude, baculovirus enters human cells via a clathrin-independent pathway, which is able to trigger bacterial uptake. This study increases our understanding of virus entry strategies and gives new insight into baculovirus-mediated gene delivery in human cells.
format	article
author	Johanna P Laakkonen Anna R Mäkelä Elina Kakkonen Paula Turkki Sari Kukkonen Johan Peränen Seppo Ylä-Herttuala Kari J Airenne Christian Oker-Blom Maija Vihinen-Ranta Varpu Marjomäki
author_facet	Johanna P Laakkonen Anna R Mäkelä Elina Kakkonen Paula Turkki Sari Kukkonen Johan Peränen Seppo Ylä-Herttuala Kari J Airenne Christian Oker-Blom Maija Vihinen-Ranta Varpu Marjomäki
author_sort	Johanna P Laakkonen
title	Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
title_short	Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
title_full	Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
title_fullStr	Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
title_full_unstemmed	Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
title_sort	clathrin-independent entry of baculovirus triggers uptake of e. coli in non-phagocytic human cells.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/8dd46d2115214cd08eb30b874cc70bc8
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Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.

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