Protein immobilization onto electrochemically synthesized CoFe nanowires

Sri Ramulu Torati,1 Venu Reddy,1 Seok Soo Yoon,2 CheolGi Kim1 1Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea; 2Department of Physics, Andong National University, Andong, South Korea Abstract: CoFe nanowires have been synthesized...

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Autores principales:	Torati SR, Reddy V, Yoon SS, Kim CG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8de2ec147e544f23bc56d723184862c6
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spelling	oai:doaj.org-article:8de2ec147e544f23bc56d723184862c62021-12-02T05:10:41ZProtein immobilization onto electrochemically synthesized CoFe nanowires1178-2013https://doaj.org/article/8de2ec147e544f23bc56d723184862c62015-01-01T00:00:00Zhttp://www.dovepress.com/protein-immobilization-onto-electrochemically-synthesized-cofe-nanowir-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Sri Ramulu Torati,1 Venu Reddy,1 Seok Soo Yoon,2 CheolGi Kim1 1Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea; 2Department of Physics, Andong National University, Andong, South Korea Abstract: CoFe nanowires have been synthesized by the electrodeposition technique into the pores of a polycarbonate membrane with a nominal pore diameter of 50 nm, and the composition of CoFe nanowires varying by changing the source concentration of iron. The synthesized nanowire surfaces were functionalized with amine groups by treatment with aminopropyltriethoxysilane (APTES) linker, and then conjugated with streptavidin-Cy3 protein via ethyl (dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide coupling chemistry. The oxide surface of CoFe nanowire is easily modified with aminopropyltriethoxysilane to form an amine terminating group, which is covalently bonded to streptavidin-Cy3 protein. The physicochemical properties of the nanowires were analyzed through different characterization techniques such as scanning electron microscope, energy dispersive spectroscopy, and vibrating sample magnetometer. Fluorescence microscopic studies and Fourier transform infrared studies confirmed the immobilization of protein on the nanowire surface. In addition, the transmission electron microscope analysis reveals the thin protein layer which is around 12–15 nm on the nanowire surfaces. Keywords: electrodeposition, biofunctionalization, streptavidinTorati SRReddy VYoon SSKim CGDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 645-651 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Torati SR Reddy V Yoon SS Kim CG Protein immobilization onto electrochemically synthesized CoFe nanowires
description	Sri Ramulu Torati,1 Venu Reddy,1 Seok Soo Yoon,2 CheolGi Kim1 1Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea; 2Department of Physics, Andong National University, Andong, South Korea Abstract: CoFe nanowires have been synthesized by the electrodeposition technique into the pores of a polycarbonate membrane with a nominal pore diameter of 50 nm, and the composition of CoFe nanowires varying by changing the source concentration of iron. The synthesized nanowire surfaces were functionalized with amine groups by treatment with aminopropyltriethoxysilane (APTES) linker, and then conjugated with streptavidin-Cy3 protein via ethyl (dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide coupling chemistry. The oxide surface of CoFe nanowire is easily modified with aminopropyltriethoxysilane to form an amine terminating group, which is covalently bonded to streptavidin-Cy3 protein. The physicochemical properties of the nanowires were analyzed through different characterization techniques such as scanning electron microscope, energy dispersive spectroscopy, and vibrating sample magnetometer. Fluorescence microscopic studies and Fourier transform infrared studies confirmed the immobilization of protein on the nanowire surface. In addition, the transmission electron microscope analysis reveals the thin protein layer which is around 12–15 nm on the nanowire surfaces. Keywords: electrodeposition, biofunctionalization, streptavidin
format	article
author	Torati SR Reddy V Yoon SS Kim CG
author_facet	Torati SR Reddy V Yoon SS Kim CG
author_sort	Torati SR
title	Protein immobilization onto electrochemically synthesized CoFe nanowires
title_short	Protein immobilization onto electrochemically synthesized CoFe nanowires
title_full	Protein immobilization onto electrochemically synthesized CoFe nanowires
title_fullStr	Protein immobilization onto electrochemically synthesized CoFe nanowires
title_full_unstemmed	Protein immobilization onto electrochemically synthesized CoFe nanowires
title_sort	protein immobilization onto electrochemically synthesized cofe nanowires
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/8de2ec147e544f23bc56d723184862c6
work_keys_str_mv	AT toratisr proteinimmobilizationontoelectrochemicallysynthesizedcofenanowires AT reddyv proteinimmobilizationontoelectrochemicallysynthesizedcofenanowires AT yoonss proteinimmobilizationontoelectrochemicallysynthesizedcofenanowires AT kimcg proteinimmobilizationontoelectrochemicallysynthesizedcofenanowires
_version_	1718400542385897472

Protein immobilization onto electrochemically synthesized CoFe nanowires

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