Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents

Abstract Background Pineapple (Ananas comosus L. Merr.) is the third most important tropical fruit in China. In other crops, farmers can easily judge the nutritional requirements from leaf color. However, concerning pineapple, it is difficult due to the variation in leaf color of the cultivated pine...

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Autores principales: Jing Chen, Hui Zeng, Xiumei Zhang
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Lenguaje:EN
Publicado: BMC 2021
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spelling oai:doaj.org-article:8de46d9b585942d790fd28b4e8a03bc82021-11-28T12:07:26ZIntegrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents10.1186/s12870-021-03291-01471-2229https://doaj.org/article/8de46d9b585942d790fd28b4e8a03bc82021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03291-0https://doaj.org/toc/1471-2229Abstract Background Pineapple (Ananas comosus L. Merr.) is the third most important tropical fruit in China. In other crops, farmers can easily judge the nutritional requirements from leaf color. However, concerning pineapple, it is difficult due to the variation in leaf color of the cultivated pineapple varieties. A detailed understanding of the mechanisms of nutrient transport, accumulation, and assimilation was targeted in this study. We explored the D-leaf nitrogen (N), phosphorus (P), and potassium (K) contents, transcriptome, and metabolome of seven pineapple varieties. Results Significantly higher N, P, and K% contents were observed in Bali, Caine, and Golden pineapple. The transcriptome sequencing of 21 libraries resulted in the identification of 14,310 differentially expressed genes in the D-leaves of seven pineapple varieties. Genes associated with N transport and assimilation in D-leaves of pineapple was possibly regulated by nitrate and ammonium transporters, and glutamate dehydrogenases play roles in N assimilation in arginine biosynthesis pathways. Photosynthesis and photosynthesis-antenna proteins pathways were also significantly regulated between the studied genotypes. Phosphate transporters and mitochondrial phosphate transporters were differentially regulated regarding inorganic P transport. WRKY, MYB, and bHLH transcription factors were possibly regulating the phosphate transporters. The observed varying contents of K% in the D-leaves was associated to the regulation of K+ transporters and channels under the influence of Ca2+ signaling. The UPLC-MS/MS analysis detected 873 metabolites which were mainly classified as flavonoids, lipids, and phenolic acids. Conclusions These findings provide a detailed insight into the N, P, K% contents in pineapple D-leaf and their transcriptomic and metabolomic signatures.Jing ChenHui ZengXiumei ZhangBMCarticleCa2+ dependent K+ transportD-leafGS/GOGATK+ transportLeaf colorN assimilationBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-19 (2021)
institution DOAJ
collection DOAJ
language EN
topic Ca2+ dependent K+ transport
D-leaf
GS/GOGAT
K+ transport
Leaf color
N assimilation
Botany
QK1-989
spellingShingle Ca2+ dependent K+ transport
D-leaf
GS/GOGAT
K+ transport
Leaf color
N assimilation
Botany
QK1-989
Jing Chen
Hui Zeng
Xiumei Zhang
Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents
description Abstract Background Pineapple (Ananas comosus L. Merr.) is the third most important tropical fruit in China. In other crops, farmers can easily judge the nutritional requirements from leaf color. However, concerning pineapple, it is difficult due to the variation in leaf color of the cultivated pineapple varieties. A detailed understanding of the mechanisms of nutrient transport, accumulation, and assimilation was targeted in this study. We explored the D-leaf nitrogen (N), phosphorus (P), and potassium (K) contents, transcriptome, and metabolome of seven pineapple varieties. Results Significantly higher N, P, and K% contents were observed in Bali, Caine, and Golden pineapple. The transcriptome sequencing of 21 libraries resulted in the identification of 14,310 differentially expressed genes in the D-leaves of seven pineapple varieties. Genes associated with N transport and assimilation in D-leaves of pineapple was possibly regulated by nitrate and ammonium transporters, and glutamate dehydrogenases play roles in N assimilation in arginine biosynthesis pathways. Photosynthesis and photosynthesis-antenna proteins pathways were also significantly regulated between the studied genotypes. Phosphate transporters and mitochondrial phosphate transporters were differentially regulated regarding inorganic P transport. WRKY, MYB, and bHLH transcription factors were possibly regulating the phosphate transporters. The observed varying contents of K% in the D-leaves was associated to the regulation of K+ transporters and channels under the influence of Ca2+ signaling. The UPLC-MS/MS analysis detected 873 metabolites which were mainly classified as flavonoids, lipids, and phenolic acids. Conclusions These findings provide a detailed insight into the N, P, K% contents in pineapple D-leaf and their transcriptomic and metabolomic signatures.
format article
author Jing Chen
Hui Zeng
Xiumei Zhang
author_facet Jing Chen
Hui Zeng
Xiumei Zhang
author_sort Jing Chen
title Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents
title_short Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents
title_full Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents
title_fullStr Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents
title_full_unstemmed Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents
title_sort integrative transcriptomic and metabolomic analysis of d-leaf of seven pineapple varieties differing in n-p-k% contents
publisher BMC
publishDate 2021
url https://doaj.org/article/8de46d9b585942d790fd28b4e8a03bc8
work_keys_str_mv AT jingchen integrativetranscriptomicandmetabolomicanalysisofdleafofsevenpineapplevarietiesdifferinginnpkcontents
AT huizeng integrativetranscriptomicandmetabolomicanalysisofdleafofsevenpineapplevarietiesdifferinginnpkcontents
AT xiumeizhang integrativetranscriptomicandmetabolomicanalysisofdleafofsevenpineapplevarietiesdifferinginnpkcontents
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