FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.

Chloroplast formation is associated with embryo development and seedling growth. However, the relationship between chloroplast differentiation and embryo development remains unclear. Five FtsHi genes that encode proteins with high similarity to FtsH proteins, but lack Zn2+-binding motifs, are presen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaoduo Lu, Dongyuan Zhang, Shipeng Li, Yanping Su, Qiuju Liang, Hongyan Meng, Songdong Shen, Yunliu Fan, Chunming Liu, Chunyi Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8de69f931a41482fbf20084ce1fe5895
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8de69f931a41482fbf20084ce1fe5895
record_format	dspace
spelling	oai:doaj.org-article:8de69f931a41482fbf20084ce1fe58952021-11-11T08:21:31ZFtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.1932-620310.1371/journal.pone.0099741https://doaj.org/article/8de69f931a41482fbf20084ce1fe58952014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24964212/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Chloroplast formation is associated with embryo development and seedling growth. However, the relationship between chloroplast differentiation and embryo development remains unclear. Five FtsHi genes that encode proteins with high similarity to FtsH proteins, but lack Zn2+-binding motifs, are present in the Arabidopsis genome. In this study, we showed that T-DNA insertion mutations in the Arabidopsis FtsHi4 gene resulted in embryo arrest at the globular-to-heart-shaped transition stage. Transmission electron microscopic analyses revealed abnormal plastid differentiation with a severe defect in thylakoid formation in the mutant embryos. Immunocytological studies demonstrated that FtsHi4 localized in chloroplasts as a thylakoid membrane-associated protein, supporting its essential role in thylakoid membrane formation. We further showed that FtsHi4 forms protein complexes, and that there was a significant reduction in the accumulation of D2 and PsbO (two photosystem II proteins) in mutant ovules. The role of FtsHi4 in chloroplast development was confirmed using an RNA-interfering approach. Additionally, mutations in other FtsHi genes including FtsHi1, FtsHi2, and FtsHi5 caused phenotypic abnormalities similar to ftshi4 with respect to plastid differentiation during embryogenesis. Taken together, our data suggest that FtsHi4, together with FtsHi1, FtsHi2, and FtsHi5 are essential for chloroplast development in Arabidopsis.Xiaoduo LuDongyuan ZhangShipeng LiYanping SuQiuju LiangHongyan MengSongdong ShenYunliu FanChunming LiuChunyi ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99741 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoduo Lu Dongyuan Zhang Shipeng Li Yanping Su Qiuju Liang Hongyan Meng Songdong Shen Yunliu Fan Chunming Liu Chunyi Zhang FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.
description	Chloroplast formation is associated with embryo development and seedling growth. However, the relationship between chloroplast differentiation and embryo development remains unclear. Five FtsHi genes that encode proteins with high similarity to FtsH proteins, but lack Zn2+-binding motifs, are present in the Arabidopsis genome. In this study, we showed that T-DNA insertion mutations in the Arabidopsis FtsHi4 gene resulted in embryo arrest at the globular-to-heart-shaped transition stage. Transmission electron microscopic analyses revealed abnormal plastid differentiation with a severe defect in thylakoid formation in the mutant embryos. Immunocytological studies demonstrated that FtsHi4 localized in chloroplasts as a thylakoid membrane-associated protein, supporting its essential role in thylakoid membrane formation. We further showed that FtsHi4 forms protein complexes, and that there was a significant reduction in the accumulation of D2 and PsbO (two photosystem II proteins) in mutant ovules. The role of FtsHi4 in chloroplast development was confirmed using an RNA-interfering approach. Additionally, mutations in other FtsHi genes including FtsHi1, FtsHi2, and FtsHi5 caused phenotypic abnormalities similar to ftshi4 with respect to plastid differentiation during embryogenesis. Taken together, our data suggest that FtsHi4, together with FtsHi1, FtsHi2, and FtsHi5 are essential for chloroplast development in Arabidopsis.
format	article
author	Xiaoduo Lu Dongyuan Zhang Shipeng Li Yanping Su Qiuju Liang Hongyan Meng Songdong Shen Yunliu Fan Chunming Liu Chunyi Zhang
author_facet	Xiaoduo Lu Dongyuan Zhang Shipeng Li Yanping Su Qiuju Liang Hongyan Meng Songdong Shen Yunliu Fan Chunming Liu Chunyi Zhang
author_sort	Xiaoduo Lu
title	FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.
title_short	FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.
title_full	FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.
title_fullStr	FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.
title_full_unstemmed	FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.
title_sort	ftshi4 is essential for embryogenesis due to its influence on chloroplast development in arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/8de69f931a41482fbf20084ce1fe5895
work_keys_str_mv	AT xiaoduolu ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT dongyuanzhang ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT shipengli ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT yanpingsu ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT qiujuliang ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT hongyanmeng ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT songdongshen ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT yunliufan ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT chunmingliu ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis AT chunyizhang ftshi4isessentialforembryogenesisduetoitsinfluenceonchloroplastdevelopmentinarabidopsis
_version_	1718439330219819008

FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.

Ejemplares similares