Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China

Abstract Mycobacterium tuberculosis (M. tuberculosis) genotyping has dramatically improved the understanding of the epidemiology of tuberculosis (TB). In this study, 187 M. tuberculosis isolates from Xinjiang Uygur Autonomous Region (Xinjiang) and Gansu province in China were genotyped using large s...

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Autores principales:	Haixia Chen, Li He, Hairong Huang, Chengmin Shi, Xumin Ni, Guangming Dai, Liang Ma, Weimin Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8de8d54590f34bb781fcd82d8d558bf9
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spelling	oai:doaj.org-article:8de8d54590f34bb781fcd82d8d558bf92021-12-02T15:06:20ZMycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China10.1038/s41598-017-00720-92045-2322https://doaj.org/article/8de8d54590f34bb781fcd82d8d558bf92017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00720-9https://doaj.org/toc/2045-2322Abstract Mycobacterium tuberculosis (M. tuberculosis) genotyping has dramatically improved the understanding of the epidemiology of tuberculosis (TB). In this study, 187 M. tuberculosis isolates from Xinjiang Uygur Autonomous Region (Xinjiang) and Gansu province in China were genotyped using large sequence polymorphisms (LSPs) and variable number tandem repeats (VNTR). Ten isolates, which represent major nodes of VNTR-based minimum spanning tree, were selected and subsequently subjected to multi-locus sequence analyses (MLSA) that include 82 genes. Based on a robust lineage assignment, we tested the association between lineages and clinical characteristics by logistic regression. There are three major lineages of M. tuberculosis prevalent in Xinjiang, viz. the East Asian Lineage 2 (42.1%; 56/133), the Euro-American Lineage 4 (33.1%; 44/133), and the Indian and East African Lineage 3 (24.8%; 33/133); two lineages prevalent in Gansu province, which are the Lineage 2 (87%; 47/54) and the Lineage 4 (13%; 7/54). The topological structures of the MLSA-based phylogeny support the LSP-based identification of M. tuberculosis lineages. The statistical results suggest an association between the Lineage 2 and the hemoptysis/bloody sputum symptom, fever in Uygur patients. The pathogenicity of the Lineage 2 remains to be further investigated.Haixia ChenLi HeHairong HuangChengmin ShiXumin NiGuangming DaiLiang MaWeimin LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haixia Chen Li He Hairong Huang Chengmin Shi Xumin Ni Guangming Dai Liang Ma Weimin Li Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China
description	Abstract Mycobacterium tuberculosis (M. tuberculosis) genotyping has dramatically improved the understanding of the epidemiology of tuberculosis (TB). In this study, 187 M. tuberculosis isolates from Xinjiang Uygur Autonomous Region (Xinjiang) and Gansu province in China were genotyped using large sequence polymorphisms (LSPs) and variable number tandem repeats (VNTR). Ten isolates, which represent major nodes of VNTR-based minimum spanning tree, were selected and subsequently subjected to multi-locus sequence analyses (MLSA) that include 82 genes. Based on a robust lineage assignment, we tested the association between lineages and clinical characteristics by logistic regression. There are three major lineages of M. tuberculosis prevalent in Xinjiang, viz. the East Asian Lineage 2 (42.1%; 56/133), the Euro-American Lineage 4 (33.1%; 44/133), and the Indian and East African Lineage 3 (24.8%; 33/133); two lineages prevalent in Gansu province, which are the Lineage 2 (87%; 47/54) and the Lineage 4 (13%; 7/54). The topological structures of the MLSA-based phylogeny support the LSP-based identification of M. tuberculosis lineages. The statistical results suggest an association between the Lineage 2 and the hemoptysis/bloody sputum symptom, fever in Uygur patients. The pathogenicity of the Lineage 2 remains to be further investigated.
format	article
author	Haixia Chen Li He Hairong Huang Chengmin Shi Xumin Ni Guangming Dai Liang Ma Weimin Li
author_facet	Haixia Chen Li He Hairong Huang Chengmin Shi Xumin Ni Guangming Dai Liang Ma Weimin Li
author_sort	Haixia Chen
title	Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China
title_short	Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China
title_full	Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China
title_fullStr	Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China
title_full_unstemmed	Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China
title_sort	mycobacterium tuberculosis lineage distribution in xinjiang and gansu provinces, china
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8de8d54590f34bb781fcd82d8d558bf9
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_version_	1718388528150216704

Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China

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