Characterization and expression analysis of the WRKY gene family in moso bamboo

Abstract The WRKY family of transcription factors (TFs) is one of the ten largest families of TFs in higher plants and has been implicated in multiple biological processes. Here, we identified 121 WRKY TFs in moso bamboo, including five novel members that were not annotated in the Phyllostachys edul...

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Autores principales:	Long Li, Shaohua Mu, Zhanchao Cheng, Yuanwen Cheng, Ying Zhang, Ying Miao, Chenglin Hou, Xueping Li, Jian Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8dee4ed7a40f409283fa309633e1c986
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spelling	oai:doaj.org-article:8dee4ed7a40f409283fa309633e1c9862021-12-02T16:06:19ZCharacterization and expression analysis of the WRKY gene family in moso bamboo10.1038/s41598-017-06701-22045-2322https://doaj.org/article/8dee4ed7a40f409283fa309633e1c9862017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06701-2https://doaj.org/toc/2045-2322Abstract The WRKY family of transcription factors (TFs) is one of the ten largest families of TFs in higher plants and has been implicated in multiple biological processes. Here, we identified 121 WRKY TFs in moso bamboo, including five novel members that were not annotated in the Phyllostachys edulis genomic database. Estimation of the divergence time of paralogous gene pairs revealed an important role of the recent whole-genome duplication in the expansion of the WRKY family. Expression analysis based on quantitative reverse-transcription polymerase chain reaction (qRT-PCR) data revealed that a large number of PheWRKY genes varied significantly under cold or drought stress treatments, which could be defined as abiotic stress-responsive genes. The overexpression of PheWRKY72-2 in Arabidopsis resulted in a decreased sensitivity to drought stress during early seedling growth. PheWRKY72-2 may enhance plant tolerance to stress by functioning as a positive regulator of stoma closure. Our study provides a theoretical foundation and some experimental evidence for further functional verification of the PheWRKY family of TFs.Long LiShaohua MuZhanchao ChengYuanwen ChengYing ZhangYing MiaoChenglin HouXueping LiJian GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Long Li Shaohua Mu Zhanchao Cheng Yuanwen Cheng Ying Zhang Ying Miao Chenglin Hou Xueping Li Jian Gao Characterization and expression analysis of the WRKY gene family in moso bamboo
description	Abstract The WRKY family of transcription factors (TFs) is one of the ten largest families of TFs in higher plants and has been implicated in multiple biological processes. Here, we identified 121 WRKY TFs in moso bamboo, including five novel members that were not annotated in the Phyllostachys edulis genomic database. Estimation of the divergence time of paralogous gene pairs revealed an important role of the recent whole-genome duplication in the expansion of the WRKY family. Expression analysis based on quantitative reverse-transcription polymerase chain reaction (qRT-PCR) data revealed that a large number of PheWRKY genes varied significantly under cold or drought stress treatments, which could be defined as abiotic stress-responsive genes. The overexpression of PheWRKY72-2 in Arabidopsis resulted in a decreased sensitivity to drought stress during early seedling growth. PheWRKY72-2 may enhance plant tolerance to stress by functioning as a positive regulator of stoma closure. Our study provides a theoretical foundation and some experimental evidence for further functional verification of the PheWRKY family of TFs.
format	article
author	Long Li Shaohua Mu Zhanchao Cheng Yuanwen Cheng Ying Zhang Ying Miao Chenglin Hou Xueping Li Jian Gao
author_facet	Long Li Shaohua Mu Zhanchao Cheng Yuanwen Cheng Ying Zhang Ying Miao Chenglin Hou Xueping Li Jian Gao
author_sort	Long Li
title	Characterization and expression analysis of the WRKY gene family in moso bamboo
title_short	Characterization and expression analysis of the WRKY gene family in moso bamboo
title_full	Characterization and expression analysis of the WRKY gene family in moso bamboo
title_fullStr	Characterization and expression analysis of the WRKY gene family in moso bamboo
title_full_unstemmed	Characterization and expression analysis of the WRKY gene family in moso bamboo
title_sort	characterization and expression analysis of the wrky gene family in moso bamboo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8dee4ed7a40f409283fa309633e1c986
work_keys_str_mv	AT longli characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT shaohuamu characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT zhanchaocheng characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT yuanwencheng characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT yingzhang characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT yingmiao characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT chenglinhou characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT xuepingli characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo AT jiangao characterizationandexpressionanalysisofthewrkygenefamilyinmosobamboo
_version_	1718385037439664128

Characterization and expression analysis of the WRKY gene family in moso bamboo

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