Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress

To assess root metabolic differences of maize varieties in their response to lead (Pb) stress, the lead-tolerant variety Huidan No. 4 and the lead-sensitive variety Ludan No. 8 were tested under Pb-free and Pb-stressed conditions. Changes in metabolites were measured using ultra-performance liquid c...

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Autores principales:	Hanqian Zhang, Yuying Qin, Kai Huang, Fangdong Zhan, Ru Li, Jianjun Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Zea mays maize varieties metabolite Pb stress ultra-performance liquid chromatography–mass spectrometry Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/8df193f1b15846bd95bc9a819435d4df
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id	oai:doaj.org-article:8df193f1b15846bd95bc9a819435d4df
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spelling	oai:doaj.org-article:8df193f1b15846bd95bc9a819435d4df2021-11-30T11:51:16ZRoot Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress1664-462X10.3389/fpls.2021.656074https://doaj.org/article/8df193f1b15846bd95bc9a819435d4df2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.656074/fullhttps://doaj.org/toc/1664-462XTo assess root metabolic differences of maize varieties in their response to lead (Pb) stress, the lead-tolerant variety Huidan No. 4 and the lead-sensitive variety Ludan No. 8 were tested under Pb-free and Pb-stressed conditions. Changes in metabolites were measured using ultra-performance liquid chromatography–mass spectrometry. Pb stress changed the levels of the amino acids proline, glutamine, lysine, and arginine in both varieties, whereas glutamate and phenylalanine levels changed only in Huidan No. 4. Pb stress altered cystine, valine, methionine, and tryptophan levels only in Ludan No. 8. Therefore, the synthesis and decomposition of amino acids may affect the response of maize to Pb stress. The degree of change in differential metabolites for Huidan No. 4 was greater than that for Ludan No. 8. In cell wall subcellular components, increases in superoxide dismutase (SOD), peroxidases (PODs), and Pb concentrations were greater in Huidan No. 4 than in Ludan No. 8. Therefore, the greater Pb tolerance of Huidan No. 4 could be due to better sequestration of Pb in cell walls and more effective removal of reactive oxygen species (ROS) from the plant. The levels of certain metabolites only increased in Ludan No. 8, indicating that Pb-sensitive varieties may use different metabolic pathways to cope with Pb stress. Both varieties showed increased levels of some metabolites related to antioxidant protection and osmotic regulation. This study provides an understanding of maize Pb tolerance mechanisms and a basis for further development of tools for use in maize breeding.Hanqian ZhangYuying QinKai HuangFangdong ZhanRu LiRu LiJianjun ChenFrontiers Media S.A.articleZea maysmaize varietiesmetabolitePb stressultra-performance liquid chromatography–mass spectrometryPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zea mays maize varieties metabolite Pb stress ultra-performance liquid chromatography–mass spectrometry Plant culture SB1-1110
spellingShingle	Zea mays maize varieties metabolite Pb stress ultra-performance liquid chromatography–mass spectrometry Plant culture SB1-1110 Hanqian Zhang Yuying Qin Kai Huang Fangdong Zhan Ru Li Ru Li Jianjun Chen Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress
description	To assess root metabolic differences of maize varieties in their response to lead (Pb) stress, the lead-tolerant variety Huidan No. 4 and the lead-sensitive variety Ludan No. 8 were tested under Pb-free and Pb-stressed conditions. Changes in metabolites were measured using ultra-performance liquid chromatography–mass spectrometry. Pb stress changed the levels of the amino acids proline, glutamine, lysine, and arginine in both varieties, whereas glutamate and phenylalanine levels changed only in Huidan No. 4. Pb stress altered cystine, valine, methionine, and tryptophan levels only in Ludan No. 8. Therefore, the synthesis and decomposition of amino acids may affect the response of maize to Pb stress. The degree of change in differential metabolites for Huidan No. 4 was greater than that for Ludan No. 8. In cell wall subcellular components, increases in superoxide dismutase (SOD), peroxidases (PODs), and Pb concentrations were greater in Huidan No. 4 than in Ludan No. 8. Therefore, the greater Pb tolerance of Huidan No. 4 could be due to better sequestration of Pb in cell walls and more effective removal of reactive oxygen species (ROS) from the plant. The levels of certain metabolites only increased in Ludan No. 8, indicating that Pb-sensitive varieties may use different metabolic pathways to cope with Pb stress. Both varieties showed increased levels of some metabolites related to antioxidant protection and osmotic regulation. This study provides an understanding of maize Pb tolerance mechanisms and a basis for further development of tools for use in maize breeding.
format	article
author	Hanqian Zhang Yuying Qin Kai Huang Fangdong Zhan Ru Li Ru Li Jianjun Chen
author_facet	Hanqian Zhang Yuying Qin Kai Huang Fangdong Zhan Ru Li Ru Li Jianjun Chen
author_sort	Hanqian Zhang
title	Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress
title_short	Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress
title_full	Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress
title_fullStr	Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress
title_full_unstemmed	Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress
title_sort	root metabolite differences in two maize varieties under lead (pb) stress
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8df193f1b15846bd95bc9a819435d4df
work_keys_str_mv	AT hanqianzhang rootmetabolitedifferencesintwomaizevarietiesunderleadpbstress AT yuyingqin rootmetabolitedifferencesintwomaizevarietiesunderleadpbstress AT kaihuang rootmetabolitedifferencesintwomaizevarietiesunderleadpbstress AT fangdongzhan rootmetabolitedifferencesintwomaizevarietiesunderleadpbstress AT ruli rootmetabolitedifferencesintwomaizevarietiesunderleadpbstress AT ruli rootmetabolitedifferencesintwomaizevarietiesunderleadpbstress AT jianjunchen rootmetabolitedifferencesintwomaizevarietiesunderleadpbstress
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Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress

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