Biological autoluminescence for assessing oxidative processes in yeast cell cultures

Abstract Nowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low e...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Petra Vahalová, Kateřina Červinková, Michal Cifra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8df2e8167c544f9c8e72a11138fb4be4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8df2e8167c544f9c8e72a11138fb4be4
record_format	dspace
spelling	oai:doaj.org-article:8df2e8167c544f9c8e72a11138fb4be42021-12-02T15:49:53ZBiological autoluminescence for assessing oxidative processes in yeast cell cultures10.1038/s41598-021-89753-92045-2322https://doaj.org/article/8df2e8167c544f9c8e72a11138fb4be42021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89753-9https://doaj.org/toc/2045-2322Abstract Nowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence—BAL). This phenomenon can be potentially used as a non-invasive and low-operational-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology.Petra VahalováKateřina ČervinkováMichal CifraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Petra Vahalová Kateřina Červinková Michal Cifra Biological autoluminescence for assessing oxidative processes in yeast cell cultures
description	Abstract Nowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence—BAL). This phenomenon can be potentially used as a non-invasive and low-operational-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology.
format	article
author	Petra Vahalová Kateřina Červinková Michal Cifra
author_facet	Petra Vahalová Kateřina Červinková Michal Cifra
author_sort	Petra Vahalová
title	Biological autoluminescence for assessing oxidative processes in yeast cell cultures
title_short	Biological autoluminescence for assessing oxidative processes in yeast cell cultures
title_full	Biological autoluminescence for assessing oxidative processes in yeast cell cultures
title_fullStr	Biological autoluminescence for assessing oxidative processes in yeast cell cultures
title_full_unstemmed	Biological autoluminescence for assessing oxidative processes in yeast cell cultures
title_sort	biological autoluminescence for assessing oxidative processes in yeast cell cultures
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8df2e8167c544f9c8e72a11138fb4be4
work_keys_str_mv	AT petravahalova biologicalautoluminescenceforassessingoxidativeprocessesinyeastcellcultures AT katerinacervinkova biologicalautoluminescenceforassessingoxidativeprocessesinyeastcellcultures AT michalcifra biologicalautoluminescenceforassessingoxidativeprocessesinyeastcellcultures
_version_	1718385704526938112

Biological autoluminescence for assessing oxidative processes in yeast cell cultures

Ejemplares similares