LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was a...

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Autores principales:	Muhamad Salman Fareza, Nur Amalia Choironi, Sri Sutji Susilowati, Melina Puspita Rini, Vyola Festihawa, Irfansyah Septian Nur Fauzi, Esti Dyah Utami, Sarmoko Sarmoko
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	callophylum soulattri, stem bark, lc-ms/ms analysis, mcf-7 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/8df345aa78c6410092e321a51ceedea7
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spelling	oai:doaj.org-article:8df345aa78c6410092e321a51ceedea72021-11-15T06:08:46ZLC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark2338-94272338-948610.22146/ijp.1328https://doaj.org/article/8df345aa78c6410092e321a51ceedea72021-09-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1328https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC50 values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth.Muhamad Salman FarezaNur Amalia ChoironiSri Sutji SusilowatiMelina Puspita RiniVyola FestihawaIrfansyah Septian Nur FauziEsti Dyah UtamiSarmoko SarmokoUniversitas Gadjah Madaarticlecallophylum soulattri, stem bark, lc-ms/ms analysis, mcf-7Pharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 356-364 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	callophylum soulattri, stem bark, lc-ms/ms analysis, mcf-7 Pharmacy and materia medica RS1-441
spellingShingle	callophylum soulattri, stem bark, lc-ms/ms analysis, mcf-7 Pharmacy and materia medica RS1-441 Muhamad Salman Fareza Nur Amalia Choironi Sri Sutji Susilowati Melina Puspita Rini Vyola Festihawa Irfansyah Septian Nur Fauzi Esti Dyah Utami Sarmoko Sarmoko LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark
description	Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC50 values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth.
format	article
author	Muhamad Salman Fareza Nur Amalia Choironi Sri Sutji Susilowati Melina Puspita Rini Vyola Festihawa Irfansyah Septian Nur Fauzi Esti Dyah Utami Sarmoko Sarmoko
author_facet	Muhamad Salman Fareza Nur Amalia Choironi Sri Sutji Susilowati Melina Puspita Rini Vyola Festihawa Irfansyah Septian Nur Fauzi Esti Dyah Utami Sarmoko Sarmoko
author_sort	Muhamad Salman Fareza
title	LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark
title_short	LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark
title_full	LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark
title_fullStr	LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark
title_full_unstemmed	LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark
title_sort	lc-ms/ms and cytotoxic activity analysis of extract and fraction of calophyllum soulattri stembark
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/8df345aa78c6410092e321a51ceedea7
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LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

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